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Summary of Contents for WPI STX4
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL STX4 Electrode with Replaceable Blades Compatible with EVOM Epithelial Volt/Ohm Meters www.wpiinc.com 031122...
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Page 3: Table Of Contents
Stable Repeatable Readings ..................... 5 Adjusting the Exposed Length of Electrode Blades ............6 Preparing to Take Measurements ..................7 MAINTENANCE ..........................9 Cleaning/Maintaining the STX4 Electrodes ................. 9 Disinfecting the STX4 Electrodes................... 9 Sterilizing the STX4 Electrodes ....................9 Storing the Electrodes ......................10 Replacing the Electrode Blades ...................11... - Page 4 World Precision Instruments...
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Page 5: About This Manual
This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—The STX4 electrode is designed for use with the EVOM3 meter. World Precision Instruments... -
Page 6: Introduction
(TEER)) of cultured epithelia (cellular layer) grown on permeable membranes in 12- and 24-well tissue culture plates. The STX4 can measure directly inside the well plates with 12 and 24 inserts. STX4 is ideal for the 24-well transwell format). -
Page 7: Notes And Warnings
STX4 • The electrode tip (active sensing area) of the STX4 is shorter than the STX2-PLUS tip. Therefore, it requires less liquid volumes than the STX2-PLUS to keep the electrode tips immersed and able to provide stable readouts. • Electrode blades are replaceable. Over a period of use (for example, months or... -
Page 8: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 27 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or customerservice@wpiinc.com. -
Page 9: Operating Instructions
Ease of Use The STX4 electrode design allows the electrode to hang vertically (Fig. 8, left) on top of the 24-well hanging transwells and does not need to be handheld. Insert the outer (longer) electrode into the basal access (well of the well plate) and the shorter one on top of the membrane. -
Page 10: Adjusting The Exposed Length Of Electrode Blades
The arrows highlight the coated sensing region. Align the electrode blades with the well inserts and position the STX4 over the well so that it rests securely in an upright position (Fig. 11, Fig. 12). (Images shown with a Corning 3470 24-well insert and plate.) For greater stability, rest the cable on the... -
Page 11: Preparing To Take Measurements
9.) Wipe the rest of the blades and the electrode body with a paper towel sprayed with alcohol. Position the blades of the STX4 electrode in the well plate. (See “Positioning Electrode in a Culture Cup” on page 5.) Let the electrode tips stay fully immersed in the media or buffer solution for five (5) minutes. - Page 12 After taking measurements, soak the electrode blade tips in 70% ethanol or isopropanol for 5-10 minutes, and then rinse them with distilled water and let them air dry before storage. (For details of the disinfection, see “Disinfecting the STX4 Electrodes” on page 9.) World Precision Instruments...
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Page 13: Maintenance
Disinfecting the STX4 Electrodes The STX4 electrode is resistant to most methods of low temperature (or room temperature) chemical disinfection. The electrode tips may be disinfected by immersing the tips in a disinfection solution. Wipe the rest of the electrode with a paper towel that has been sprayed with a cleaning solution. -
Page 14: Storing The Electrodes
CAUTION: Do NOT apply any heat or flame to any portion of the electrode or the electrode blades. Storing the Electrodes After a daily use, wash and store the electrodes dry away from light. Regular cleaning and maintenance is critical for proper functionality and for maintaining the functional life of the electrode blades. -
Page 15: Replacing The Electrode Blades
STX4 Replacing the Electrode Blades The electrode blades do not need to be replaced unless it is required. When abnormal reading instabilities are noticed, even after a recent cleaning with Tergazyme or Enzol and 5 minutes of equilibration of the electrode tip, replacement of the electrode blades is recommended. - Page 16 Outside Blade The white tab faces out. Fig. 21—The inside and outside electrode blades of STX4 electrode are different. Insert the new outside electrode first. Line up the electrode in the groove with the white tab facing out (Fig. 22), align the pins with the connector and gently push the electrode blade into place until it is properly seated (Fig.
- Page 17 STX4 padding through the center hole of the electrode (Fig. 24), align the pins with the connector and gently slide the pins into the connector until they are properly seated (Fig. 25). Avoid touching the coated portion of the tip.
