Nikon ECLIPSE TS100 Instructions Manual page 19

5
Center the annular diaphragm.
(Only when using a centerable phase
slider.)
Center the annular diaphragm so that it aligns with
the phase ring of the objective. Perform this
adjustment with the center of the container (having
an even thickness) in the optical path. Avoid the
periphery of the container.
(1) Place the 10x phase contrast objective (Ph1) in
the optical path.
(2) Slide the phase slider to place the Ph1 annular
diaphragm in the optical path.
(3) Taking care not to alter the position of the
diopter adjustment ring, remove one eyepiece
from the eyepiece tube and insert a centering
telescope in its place.
(4) Holding down the flange of the centering
telescope, turn the eyepiece of the centering
telescope and focus on the objective phase ring.
(5) Insert two hexagonal screwdrivers into the
centering holes on the phase slider and, while
looking through the centering telescope, center
the Ph1 annular diaphragm image so that it
aligns with the phase ring.
(6) If you are using an objective with a Ph code
other than Ph1, place that objective and the
annular diaphragm having the same Ph code as
that objective in the optical path, and center the
annular diaphragm in the same manner
described above.
6
Select the desired phase contrast objective.
Turn the revolving nosepiece and place the phase
contrast objective of the desired magnification in
the optical path. (Turn the revolving nosepiece until
you feel it click in place.)
7
Place the annular diaphragm
corresponding to the objective in the
optical path.
Place the annular diaphragm having the same Ph code
as the objective in the optical path and observe. Since
optical conditions worsen by the difference in the
thickness of the container depending on the location,
the center of the annular diaphragm may become
displaced. If the contrast suddenly worsens, check the
condition of the setup and, if necessary, center the
annular diaphragm again.
8
Adjust the correction ring on the objective.
If the objective has a correction ring, adjust the
ring according to the thickness of the container. For
details, see P.27.
3 Microscopy
1-1 Phase Contrast Microscopy
E-18
Eyepiece
Annular diaphragm
image
Place the annular diaphragm having
the same Ph code as the objective in
the optical path.
Centering
telescope
Flange
Phase ring image