Region Name; Home Plot Name; Colour Rgb; Max Count - Partec CyFlow Cube Series Instrument Operating Manual

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CyFlow Cube Instrument Operating Manual
Region/ROI properties

Create a polygonal region

Double left click on the plot where the gate/region
is required, a first point will be set; a second click
will set a further point, and so on. Label as many
points as required with the left mouse button. To
close the gate surface, click mouse right.

Region properties

To access the region property, Ctrl+right-click on
the gate to edited. On the PLOTS register on the
right of the screen a selection of region options
are displayed.
Rev018_2012-01-27

Region name

As default, the gate will be named: region nx,
successive region: region nx+1
The user can also define a name. Remember to
validate by Accept before to move on another
region.

Home Plot name

This defines the physical plot where the region is
located.

Colour RGB

Allows to choose the colour of the region. This
colour will be reported to each plot selected as
colour gating only, illustrated in the graph on the
left hand side with an overlay of the staining of
lymphocytes in red overlaying the blue histogram
presenting all events.

Max count

This option allows to fix the maximum number of
events from a particular ROI (Region Of Interest)
to be analysed (see CyView™ 8 SCRIPT
register).

Sorter region

To set the ROI/region as a sorting gate, just by
selecting the box.
(This option is only available on the Cube
equipped with Sorter flow cuvette).

Colour Gating On

Selecting this option willallow the user to apply
this particular gate to another plot.

Moving of regions within a histogram

Move the cursor into the region and keep the left
mouse button pressed while moving the position.
Individual points of a region can be changed by
approaching with the cursor to the point and keep
the left mouse button pressed during movement.
Regions within a dotplot can be changed in size
by selecting the region with the cursor and
pressing the "Shift"
movement
Each region can be selected with the right mouse
button for deletion.
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button during cursor