Nikon ECLIPSE TS100 Instructions Manual page 40

Poor image quality (No effect of phase contrast; poor
Causes
(For phase contrast microscopy:)
No annular diaphragm is in optical path.
(For phase contrast microscopy:)
Ph codes of objective and annular
diaphragm do not match.
(For phase contrast microscopy:)
Annular diaphragm image is not
centered on phase ring.
(For phase contrast microscopy:)
Aperture diaphragm is closed.
(For NAMC microscopy:)
No NAMC objective is in optical path.
(For NAMC microscopy:)
NAMC codes of objective and slit
diaphragm do not match.
(For NAMC microscopy:)
Slit diaphragm image is not adjusted to
be contained in G area of modulator.
Condenser position is wrong.
A specimen of culture container bottom
thickness (slide glass thickness) or cover
glass thickness specified on the
objective is not used. Or the specimen is
upside down.
Correction ring of objective is not in
right position (when using objectives
with correction rings).
Lens is dirty. (Condenser, objective, or
eyepiece)
No immersion oil is applied to the tip of
an oil-immersion objective.
The Nikon immersion oil is not used.
The immersion oil contains air bubbles.
Immersion oil adhering to the tip of the
dry-type objective.
(especially, 40x objective.)
6 Troubleshooting
1 Optical
contrast or resolution)
E-39
Corrective Measures
Use an annular diaphragm that matches
the objective. (P.18)
Use an objective and annular diaphragm
having the same Ph code.
Center the annular diaphragm. (P.18)
Fully open the aperture diaphragm.
(P.17)
Place an NAMC objective in optical path.
Use an objective and slit diaphragm
having the same NAMC code.
Adjust the position of the slit diaphragm.
(P.20)
Check for proper attachment to
illumination pillar.
Place a culture container (slide glass)
thickness of around 1.2 mm and cover
glass thickness of 0.17 mm. Place a
specimen of the specified thickness faces
the objective. (P.27)
Adjust the correction ring to match
container thickness. (P.27)
Clean the lens. (P.46)
Apply the Nikon immersion oil. (P.27)
Use the Nikon immersion oil.
Remove the air bubbles. (P.28)
Clean it as appropriate. (P.28)