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Chapter 4 Wash Protocols
Wash Protocol Operations
A wash protocol consists of a series of Purge, Move, Soak, Aspirate,
Dispense and Fill operations.
Purge, Move and Soak can be carried out in any sequence. Aspirate,
Dispense and Fill can only be carried out within a Move operation, and
there cannot be a Move within a Move.
Each of these operations is described below:
Operation
Purge
Move
Aspirate
Opsys MW™ User's Guide
Description
Dispenses fluid from the dispense wash pins while
the wash head is positioned over the purge tray
Performs Aspirate, Dispense, Fill and/or Soak
operations on specified strips of the Microplate
Removes the contents of a well by positioning the
wash pins at the aspiration height in the well and
aspirating the liquid from the wells
A sweep may also be performed during Aspirate.
Wash Protocol Operations
Note: Purge is usually
carried out at the beginning
of a wash protocol to rinse
the dispense wash pins and
remove air bubbles.
Note: The aspirate height
and sweep parameters for a
particular plate type are
specified during system
configuration (30).
If a sweep is specified during
configuration, the wash pins
are moved back and forth
during the aspiration cycle.
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