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Specifying Wash Cycle Operations
The final step in creating a wash protocol is to specify individual operations
within the wash protocol.
An example of a wash protocol is shown in 45. In this example, the dispense
wash pin s are purged, and then a Dispense and Aspirate are carried out
twice on each of the 12 strips (columns) of a 96-well Microtiter
Finally, after all 12 strips have been washed twice, the contents of the
Microtiter plate are allowed to soak (equilibrate) for another 600 seconds.
PURGE
Purge the dispense pins.
↓
MOVE
Specify the starting strip and ending strip for each cycle
(2 Cycles)
and the number of cycles to be performed.
↓
DISPENSE
(3 Times)
↓
ASPIRATE
↓
END LOOP
↓
After the two Move cycles have been completed, allow
SOAK
the contents to equilibrate for 600 seconds.
↓
END
End the wash protocol.
Figure 9. Example of a Wash Protocol
The procedures for specifying the steps in this and other wash protocols are
described on the following pages.
Opsys MW™ User's Guide
Creating a New Wash Protocol
For each strip, perform dispense three times.
For each strip, perform one aspiration cycle.
End the cycle.
®
plate.
45
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