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Summary of Contents for WPI EVOM3
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL EVOM3 Epithelial Volt/Ohm Meter Serial No._____________________ www.wpiinc.com 063020...
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Page 3: Table Of Contents
Resistance ........................... 1 Voltage ............................1 TEER Calculations ........................2 ABOUT THIS MANUAL ........................3 INTRODUCTION ..........................3 EVOM3 Features ........................4 enefit ......................... 4 STX2-PLUS Features ......................... 5 enefit ........................5 Notes and Warnings ......................... 5 Tips and Advice .......................... 6 PARTS LIST ............................ - Page 4 MAINTENANCE ..........................26 Cleaning/Maintaining the STX2-PLUS Electrodes............26 Disinfecting the STX2-PLUS Electrodes ................26 Sterilizing the STX2-PLUS Electrode ...................27 Rechloriding the Electrodes ....................27 Storing the Electrodes ......................27 Testing the STX2-PLUS Electrodes ..................28 ACCESSORIES..........................28 TROUBLESHOOTING ........................29 SPECIFICATIONS ..........................34 APPENDIX A: KCL TESTING MIXTURES ..................35 APPENDIX B: FILE FORMATS ......................35 Resistance (*.CSV) ........................35 Voltage (*.csv) ..........................35...
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Page 5: Quick Start
Press Return to navigate back to the main menu. Insert a USB thumb drive into the USB port on the side of the EVOM3 and begin measuring by pressing the foot switch or touching the Store button on the screen to store and advance to the next weel. -
Page 6: Teer Calculations
Press Return to navigate back to the main menu. Insert a USB thumb drive into the USB port on the side of the EVOM3 and begin measuring by pressing the foot switch or touching the Store button on the screen. -
Page 7: About This Manual
NOTES and TIPS contain helpful information. Fig. 1—EVOM3 INTRODUCTION EVOM3 is the WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER) measurement. The EVOM3 has been re-engineered to facilitate ease of accurate, repeatable measurements and transfer of data. You no longer need to log data by hand. -
Page 8: Evom3 Features
DC currents. The EVOM3 uses low current and voltages and i de igned n n de t ti e te ting... -
Page 9: Stx2-Plus Features
The chager may be used to power the EVOM3 for normal and long-term use. NOTE: In the higher resistance ranges (50K -100K ) there may be a slight instability added from the noise of the power supply, in that case unplug it during those measurements. -
Page 10: Tips And Advice
EVOM3 to read a lower resistance. Instead of the current going through the membrane, the electricity is going around the membrane. Electricity will take the path of least resistance and the EVOM3 will measure that as a lower resistance. -
Page 11: Parts List
In the STX2-PLUS manual is a KCl molarity test. This test will demostrate electode functionality. Charging the EVOM3 The EVOM3 battery may also be charged with a Mini-B USB cable P/N 803026 (not supplied). Parts List After unpacking, verify that there is no visible damage to the sensor. Verify that all... -
Page 12: Unpacking
“Claims and Returns” on page 47 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or customerservice@wpiinc.com. Returns: Do not return any goods to WPI without obtaining prior approval (RMA... -
Page 13: Instrument Description
EVOM3 INSTRUMENT DESCRIPTION EVOM3 Meter Fig. 3—USB port, Electrode connection Fig. 4—Foot switch, Future expansion, reset, power connection, power switch STX2-PLUS Electrodes Fig. 5—STX2-PLUS electrode World Precision Instruments... -
Page 14: Theory Of Operation
Slide the cable on the table toward the plate and electrode to prevent tipping. Theory of Operation The EVOM3 system uses a four electrode sensor, two for current sourcing and two for voltage measurement. The EVOM3 passes a known constant current through the membrane on two electrodes and measures the voltage needed to pass that current on the other two electrodes and computes resistance using Ohms law. -
Page 15: Setup Page
EVOM3 Setup Page Press Setup on the main screen to open the Setup page. Fig. 7—Setup page Select Plate – Select the number of wells and the sampling pattern. Mode Units – Choose the Units for sampling. (Options include Millivolts, Volts, Ohms and K Ohms.) -
Page 16: Mode Units
Press Return to save the setup. When you press Preview from the main screen ill ee the late e ie file li e the am le Fig. 9—(Left) 6-well plate preview Fig. 10—(Right) 12-well plate preview Fig. 11—(Left) 24-well plate preview Fig. -
Page 17: Blank Handling Page
