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ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment or data. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—EVOM™ Manual with a STX4 electrode.
INTRODUCTION
EVOM™ Manual is the WPI's newest instrument to measure Trans Epithelial Electrical
Resistance (TEER) measurement. The EVOM™ Manual has been re-engineered to
facilitate ease of accurate, repeatable measurements and transfer of data. You no
longer need to log data by hand. The resistance or voltage information is written to
a USB drive in a format (CSV) that can be opened by most spreadsheet programs on
either PC or Mac. The processor controlled instrument is more stable and accurate.
Included is a footswitch for quick hands free recording to the USB drive. The touch
screen interface is easy to use and setup for setting control wells in resistance or
voltage modes and measurements. The auto ranging resistance feature allows for fast
resistance measurements, and an overrange display feature eliminates false readings.
The EVOM™ Manual now has adjustable current levels in three fixed ranges with two
lower ranges for sensitive membranes and high resistance ranges up to 100K Ω.
Confluence of a cellular monolayer is determined by an increase or a plateau in tissue
resistance detected using the unique electronic circuit of the EVOM™ Manual and the
EVOM™ STX4 electrode. The EVOM™ Manual qualitatively measures cell monolayer
health and quantitatively measures cell confluence. The EVOM™ Manual produces
World Precision Instruments
EVOM™ Manual
3
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