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Table of Contents
Related Manuals for Partec CyFlow Cube 6
Summary of Contents for Partec CyFlow Cube 6
- Page 1 ® CyFlow Cube 6 Instrument Operating Manual ® CyFlow Cube 6...
- Page 2 ® CyFlow Cube 8 - Instrument Operating Manual For in vitro diagnostic use with Partec recommended IVD reagents. ® The PartecCyFlow Cube Flow Cytometer complies with the European IVD Directive 98/79/EC and is therefore CE marked. Contact Information: Partec GmbH • Otto-Hahn-Straß3 32 • D-48161 Münster • Germany Tel +49 2534 8008 0 •...
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Page 3: Table Of Contents
Cube Instrument Operating Manual Table of content FOREWORD ........................5 PRESENTATION ......................6 ......................................6 ASICS What is the Partec CyFlow Cube? ............................6 ® What are the applications for which the CyFlow Cube can be used? ..............6 ®... - Page 4 ® CyFlow Cube 8 - Instrument Operating Manual Trigger properties (P) ................................24 Threshold settings (T) ................................24 Flow speed (S) ....................................24 Light source (L) ................................... 24 1D/2D P ............................... 25 LOT PROPERTIES Plot name ......................................25 X Log On/Y Log On ..................................25 Erosion levels ....................................
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Page 5: Foreword
CyView or you have a good suggestion to be included in a new version, please let us know by sending an email or a note to Partec GmbH. ™ The present manual is valid as of CyView software version 1.5. -
Page 6: Presentation
® CyFlow Cube 8 - Instrument Operating Manual Presentation Basics ® What is the Partec CyFlow Cube? ® ® The PartecCyFlow Cube is a fully equipped desktop Flow Cytometer (FCM). CyFlow Cube features a modular optical concept. This allows using different lasers as light sources and the detection of up to 8 ®... -
Page 7: I N Flow Cytometry
® CyFlow Cube Instrument Operating Manual In flow cytometry, what is... … a parameter? In flow cytometry, parameter denotes a measured property of the particles. Frequently, a parameter is synonymous to an optical channel. E.g. an instrument with 6 parameters is equipped with 6 optical detectors. -
Page 8: Example Of A Histogram
® CyFlow Cube 8 - Instrument Operating Manual Example of a histogram A histogram represents a distribution of measured signals (events) over 1 dimension. Data can be presented on the dimensions of relative particle size or optical particle structure (Forward Scatter (FCS) or Side Scatter (SSC)), resp. Number of events or on their relative fluorescence intensities in different light colors (fluorescence parameters FL1 to FL6). -
Page 9: Histogram And Dotplot In Immunology
® CyFlow Cube Instrument Operating Manual Histogram and dotplot in immunology Lysed blood 2D dotplot Lysed blood is represented in a dot plot presenting the FSC in X axis and SSC in Y axis. Both axes are in a linear scale. Three distinct groups are visible;... -
Page 10: Instrument Starting Procedure
Please notice: Higher level of sheath fluid could lead to unstable sample flow. In order to guarantee highest quality of the measurements we highly recommend use Partec Sheath Fluid (order no. 04-4007). It is recommended to replace the sheath fluid at least once a week or before any daily use. -
Page 11: Main Cyview ™ Login Window
® CyFlow Cube Instrument Operating Manual ™ Main CyView login window The log in window allows to start the software at the USER, MAIN USER or SERVICE level. During start of the instrument the automated self-testing procedures are processed: Cleaning up XML-configuration displays the correct loading of the setting files... -
Page 12: User Levels
CyFlow Cube 8 - Instrument Operating Manual User levels Three different user levels exist: SERVICE/ADMINSTRATOR: Restricted to authorized Partec trained persons and for service purposes only MAIN USER: Complete functionality of the instrument, method development Main users can create new accounts of the Main User and User level... - Page 13 ® CyFlow Cube Instrument Operating Manual CyView™ Main Page – after successful log-in File operations Instrument settings Results table Region statistics Real time instrument Console work load PC/control board link Measurement interface and indicators The main window will be your interface to acquire, save, re-load and analyse your data Instrument real time display of workload (L), on-board memory status (M), analysis volume (V) and analysis duration (D).