EVOM3 Choose your units. Options include Millivolts, Volts, Ohms and K Ohms. If you choose ohms, then you must also select an Ohms Range. Fixed Current Variable Current 10 K 10 µA Auto 2 µA to 10 µA 50 K 4 µA... -
Page 18: Probe Null
Press Date Format. Choose your preferred format and press Return. Fig. 18—(Left) Set the date and press Return Fig. 19—(Right) Choose the date format and press Return Press Set Time. Set the appropriate time and press Return. Press Time Format. Choose 12 hour or 24 hour and press Return. Fig. -
Page 19: Storing Data
file i saved. Select the Auto Index radio button to enable the Store Prefix and Auto Index buttons. Press Auto Index if you want the EVOM3 to automatically number the new data file entiall t inde ing hange the name t a n me i... -
Page 20: Viewing Data In Microsoft Excel Or Other Spreadsheet Program
Viewing Data in Microsoft Excel or Other Spreadsheet Program Remove the USB thumb drive from the EVOM3 and plug it into a free USB slot in your computer. en the d i e and l file named PlateX.CSV. If your computer has Microsoft... -
Page 21: Usb Testing
USB thumb drive can be used, otherwise an error is displayed. Fig. 27—(Left) The USB thumb drive passed the test and is suitable for use with the EVOM3. Fig. 28—(Right) The USB thumb drive is not suitable for use with the EVOM3. -
Page 22: System Messages
SYSTEM MESSAGES Here are some warning messages you may see. Last Cell Warning Fig. 29—After the last cell measurement is taken, this warning appears. hi i the indi ati n that the la t ell in the late ha een ea hed and the file need to be stored. - Page 23 EVOM3 When the battery power level falls to 1%, a warning message appears. Save your data file he data i a ed t a a file named Fig. 31—When the battery level falls to 1%, you are asked to save your data file.
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Page 24: Reloading A Backup File After Power Failure
Reloading a Backup File after Power Failure When power is restored a green message appears. Press OK. Fig. 36—(Left) Power level is restored when the unit is plugged into a power source. Cycle power on the EVOM3 t ning it and n again hi... -
Page 25: Operations
Press the OK in the upper left of the octagonal icon on the main display page to short the electrodes. Verify that the EVOM3 ead Ω To return to normal EVOM3 operation, unplug the test resistor and press the button (uppper left). Testing the EVOM3 Meter (Voltage) On the main display, select the Setup page. -
Page 26: Making Resistance Measurements
Making Resistance Measurements Power on the EVOM3 using the power switch on the rear panel. a filename the than the de a lt ( late ) i i ed, h e hethe efi and a t inde ing a ni e file name... - Page 27 • Press Return to navigate back to the main menu. Insert a USB drive into the USB port on the side of the EVOM3 and begin measuring by pressing the foot switch or touching the Store button on the screen.
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Page 28: Making Voltage Measurements
Making Voltage Measurements Power on the EVOM3 using the power switch on the rear panel. a filename the than the de a lt ( late ) i i ed • Press and hold the Store button on the main screen for 2 seconds or press... - Page 29 • Press Return to navigate back to the main menu. Insert a USB drive into the USB port on the side of the EVOM3 and begin measuring by pressing the foot switch or touching the Store button on the screen.
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Page 30: Maintenance
MAINTENANCE Electrodes must be properly cleaned, sterilized and stored. CAUTION n t ame ele t de ill a e them t melt Cleaning/Maintaining the STX2-PLUS Electrodes IMPORTANT! With the use of DMEM, the electrode surface will become coated with protein or other foreign materials. This build-up, or contamination, will degrade the performance of the system. -
Page 31: Sterilizing The Stx2-Plus Electrode
EVOM3 CAUTION: Ammonia is NOT recommended, because silver chloride dissolves in ammonia. Sterilizing the STX2-PLUS Electrode The STX2-PLUS electrodes are non-sterile as supplied. Acceptable low temperature steril- ization methods for the electrodes include gamma irradiation and ethylene oxide gas (ETO). -
Page 32: Testing The Stx2-Plus Electrodes
ACCESSORIES WPI Part # Description EVOM3 ACCESSORIES & REPLACEMENT PARTS 99672 EVOM3 legacy electrode Adaptor to use EVOM3 with EVOM2 electrode 99673 EVOM3 ali ati n Kit Ω e t Re i t 99675 EVOM2 Electrode adaptor to use EVOM2 with EVOM3 electrodes... -
Page 33: Troubleshooting
5 mm tall, 3 mm wide and 1 mm in thickness. Each probe has an inner (voltage) and an outer (current) electrode. STX3 is an alternative electrode to the STX2 that can be used with the EVOM3 for m the STX2 in that the vertical distance shallower inserts (5–9 mm inserts). - Page 34 Defective charger The EVOM3 charge socket can be tested with a USB to Mini-B cable plugged into a computer’s USB port (WPI #803026). If the charging LED lights up, then a new char- ger is needed (WPI #803025).
