Table Of Contents - Partec CyFlow Cube Series Instrument Operating Manual

Table of Content
FOREWORD ................................................................................................................... 5
PRESENTATION ............................................................................................................. 6
B ........................................................................................................................................................................................... 6
ASICS
What is the Partec CyFlow
What are the applications for which the CyFlow
What are topics covered by this manual? ......................................................................................................................... 6
What other manuals are available? ..................................................................................................................................... 6
What should I know before operating the CyFlow
I ,
N FLOW CYTOMETRY
... a parameter? .............................................................................................................................................................................. 7
... a one-parameter histogram? ............................................................................................................................................. 7
... a histogram channel? ............................................................................................................................................................. 7
... the count in a histogram? .................................................................................................................................................... 7
... a peak? .......................................................................................................................................................................................... 7
... background in a histogram? ............................................................................................................................................... 8
The lower level (L-L) or threshold? ..........................................................................................................................................................................8
Example of a histogram ............................................................................................................................................................. 9
Example of a dotplot ................................................................................................................................................................... 9
Histogram and dotplot in immunology ............................................................................................................................ 10
GETTING STARTED ..................................................................................................... 10
I
NSTRUMENT STARTING PROCEDURE
Sheath fluid level control and/or refill ............................................................................................................................. 11
Switching on the CyFlow
M C V ™
AIN Y IEW LOGIN WINDOW
U ............................................................................................................................................................................... 12
SER LEVELS
C V ™ M P
Y IEW AIN AGE
Presentation .................................................................................................................................................................................. 14
CyView™ Controls ....................................................................................................................................................................... 15
CHANNELS - parameter definition ....................................................................................................................................................................... 15
PLOTS - display options ............................................................................................................................................................................................. 15
PROCCESS - instrument control .............................................................................................................................................................................. 17
TYPICAL SAMPLE ANALYSIS .................................................................................... 19
S ............................................................................................................................................................... 19
TARTING A MEASURE
M ....................................................................................................................................................................... 20
EASURE MODES
Continous (default selection) ................................................................................................................................................ 20
Volumetric Counting with Electrodes ............................................................................................................................... 20
Volumetric Counting with Volume ..................................................................................................................................... 20
Events in Region .......................................................................................................................................................................... 20
C P
LEANING ROCEDURES
Cleaning procedure during Cube running ....................................................................................................................... 21
Cleaning procedure for switching off ................................................................................................................................ 21
No cross contamination between samples ...................................................................................................................... 21
® Cube? ....................................................................................................................................... 6
... ............................................................................................................................................. 7
WHAT IS
..................................................................................................................................... 11
® Cube .............................................................................................................................................................................. 11
............................................................................................................................................. 12
.............................................................................................................................................................. 14
........................................................................................................................................................... 21
®
CyFlow Cube Instrument Operating Manual
® Cube can be used? ................................................................. 6
® Cube? ....................................................................................... 6
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Rev018_2012-01-27