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Page 14: Cyview™ Controls
® CyFlow Cube 8 - Instrument Operating Manual CyView™ Controls Overview of instrument settings 1D/2D PLOTS– display options The 1D/2D Plots register defines properties of the graphical plots. The basic layout in terms of number of plots, type of plots (histograms, dot plots) and position of the plots is defined in the used Configuration File. -
Page 15: Results
® CyFlow Cube Instrument Operating Manual RESULTS The RESULTS register defines properties of calculated results displayed in the RESULTS table. Properties of result calculations It is possible to set up calculations with the COUNT of individual regions according to the specified formula: NumReg1 (+ - x / ) NumReg2 --------------------------------------------------x Scale DenomReg1 (+ - x / ) DenomReg2... -
Page 16: Regions
® CyFlow Cube 8 - Instrument Operating Manual REGIONS Home Plot Name defines the plot the region refers to Color RGB defines the region´s color and its color in color gating Max Count defines a maximum count for an “Cells in Region”... -
Page 17: Acquisition - Instrument Control
® CyFlow Cube Instrument Operating Manual ACQUISITION – instrument control Select the trigger parameter multiple trigger parameters are logical “or” connections Gain values to change the voltage of the individual optical parameters (0 volt - 999 volts) defines PMT signal amplification Threshold defines the trigger signals cut-off level (on 4 dec log scale) Flow defines the speed of sample injection in µl/s... -
Page 18: Measure Modes
® CyFlow Cube 8 - Instrument Operating Manual Schematic overview of the acquisition process for all measure modes Green and red colored bars refer to particle count indicator of CyView. Please note: In the measure mode “VC of Volume” or “Cells in Regions” there is no cleaning cycle. Subsequently the data can be stored. -
Page 19: Volumetric Counting With Electrodes
® CyFlow Cube Instrument Operating Manual Volumetric Counting with Electrodes This measuring mode uses the START and STOP electrodes of the sample port to define a fixed sample volume. In a standard sample port this “counting volume” is 200 µl. In a pre-counting phase the sample is acquired normally as in continuous acquisition mode. -
Page 20: Cyview® Instrument/Measurement Settings
To start the priming/initialization process, please follow the instructions below: Open a configuration file and select the initialization modus by clicking on the Initialization batch symbol (Partec Master Cfg-file: Prime mode is preset). If you will perform the daily check-up please select the “Calibration Beads” configuration-file. Only in this configuration file you will find the correct settings for the calibration beads. - Page 21 ® CyFlow Cube Instrument Operating Manual Remove the Sample tube, press “Continue” and wait until the system has finished. Connect the Sample tube again, press “Continue” and wait until the system has finished At the end press “Continue”. The instrument will be cleaned and the Priming process ends. Switch to “WORK”...
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Page 22: Work Process
® CyFlow Cube 8 - Instrument Operating Manual Work process Choose the work batch for measure and acquire of data. Shut down process For the Cleaning of the Sample port a separate cleaning mode is available. The detail procedure would be the following. -
Page 23: Intermediate Cleaning Process
® CyFlow Cube Instrument Operating Manual After finishing the cleaning process CyView will be shut down automatically. Laser sources will also be switched off. Switch off the PC Close Windows (Start – Exit- Switch Computer Off) To power off completely, use the main power switch at the back panel Intermediate cleaning process The Cube can be cleaned between sets of samples using cleaning solution (green solution, Order No 04- 4009) followed by a sample tube with sheath fluid. -
Page 24: Measurement Parameters
® CyFlow Cube 8 - Instrument Operating Manual Measurement parameters The Acquisition register can be opened by clicking on the button Meas ( ) Gains settings Trigger properties (P) Single trigger: The trigger is the parameter defining if a signal gets recorded. Only if the trigger parameter detects a signal, other parameters of the system will record signals. -
Page 25: 1D/2D Plot Properties
® CyFlow Cube Instrument Operating Manual 1D/2D Plot properties Plot name A default plot name (Hx, Px) is defined in the file. Default names of the Parameters will be displayed. The user can modify for each plot the parameter name (line comment) enter a name better matching the experiment. -
Page 26: Region/Roi Properties
® CyFlow Cube 8 - Instrument Operating Manual Region/ROI properties Create a polygonal region Double left click on the plot where the gate/region is required, a first point will be set; a second click will set a further point, and so on. Label as many points as required with the left mouse button. -
