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CyFlow
Cube Instrument Operating Manual
Instrument settings
Measurement parameters
The PROCESS register can be opened by clicking on the button Meas (
Flow speed (S)
This slider allows you to change the sample
.
injection speed into the flow cuvette. Low speed
values results in a better precision and accuracy.
A higher speed can be used when the particle
concentration is measured and accuracy is of
lower relevance.
Example of single (1) and multiple trigger (2)
in FSC, and FSC+SSC.
Rev018_2012-01-27
Gains settings
P
The GAIN (G) and THRESHOLD (T) sliders must
be adjusted to obtain the optimal gain (maximum
G
signal and minimum background). Typically, the
Forward Scatter (FSC) will be the first gain tuned
to adjust the size of the studied particles.
To move a slider, click mouse left on the slider,
keep mouse button down and move mouse left –
right or use the scroll wheel to adjust the value.
Threshold settings (T)
The threshold allows cutting off background by
T
setting a lower limit of the acquired data in the
trigger parameter. This tool allows to increase the
accuracy and precision of the acquired data.
Light source (L)
This option allows to switch on/off the light
sources.
S
Trigger properties (P)
L
Single trigger:
The trigger is the parameter defining if a
signal gets recorded. Only if the trigger
parameter detects a signal, other parameters
of the system will record signals. In other
words, if a trigger is set in FSC to record only
bigger particles (e.g. threshold set at 0.3871V
with a gain value of 170V), e.g. only intact
mammalian cells will be acquired. Cell debris
and smaller particles will be excluded as long
as their FSC signal remains below the trigger
threshold.
1
Multiple trigger:
The option allows to use multiple trigger
parameters
2
22/36
)
for
signal
acquisition.
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