Histogram And Dotplot In Immunology; Getting Started - Partec CyFlow Cube Series Instrument Operating Manual

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CyFlow Cube Instrument Operating Manual

Histogram and dotplot in immunology

Lysed blood 2D dotplot
Histogram and dotplot of immunological staining
Slightly pos.
Neg.

Getting Started

G
M
Rev018_2012-01-27
G
M
L
Pos.
L
Lysed blood is represented in a dot plot
presenting the FSC in X axis and SSC in Y axis.
Both axes are in a linear scale.
Three distinct groups are visible; they represent
the granulocytes
lymphocytes (L).
This histogram represents the spectrum of the
cells presented in the previous dot plot stained
with antibodies
PhycoErytrin (PE). The X axis displays the
fluorescence in a 4-dec logarithmic scale and the
Y axis displays the number of events in a linear
scale.
This dot plot presents the cells fluorescence in X
on a logarithmic scale (CD45 FITC) versus SSC
in Y on a linear scale.
This data display allows an easier interpretation of
2 parametric data compared to the histogram.
Concluded from this example, the lymphocytes
are strongly stained with the anti-CD45 antibody,
the monocytes are slightly stained and the
population of granulocytes is negative.
This example is a simplification of an immune-
staining analysis by flow cytometry. It is
directed to new users, and aims to link the
histogram to the dotplot representation. To
complete the analysis, gating and statistics
are required.
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(G), monocytes (M)
anti-CD16 conjugated
and
to