Page 27: Create A Vertical Or Horizontal Histogram Splitter
® CyFlow Cube Instrument Operating Manual Create a vertical or horizontal histogram splitter To create a vertical or horizontal histogram splitter please move the cursor into the histogram and press the right mouse button. Select “Built vertical splitter region group” / “Built horizontal splitter region group”... -
Page 28: Compensation
® CyFlow Cube 8 - Instrument Operating Manual Show colors regions only Show colors only All + Region Colors Compensation Press Compensation button to open the compensation Compensation: Select a parameter combination to compensate. Use the slide bar to set the compensation. Clear/Save and Load Compensation Confirm by clicking Accept Example of a color-crosstalk compensation of FL1 into FL2. -
Page 29: Typical Sample Analysis
® CyFlow Cube Instrument Operating Manual Typical Sample Analysis A step by step procedure and what are the important steps in the data acquisition. Starting a measurement Load a sample demo file (data files can be selected from the folder: The respective instrument settings are automatically loaded. - Page 30 ® CyFlow Cube 8 - Instrument Operating Manual During the data acquisition the total particle count and the analysis rate is indicated on the real-time count meters During the acquisition (Pre-Measure) phase it is possible to manipulate the instruments set-up e.g. by changing samples speed, PMT voltage and threshold levels.
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Page 31: Keyboard/Mouse Combinations
® CyFlow Cube Instrument Operating Manual Keyboard/Mouse combinations Context Action Effect Graphic Alt + Mouse left-click Opens Plot/Histogram (big) or close (small) Plot/Histogram Create Region Right Mouse-click Ends creation of a region Left Mouse-click Sets dot of a region click and hold left mouse Moves a region in standard steps Ctrl+ Moves a region in small steps... -
Page 32: Appendix
® CyFlow Cube 8 - Instrument Operating Manual Appendix Biohazards Warning: The Waste may contain biohazardous and carcinogenic material from the samples (infectious material, dyes). Please note: Strict guidelines, international, as well as national regulatory standards such as PPE (personal protection equipment) must be met for all users. Therefore, the system is marked with the following biohazardlabel: Warning: biohazards Rev-05_2014-01-31... -
Page 33: Maintenance
Clean waste and sheath reservoir, wipe top dry. Service All service is to be made from an authorized service engineer. Please contact your local supplier or Partec (service@partec.com). For further information, please consult our web site:... -
Page 34: Transport And Storage
Warning: The Waste may contain biohazardous and carcinogenic material from the samples (infectious material, dyes). In case of product disposal, please proceed according to the Partec standard operating procedure (SOP) for decontamination. After decontamination, the system has to be disposed according to the local regulations and laws. -
Page 35: Laser Safety
® CyFlow Cube Instrument Operating Manual Laser Safety Warning: It is prohibited to open the instrument as it is equipped with a class 3b laser unit. ® The CyFlow Cube is a class I laser product according to the EN 60825-1:2007. Please note: Laser light can be emitted if the housing of the device is damaged and the protection cover for the laser beam is removed. -
Page 36: Technical Specifications
® CyFlow Cube 8 - Instrument Operating Manual Technical Specifications Note: Due to fast technological improvements, specifications herein are subject to change. For details, please inquire information from your local supplier. Optical Standard Setup The Parameters If required by a specific application, the optical standard setup can be optimized by exchanging FSC: forward scatter... - Page 37 No need for reference sample or beads Instrument Check Partec Count Check Beads (Order No. 05-4010) Partec Calibration Beads 1 µm (Order No. 05-4007) Partec Calibration Beads 3 µm (Order No. 05-40018) Partec DNA Control PI (Order No. 05-7303) Partec DNA Control UV (Order No. 05-7302) Set-up Time Max.
- Page 38 ® CyFlow Cube 8 - Instrument Operating Manual ® The CyFlow Cube System is a completely closed system not producing any aerosols during cellular analysis. ® 2. CyFlow Cube Optics Laser / Output Red Diode Laser: 25 mW at 635 nm / 40 mW at 640 nm Blue solid-state Laser: 20 mW at 488 nm Detectors...
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Page 39: Notes
® CyFlow Cube Instrument Operating Manual Notes Rev-05_2014-01-31 39/40... - Page 40 Excellence in Flow Cytometry and Cell Analysis...
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