Nikon ECLIPSE TS100 Instructions Manual page 29

Inverted microscope
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Take care not to allow air bubbles
When filling with oil, take care that no air bubbles form inside the oil. The presence of air
bubbles degrades the viewed image. To check for air bubbles, remove one eyepiece, fully open
the aperture diaphragm, and look at the exit pupil of the objective (appears bright round). If it is
difficult to see, remove one of the eyepieces, attach a centering telescope in its place, and
observe while changing the focus by rotating the eyepiece of the centering telescope.
If there are bubbles in the oil, remove them by one of the following methods:
• Turn the revolving nosepiece slightly, moving the oil-immersed objective back and forth once
or twice.
• Add more oil.
• Remove the oil and replace it with new oil.
Handling immersion oil
Use as little oil as possible. If too much oil is applied, the excess will flow onto the stage and
other parts.
Any oil remaining on an oil-immersion type of objective or staining on the tip of a dry type of
objective has a negative effect on viewing. After using oil, wipe all of it away and make sure that
there is no oil on the tips of the other objectives.
To wipe away oil, wipe lightly 3 or 4 times using lens tissue dampened with absolute alcohol
(ethyl alcohol or methyl alcohol). Items may be wiped more cleanly by making sure not to wipe
with the same part of the tissue more than once. A nice finish may be obtained by wiping again
with dry lens tissue.
WARNING
When using absolute alcohol, always follow the instructions provided by the
manufacturer. Keep these flammable liquids away from fire or sparks.
Precautions when using immersion oil
• Make sure to securely tighten the cap after use. After adding oil to the container, securely
tighten the base cap. Since the base cap may become loose by itself after having been used
for a long time, periodically check to make sure it is tight. Oil may leak out if the cap is loose.
• Do not press on the container with excessive force. This may cause the oil suddenly spray
from the container.
• Wipe off any oil that collects on the outside of the container during the course of use.
• Be careful so that oil does not get in your eyes. Although the Nikon immersion oil is not toxic,
apply the first-aid measures listed below if it should come in contact with the skin or enter
the eyes.
♦ Contact with skin:
♦ Enters the eyes:
• Do not place immersion oil in a location exposed to outside light (or UV light).
4 Operation of Each Part
5 Objectives
Completely wash off with soap and water.
Rinse thoroughly with pure water (for at least 15 minutes) and
seek medical attention.
E-28

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