Partec CyFlow Cube 8 Manuals

Manuals and User Guides for Partec CyFlow Cube 8. We have 1 Partec CyFlow Cube 8 manual available for free PDF download: Instrument Operating Manual

Partec CyFlow Cube 8 Instrument Operating Manual

Partec CyFlow Cube 8 Instrument Operating Manual (36 pages)

Brand: Partec | Category: Laboratory Equipment | Size: 8.79 MB

Table of Contents

Table of Contents
3
Foreword

5
Presentation

6
- Basics
  6
- In Flow Cytometry, What Is
  7
  - A Parameter
    7
  - A One-Parameter Histogram
    7
  - A Histogram Channel
    7
  - The Count in a Histogram
    7
  - A Peak
    7
  - Background in a Histogram
    8
    
    The Lower Level (L-L) or Threshold
    8
  - Example of a Histogram
    9
  - Example of a Dotplot
    9
  - Histogram and Dotplot in Immunology
    10
Getting Started

10
- Instrument Starting Procedure
  11
  - Sheath Fluid Level Control And/Or Refill
    11
    
    Switching on the Cyflow ® Cube
    11
- Main Cyview Login Window
  12
- User Levels
  13
- Cyview™ Main Page
  14
  - Presentation
    14
  - Cyview™ Controls
    15
    
    CHANNELS - Parameter Definition
    15
    
    PLOTS - Display Options
    15
    
    PROCCESS - Instrument Control
    17
Typical Sample Analysis

19
- Starting a Measure
  19
- Measure Modes
  20
- Cleaning Procedures
  21
- Instrument Settings
  22
  - Measurement Parameters
    22
    
    Gains Settings
    22
    
    Threshold Settings (T)
    22
    
    Light Source (L)
    22
    
    Trigger Properties (P)
    22
    
    Flow Speed (S)
    22
    
    Plot Name
    23
  - Plot Properties
    23
    
    Log On/Y Log on
    23
    
    Erosion Levels
    23
    
    X/Y Channel
    23
    
    Mode
    23
    
    Bitrange
    23
    
    CR-Mode
    23
    
    Region Name
    24
    
    Home Plot Name
    24
    
    Colour RGB
    24
    
    Max Count
    24
    
    Sorter Region
    24
    
    Colour Gating on
    24
    
    Moving of Regions Within a Histogram
    24
    
    Create a Polygonal Region
    24
    
    Region Properties
    24
    
    Create a Vertical Histogram Splitter
    25
    
    Create a Vertical Dotplot Splitter
    25
    
    Create a Horizontal Dotplot Splitter
    25
    
    Create a Quadrant Assembly
    25
    
    Change Layout for Regions
    25
    
    Applying Regions to Other Plots Gating Function
    26
  - Keyboard/Mouse Combinations
    27
Appendix

28
- Biohazards
  28
- Maintenance
  29
  - Service
    29
  - Transport and Storage
    30
  - Disposal
    30
- Laser Safety
  31
- Technical Specifications
  32
  - Optical Standard Setup
    32
    
    The Parameters
    32
Notes

35

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