Partec CyFlow Cube 6 Manuals

Partec CyFlow Cube 6 Manuals

Manuals and User Guides for Partec CyFlow Cube 6. We have 2 Partec CyFlow Cube 6 manuals available for free PDF download: Operating Manual, Instrument Operating Manual

Partec CyFlow Cube 6 Operating Manual

Partec CyFlow Cube 6 Operating Manual (40 pages)

Brand: Partec | Category: Measuring Instruments | Size: 2 MB

Table of Contents

Table of Contents
3
Foreword

5
Presentation

6
- Basics
  6
- I N Flow Cytometry
  7
- N Flow Cytometry What Is
  7
  - A Parameter
    7
  - A One-Parameter Histogram
    7
  - A Histogram Channel
    7
  - The Count in a Histogram
    7
  - A Peak
    7
  - Background in a Histogram
    7
  - Example of a Histogram
    8
  - Example of a Dotplot
    8
  - Histogram and Dotplot in Immunology
    9
- Instrument Starting Procedure
  10
  - Sheath Fluid Level Control And/Or Refill
    10
- Main Cyview ™ Login Window
  11
- User Levels
  12
- Cyview™ Controls
  14
  - 1D/2D PLOTS- Display Options
    14
  - Results
    15
  - Regions
    16
  - GENERAL - Properties of the Instrument
    16
  - ACQUISITION - Instrument Control
    17
  - MEASURE - Definition of Measure Modes
    17
  - Measure Modes
    18
  - Analyze All (Default Selection)
    18
  - Volumetric Counting with Volume
    18
  - Volumetric Counting with Electrodes
    19
  - Cells in Region
    19
  - Cyview® Instrument/Measurement Settings
    20
- Prime/Work/Clean
  20
  - Priming / Initialization Process
    20
  - Work Process
    22
  - Shut down Process
    22
- Intermediate Cleaning Process
  23
- Sheath and Waste Bottle
  23
- Measurement Parameters
  24
  - Trigger Properties (P)
    24
  - Threshold Settings (T)
    24
  - Flow Speed (S)
    24
  - Light Source (L)
    24
- 1D/2D Plot Properties
  25
  - Plot Name
    25
  - Log On/Y Log on
    25
  - Erosion Levels
    25
  - X/Y Channel
    25
  - Bitrange
    25
  - CR-Mode
    25
- Region/Roi Properties
  26
  - Create a Polygonal Region
    26
  - Region Properties
    26
  - Region Name
    26
  - Home Plot Name
    26
  - Color RGB
    26
  - Max Count
    26
  - Sorter Region
    26
  - Moving of Regions Within a Histogram
    26
  - Create a Vertical or Horizontal Histogram Splitter
    27
  - Create a Quadrant Assembly
    27
  - Change Layout for Regions
    27
  - Applying Regions to Other Plots  Gating Function
    27
- Compensation
  28
- Typical Sample Analysis
  29
Keyboard/Mouse Combinations

31
Appendix

32
- Biohazards
  32
- Maintenance
  33
  - Service
    33
  - Transport and Storage
    34
  - Disposal
    34
- Laser Safety
  35
- Technical Specifications
  
  36
  - Optical Standard Setup
    36
Notes

39

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Partec CyFlow Cube 6 Instrument Operating Manual

Partec CyFlow Cube 6 Instrument Operating Manual (36 pages)

Brand: Partec | Category: Laboratory Equipment | Size: 8 MB

Table of Contents

Table of Contents
3
Foreword

5
Presentation

6
- Basics
  6
- In Flow Cytometry, What Is
  7
  - A Parameter
    7
  - A One-Parameter Histogram
    7
  - A Histogram Channel
    7
  - The Count in a Histogram
    7
  - A Peak
    7
  - Background in a Histogram
    8
    
    The Lower Level (L-L) or Threshold
    8
  - Example of a Histogram
    9
  - Example of a Dotplot
    9
  - Histogram and Dotplot in Immunology
    10
Getting Started

10
- Instrument Starting Procedure
  11
  - Sheath Fluid Level Control And/Or Refill
    11
    
    Switching on the Cyflow ® Cube
    11
- Main Cyview Login Window
  12
- User Levels
  13
- Cyview™ Main Page
  14
  - Presentation
    14
  - Cyview™ Controls
    15
    
    CHANNELS - Parameter Definition
    15
    
    PLOTS - Display Options
    15
    
    PROCCESS - Instrument Control
    17
Typical Sample Analysis

19
- Starting a Measure
  19
- Measure Modes
  20
- Cleaning Procedures
  21
- Instrument Settings
  22
  - Measurement Parameters
    22
    
    Gains Settings
    22
    
    Threshold Settings (T)
    22
    
    Light Source (L)
    22
    
    Trigger Properties (P)
    22
    
    Flow Speed (S)
    22
    
    Plot Name
    23
  - Plot Properties
    23
    
    Log On/Y Log on
    23
    
    Erosion Levels
    23
    
    X/Y Channel
    23
    
    Mode
    23
    
    Bitrange
    23
    
    CR-Mode
    23
    
    Region Name
    24
    
    Home Plot Name
    24
    
    Colour RGB
    24
    
    Max Count
    24
    
    Sorter Region
    24
    
    Colour Gating on
    24
    
    Moving of Regions Within a Histogram
    24
    
    Create a Polygonal Region
    24
    
    Region Properties
    24
    
    Create a Vertical Histogram Splitter
    25
    
    Create a Vertical Dotplot Splitter
    25
    
    Create a Horizontal Dotplot Splitter
    25
    
    Create a Quadrant Assembly
    25
    
    Change Layout for Regions
    25
    
    Applying Regions to Other Plots Gating Function
    26
  - Keyboard/Mouse Combinations
    27
Appendix

28
- Biohazards
  28
- Maintenance
  29
  - Service
    29
  - Transport and Storage
    30
  - Disposal
    30
- Laser Safety
  31
- Technical Specifications
  32
  - Optical Standard Setup
    32
    
    The Parameters
    32
Notes

35

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