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Summary of Contents for Partec CyFlow Cube Series
- Page 1 ® CyFlow Cube Instrument Operating Manual ® ® ® Cube 6 │ CyFlow Cube 8 │ CyFlow CyFlow Cube 8 Sorter...
- Page 2 Cube Flow Cytometer complies with the European IVD Directive 98/79/EC and is therefore CE marked. For in vitro diagnostic use with Partec recommended IVD reagents. Contact Information: Partec GmbH • Am Flugplatz 13 • D-02828 Görlitz • Germany Tel +49 3581 8746 0 • Fax +49 3581 8746 70 E-mail: service@partec.com ©...
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Page 3: Table Of Contents
Cube Instrument Operating Manual Table of Content FOREWORD ........................5 PRESENTATION ......................6 ......................................6 ASICS What is the Partec CyFlow ® Cube? ............................6 What are the applications for which the CyFlow ® Cube can be used? ..............6 What are topics covered by this manual? ......................... - Page 4 ® CyFlow Cube Instrument Operating Manual ................................22 NSTRUMENT SETTINGS Measurement parameters ..............................22 Gains settings ............................................ 22 Threshold settings (T) ........................................22 Light source (L) ..........................................22 Trigger properties (P) ........................................22 Flow speed (S) ..........................................22 Plot name ............................................23 Plot properties....................................
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Page 5: Foreword
CyView or you have a good suggestion to be included in a new version, please let us know by sending an email or a note to Partec GmbH. ® For more details about the reagent kits, suitable for use with the CyFlow Cube, please refer to the respective product data sheets. -
Page 6: Presentation
® CyFlow Cube Instrument Operating Manual Presentation Basics ® What is the Partec CyFlow Cube? ® ® The Partec CyFlow Cube is a fully equipped desktop Flow Cytometer (FCM). CyFlow Cube features a modular optical concept. This allows using different lasers as light sources and the detection of up to 8 ®... -
Page 7: In Flow Cytometry, What Is
® CyFlow Cube Instrument Operating Manual In flow cytometry, what is ... … a parameter? In flow cytometry, parameter denotes a measured property of the particles. Frequently, a parameter is synonymous to an optical channel. E.g. an instrument with 6 parameters is equipped with 6 optical detectors. -
Page 8: Background In A Histogram
® CyFlow Cube Instrument Operating Manual … background in a histogram? Histograms sometimes show undesired signals in the lower channels, frequently called ´noise´ or ´background´. These signals may originate from cell fragments or other particles resulting from sample preparation. In case of high signal amplification, background can also be caused by particle contaminated sheath fluid. -
Page 9: Example Of A Histogram
® CyFlow Cube Instrument Operating Manual Example of a histogram A histogram represents a distribution of measured signals (events) over 1 dimension. Data can be presented on the dimensions of relative particle size or optical particle structure (Forward Scatter Number of events (FCS) or Side Scatter (SSC)), resp. -
Page 10: Histogram And Dotplot In Immunology
® CyFlow Cube Instrument Operating Manual Histogram and dotplot in immunology Lysed blood 2D dotplot Lysed blood is represented in a dot plot presenting the FSC in X axis and SSC in Y axis. Both axes are in a linear scale. Three distinct groups are visible;... -
Page 11: Instrument Starting Procedure
In order to guarantee highest quality of the measurements we highly recommend use Partec Sheath Fluid (order no. 04-4007). It is recommended to replace the sheath fluid at... -
Page 12: Main Cyview Login Window
® CyFlow Cube Instrument Operating Manual ™ Main CyView login window User name Password The log in window allows to start the software at the USER, MAIN USER or SERVICE level. During start of the instrument the automated self- testing procedures are processed: XML-configuration displays the correct loading of the setting files... -
Page 13: User Levels
User levels Three different user levels exist: SERVICE: Restricted to authorized Partec trained persons and for service purposes only MAIN USER: Complete functionality of the instrument, method development Main users can create new accounts of the Main User and User level... -
Page 14: Cyview™ Main Page
® CyFlow Cube Instrument Operating Manual CyView™ Main Page Presentation File operations Instrument settings Region statistics Results table Console Real time PC/control instrument board link work load Measuremen t interface The main window will be your interface to acquire, save, re-load and analyse your data. Instrument real time display of workload (L), on-board memory status (M), analysis volume (V) and analysis duration (D). -
Page 15: Cyview™ Controls
® CyFlow Cube Instrument Operating Manual CyView™ Controls CHANNELS – parameter definition The CHANNELS register defines properties of PMT channels (the parameters) present in the systems (PMT0 – PMT7). It names the parameters (FSC, SSC, …FLx) defines Color Crosstalk Compensation. Confirm all modifications by pressing Accept PLOTS –... - Page 16 ® CyFlow Cube Instrument Operating Manual RESULTS The RESULTS register defines properties of calculated results displayed in the RESULTS table. Properties of result calculations It is possible to set up calculations with the COUNT of individual regions according to the specified formula: NumReg1 (+ - x / ) NumReg2 --------------------------------------------------x Scale DenomReg1 (+ - x / ) DenomReg2...
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Page 17: Proccess - Instrument Control
® CyFlow Cube Instrument Operating Manual SYSTEM - properties of the instrument The active Configuration Script The instruments Serial Number Total Operating Time of the instrument Version of the instrument Modification of the instrument Activate Autostart function Activates Sorter function Defines a factor for sample Dilution Clinic/Customer defines specific User information... - Page 18 ® CyFlow Cube Instrument Operating Manual SCRIPT – definition of measure modes Measmode allows selection of: - Continuous analysis - Volumetric Counting with Electrodes - Volumetric Counting with Volume - Events in Region Speed values and Volume values can be edited and stored in FCS file and Config script Confirm all modifications by pressing Accept Schematic overwiew of the measure modes with.