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Page 18: Testing The Stx4 Electrode
No inserts or transwells are needed. Put the wet test fixture (WPI# 99776) provided by WPI on top of a 24-well plate over the filled wells (Fig. 28, Fig. 29). The fixture ensures consistent electrode positioning. - Page 19 STX4 Fig. 29— The test fixture is inserted all the way inside the wells of a 24-well plate. Fig. 30—The electrode is inserted so that the long blade is near the wall of the well and the short blade is near the center of the well.
- Page 20 If the resistance readings in the KCl solutions are out of range, then the electrode may need cleaning. See “Cleaning/ Maintaining the STX4 Electrodes” on page 9. Electrodes which are not being properly maintained may begin to show instabilities indicating the need for cleaning.
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Page 21: Accessories
STX4 Wet Test Fixture 7363-4 Enzol Enzymatic Detergent 13740 Alconox Powdered Precision Cleaner 504611 Rapicide OPA/28 High Level Disinfectant Fig. 31—Plug the STX4 into the 99675 cable to use the electrode with an EVOM2. The 99675 cable is sold separately. World Precision Instruments... -
Page 22: Troubleshooting
TROUBLESHOOTING Issue Possible Cause Solution The cable is heavy and can cause Lay the cable on the tabletop as close to the the electrode to tip over if too much electrode head as possible. of the cable is in the air. The electrode tips (sensing region) The electrodes tips (sensing region) require may have deposits of cell culture... - Page 23 ±1000 Ω is acceptable at the 100K range. Electrodes showing instability may require enzymatic cleaning. See “Cleaning/Maintaining the STX4 Electrodes” on page 9. NOTE: If you have a problem/issue that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or technicalsupport@wpiinc.com. World Precision Instruments...
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Page 24: Appendix A: Kcl Testing Mixtures
APPENDIX A: KCL TESTING MIXTURES To prepare solutions with different KCl concentrations, use the following table as a guide. Start by making a solution of 160 mM concentration. Molecular weight of KCl = 74.55 g/mol To make 100 mL of 160 mM KCl, add 1.193 gm KCl to 100 mL of distilled water. Add DI Water Results 50 mL of 160 mM KCl... -
Page 25: Appendix B: Compatible Inserts And Plates
STX4 APPENDIX B: COMPATIBLE INSERTS AND PLATES Membrane Growth Surface Membrane Corning Millipore Material Diameter (mm) Area (cm Pore Size (μm) 3470 0.33 3472 PITP01250 0.33 3413 PCF Insert 0.33 3415 PITP01250 0.33 PCF Insert 3421 0.33 3422 PIEP01250 0.33... - Page 26 Millicell Membrane Pore Size (μm) MCHT24H48 MCRP24H48 MCSP24H48 MCMP24H48 MCEP24H49 World Precision Instruments...
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Page 27: Frequently Asked Questions
STX4 FREQUENTLY ASKED QUESTIONS Do the STX4 electrode blade tips tend to darken over the period of use or during storage? Yes, it is a normal process. The STX4 electrode tips tend to darken (cosmetic), but the electrode or the electrode blade functionally remains unaffected. - Page 28 Why am I getting dashes as reading on EVOM3, even if I have the STX4 electrode inside the sample well? An electrode in air or partially immersed in the liquid can show dashes since it records unstable read outs. The electrode tip portion (sensing region) must stay fully immersed.
- Page 29 STX4 Why are the readings fluctuating while using STX4 and what should I do? Fig. 34—Liquid on the metal surface (orange arrow) of the electrode can contribute to reading fluctuations. Liquid in contact with the metal surface (Fig. 34, see orange arrow) can lead to reading fluctuations.
- Page 30 Can you suggest some experimental parameters that can be controlled to obtain more consistent TEER results? • Temperature is known to affect TEER values. We recommend that you maintain a consistent sample temperature to obtain consistent values. We suggest taking the well plate with inserts containing cells out of the incubator and letting the plate stabilize at room temperature inside the laminar flow hood for 15-20 minutes before taking measurements.
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Page 31: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 32 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
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