- Page 35 EVOM3 Issue Possible Cause Solution Electrode not The Ag/AgCl electrodes (inner voltage measurement ili ated electrodes) on the probe may become polarized due to an uneven distribution of ions between the electrode pair. This ionic charge can be neutralized by immersing the probe into 0.1M KCL for 24 hours with the probe elec-...
- Page 36 Issue Possible Cause Solution Contamination on U e the Ω te t e i t e i that the mete i electrodes e tl he mete di la ld h Ω See "Instrument Diagnostics and Calibration" on page 21. If a negative value exists only when using the STX electrode, inspect and test the electrode for the possible formation of an insulative coating of foreign material that electrically blocks the current and voltage electrodes.
- Page 37 NOTE ha e a lem i e ith that all t ide the definiti n troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. i t Reading that ntin l in ea e de ea e a ignifi ant al e (eithe voltage or resistance) over time.
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Page 38: Specifications
Charge Current ........................200 mA Power Consumption ......................~250 mA e tifi ati n ............................ CE Firmware ........................Upgradeable* *NOTE: A USB to Mini-B cable (WPI #803026) i i ed al ng ith tl ade t a e and the image a e e i ed t g ade the fi... -
Page 39: Appendix A: Kcl Testing Mixtures
100 mL 20 mM KCl 25 mL of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B: FILE FORMATS Resistance (*.CSV) Date 03/12/2020 13:11:56 Well Plate 24 EVOM3 Version 1.0 Units: OHMS Direction 1 Resistance Scale 50k Ohms 19015 19015 19015... -
Page 40: Appendix C: Continuous Recording Via Usb (Python) On A Pc
External Data Output program. To use these programs: Connect the EVOM3 to the USB port of the PC. The active remote connection port for the EVOM3 service port is in the back of the unit as a USB-B connector [ marked in red]. - Page 41 Connect the EVOM3 to the USB port of the PC. The active remote connection port for the EVOM3 service port is in the back of the unit as a USB-B connector [ marked in red]. Fig. 51—Plug the USB-B (red) connector into the port on the back of the EVOM3.
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Page 42: Sample Csv Recording
At a command line or from python type this command : python WPI_ EVOMCNX_3.E.py and press enter. If you are not running Python from the directory where WPI_EVOMCNX_3.E.py was extracted, you will have to change directory to that location. nte the filename t File ( e in a filename and add the... -
Page 43: Appendix D: 24 Mm Diameter (6-Well) Inserts
Note that the resistance readings from 24 mm diameter tissue culture inserts (6- well plates) obtained by using WPI’s STX series of electrodes will be 1.8 to 2.2 times higher than that obtained using the ENDOHM-24G, because the STX electrode cannot deliver a uniform current density over the relatively large membrane through the small gap between the membrane and the bottom of the well. - Page 44 EndOhm chamber, STX2-PLUS or the STX100 electrodes instead of standard STX2 or STX3 electrodes. In some cases where the insert height is shorter than 9 mm the STX3 must be used. The 99672 EVOM3 adaptor must be used for the STX3, STX2, STX100 electrodes.
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Page 45: Appendix F: Resistance Calculations
Ω Ω Ω true tissue Resistance Value of the “Blank” Insert When using an EVOM3 with an STX2-PLUS to measure a blank insert, the resistance al e i t i all et een Ω, de ending n the e ifi... -
Page 46: Unit Area Resistance
NOTE: Resistance readings for 24 mm or larger diameter inserts obtained by using the EVOM3 with the STX electrodes should not be converted to unit area resistance. The EndOhm is recommended for these larger inserts. The unit area resistance is... -
Page 47: Teer Calculations
• 24 well plate 6.5 mm inserts) 0.3316 cm • 96 well plate (4.3 mm inserts) 0.145 cm APPENDIX G: FREQUENTLY ASKED QUESTIONS Will the EVOM3 work with the EndOhms? Answer: Yes, but an 99916 ada t i ed (... -
Page 48: Declaration Of Conformity
175 Sarasota Center Boulevard Sarasota, FL 34240-9258 USA As the manufacture/distributor of the apparatus listed, declare under sole responsibility that the product(s): WPI PN: EVOM3 To which this declaration relates is/are in conformity with the following standards or other normative documents: Safety:... - Page 49 EVOM3 World Precision Instruments...
- Page 50 World Precision Instruments...
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Page 51: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 52 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany fing t eide F ied e g ( e en), e man mail China &...
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