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Page 19: Typical Sample Analysis
® CyFlow Cube Instrument Operating Manual Typical Sample Analysis A step by step procedure and what are the important steps in the data acquisition. Starting a measure During the data acquisition the total particle count and the analysis rate is indicated as a sliding bar. During the acquisition phase it is possible to Load a sample demo file (data files can be manipulate the instruments set-up e.g. -
Page 20: Measure Modes
® CyFlow Cube Instrument Operating Manual Measure modes The following measure modes can be selected prior to start of an analysis: Continuous Volumetric counting with electrodes Volumetric counting with volume Events in region Continous (default selection) This default mode will allow you to run your sample until its levels reached the stop electrode. Volumetric Counting with Electrodes This measuring mode uses the START and STOP electrodes of the sample port to define a fixed sample volume. -
Page 21: Cleaning Procedures
® CyFlow Cube Instrument Operating Manual Cleaning Procedures Cleaning procedure during Cube running The Cube can be cleaned between sets of samples using the cleaning and rinsing solution (green solution, Order No 04-4009). This procedures will allow you to reduce significantly the cross contamination and reduce the background. -
Page 22: Instrument Settings
® CyFlow Cube Instrument Operating Manual Instrument settings Measurement parameters The PROCESS register can be opened by clicking on the button Meas ( Gains settings The GAIN (G) and THRESHOLD (T) sliders must be adjusted to obtain the optimal gain (maximum signal and minimum background). -
Page 23: Plot Name
® CyFlow Cube Instrument Operating Manual Plot properties Plot name A default plot name (Hx, Px) is defined in the script. Default names of the Parameters will be displayed. The user can modify the parameter name (line comment) enter a parameter name better matching the experiment. -
Page 24: Region Name
® CyFlow Cube Instrument Operating Manual Region/ROI properties Create a polygonal region Region name Double left click on the plot where the gate/region As default, the gate will be named: region nx, is required, a first point will be set; a second click successive region: region nx+1 will set a further point, and so on. -
Page 25: Create A Vertical Histogram Splitter
® CyFlow Cube Instrument Operating Manual Create a vertical histogram splitter To create a vertical histogram splitters please move the cursor into the histogram and press the right mouse button. Select “Built vertical splitter region” to divide the histogram into two sections (VS1-1 and VS1-2). -
Page 26: Applying Regions To Other Plots Gating Function
® CyFlow Cube Instrument Operating Manual Applying regions to other plots Gating Function To select an existing region for gating select the respective region with the right mouse button and select “Color gating on”. Clicking into any histogram or plot with the right mouse button (outside a region area) allows to select any existing region which is labeled with “Color gating on”. -
Page 27: Keyboard/Mouse Combinations
® CyFlow Cube Instrument Operating Manual Keyboard/Mouse combinations Context Action Effect Graphic Plot Mouse double-click Opens edit function for regions Right Mouse-click Ends creation of a region Create Region Left Mouse-click Sets dot of a region click and hold left mouse Moves a region in standard steps click and hold left mouse Moves a region in small steps... -
Page 28: Appendix
® CyFlow Cube Instrument Operating Manual Appendix Biohazards Warning: The Waste may contain biohazardous and carcinogenic material from the samples (infectious material, dyes). Please note: Strict guidelines, international, as well as national regulatory standards such as GLP (good laboratory practice) must be met for all users. Therefore, the system is marked with the following biohazard label: Warning: biohazards Rev018_2012-01-27... -
Page 29: Maintenance
Clean waste and sheath reservoir, wipe top dry. Service All service is to be made from an authorized service engineer. Please contact your local supplier or Partec (service@partec.com). For further information, please consult our web site:... -
Page 30: Transport And Storage
Warning: The Waste may contain biohazardous and carcinogenic material from the samples (infectious material, dyes). In case of product disposal, please proceed according to the Partec standard operating procedure (SOP) for decontamination. After decontamination, the system has to be disposed according to the local regulations and laws. -
Page 31: Laser Safety
® CyFlow Cube Instrument Operating Manual Laser Safety Warning: It is prohibited to open the instrument as it is equipped with a class 3b laser unit. ® The CyFlow Cube is a class I laser product according to the EN 60825-1:2007. Please note: Laser light can be emitted if the housing of the device is damaged and the protection cover for the laser beam is removed. -
Page 32: Technical Specifications
® CyFlow Cube Instrument Operating Manual Technical Specifications Note: Due to fast technological improvements, specifications herein are subject to change. For details, please inquire information from your local supplier. Optical Standard Setup The Parameters If required by a specific application, the optical standard setup can be optimized by exchanging FSC: forward scatter... - Page 33 Based on precise counting and mechanical fluid volume measurement Absolute Counting No need for reference sample or beads Instrument Check Partec Count Check Beads Partec Calibration Beads 1 µm and 3 µm Partec DNA Control PI Set-up Time Max. 5 minutes Parameters...
- Page 34 ® CyFlow Cube Instrument Operating Manual ® 2. CyFlow Cube Optics Laser / Output Red Diode Laser: 25 mW at 635 nm / 40 mW at 640 nm Green NdYAG: 30 mW to 100 mW at 532 nm Blue solid-state Laser: 20 mW at 488 nm Blue laser diode: 50 mW at 488 nm...
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Page 35: Notes
® CyFlow Cube Instrument Operating Manual Notes Rev018_2012-01-27 35/36... - Page 36 ® CyFlow Cube Instrument Operating Manual Rev018_2012-01-27 Excellence in Flow Cytometry and Cell Analysis 36/36...
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