Typical Sample Analysis; Starting A Measure - Partec CyFlow Cube Series Instrument Operating Manual

Typical Sample Analysis

A step by step procedure and what are the important steps in the data acquisition.

Starting a measure

Load a sample demo file (data files can be
selected from the folder
(C:/CyView_85\data\xxxxx.fcs). The respective
instrument settings are automatically loaded.
Fill a sample tube with 1.2 ml of calibration beads.
Check the tube for eventual imperfection or
contaminants (cracks, aggregates, hair) and
replace or remove, resp. if necessary.
Insert the tube into the sample port, push it up
until a distinct click is heard
Press the start button. In the console window,
information on the status of the measurement is
displayed. The script work is proceeding step by
step.
CyView™ StartPrerun
Prerun and stabilization of sample flow
Start Measure
Data acquisition
Console displaying control board status
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CyFlow Cube Instrument Operating Manual
During the data acquisition the total particle count
and the analysis rate is indicated as a sliding bar.
During the acquisition phase it is possible to
manipulate the instruments set-up e.g. by
changing samples speed, PMT voltage and
threshold levels. Use CLEAR button
to erase data after manipulations of the
instruments set-up.
By default, the continuous measure mode is pre-
selected. In this case the measurement will only
stop automatically when the sample is consumed.
An earlier stop can be realized by pressing the
STOP button . Data can be saved with
The configuration file can be saved with
Besides the Continous measure mode other
measure modes can be selected:
Volumetric counting with volume
A volume can be pre-selected
Volumetric counting with electrodes
The volumetric counting is based on the
electrode status
Events in region
A particle number can be pre-selected
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Rev018_2012-01-27