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In flow cytometry, what is ...
... a parameter?
In flow cytometry, parameter denotes a measured
property of the particles. Frequently, a parameter
is synonymous to an optical channel. E.g. an
instrument with 6 parameters is equipped with 6
optical detectors.
... a one-parameter histogram?
A
one- parameter
distribution of cells among a specific property, e.g.
how many cells contain a given quantity of DNA
or bind a given number of antibody molecules.
... a histogram channel?
The measured signal intensity is assigned to one of 65536 quantity classes or channels. In a one-
parameter histogram the channels are represented on the x-axis.
... the count in a histogram?
The number of cells being assigned to a given channel is referred to as channel content or simply count. In
a one-parameter histogram, the count is shown on the y-axis.
... a peak?
All cells having about equal characteristics among the analysed cell property (e.g. content of a specific
constituent like DNA), form a peak. In the case a of typical DNA histogram one peak represents the G1 and
another peak (with twice the channel value) represents the G2/M phase of the cell cycle.
In case of immunolabelled cells often one peak for unlabelled (negative) and one peak for labelled
(positive) cells can be detected. Peaks can be analysed by identifying them with region markers.
histogram
displays
®
CyFlow
the
7/36
Cube Instrument Operating Manual
Rev018_2012-01-27
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