Background In A Histogram; The Lower Level (L-L) Or Threshold - Partec CyFlow Cube Series Instrument Operating Manual

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CyFlow Cube Instrument Operating Manual

... background in a histogram?

Histograms sometimes show undesired signals in the lower channels, frequently called ´noise´ or
´background´. These signals may originate from cell fragments or other particles resulting from sample
preparation. In case of high signal amplification, background can also be caused by particle contaminated
sheath fluid.

The lower level (L-L) or threshold?

The lower level (L-L) threshold is a mean to suppress background signals. Signals below the lower level are
rejected from the signal acquisition. To exclude noise from a histogram already acquired, a region-gate can
be used.
Rev018_2012-01-27
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