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Summary of Contents for WPI EVOM
- Page 1 INSTRUCTION MANUAL EVM-MT-03-02 EVOM™ Manual for TEER Measurement with Secure Data Transfer Capability www.wpiinc.com 040325 040325...
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Page 3: Table Of Contents
Mode Units ..........................13 Blank Handling Page ......................14 Setting the Date and Time ....................14 Probe Null ..........................15 Storing Data ..........................15 Starting the EVOM™ Companion Application ..............20 Viewing Data in a Spreadsheet Program ................22 USB Testing ..........................24 OPERATIONS ..........................25 Instrument Diagnostics and Calibration................25 Taking Measurements ......................26... - Page 4 APPENDIX D: RESISTANCE CALCULATIONS ................46 Resistance ..........................46 Unit Area Resistance ......................46 TEER Calculations ........................47 APPENDIX E: FREQUENTLY ASKED QUESTIONS ..............48 APPENDIX F: INSTALLING THE EVOM™ COMPANION APPLICATION ....... 49 DECLARATION OF CONFORMITY ....................50 WARRANTY .............................51 Claims and Returns ........................51 Repairs ............................51...
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Page 5: Quick Start For Taking Measurement
• If you selected USB Save, you need to insert the USB flash drive into the USB port on the side of the EVOM™ Manual. See "Storing Data on the USB Flash Drive" on page 17. • If you selected APP Save, need to make sure the EVOM™ Manual is properly connected to the computer using the USB cable included and verify that the Companion Application is running. -
Page 6: Teer Calculations
Next returns you to the main screen so you can take measurements of the next plate which starts from a clear state. NOTE: If you are on plate 3, the Next button does not appear, because EVOM™ Manual’s memory only holds data from three plates. -
Page 7: About This Manual
This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. Please read these carefully. NOTES and TIPS contain helpful information. Fig. 1—EVOM™ Manual with a STX4 electrode. World Precision Instruments... -
Page 8: Introduction
The auto ranging resistance feature allows for fast resistance measurements, and an overrange display feature ensures no false readings. The EVOM™ Manual now has adjustable current levels in three fixed ranges with two lower ranges for sensitive membranes and high resistance ranges up to 100KΩ. -
Page 9: Evom™ Manual Benefits
Use of the charger: The EVOM™ Manual's Li-ion battery lasts 8 hours on its own. The charging time is 5.5 hours with the unit powered off. The EVOM™ Manual charger should not be plugged in all the time. The charger may be used to power the EVOM™ Manual for normal and long-term use. -
Page 10: Tips And Advice
Press Cancel and try again. The Store function cannot be used on an unrecognized drive. EVOM™ Manual should respond with “File saved” within 15 seconds. If “Saving file...” is still seen after 20 seconds, press the Return key and use another USB flash drive. -
Page 11: Parts List
EVOM™ Manual to read a lower resistance. Instead of the current going through the membrane, the electricity is going around the membrane. Electricity will take the path of least resistance and the EVOM™ Manual will measure that as a lower resistance. -
Page 12: Unpacking
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 51 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or customerservice@wpiinc.com. -
Page 13: Instrument Description
The EVOM™ Manual meter is compact and portable, and setup is quick. USB Port for Flash Drive Electrode Port Fig. 3—The ports on the side of the unit let you connect a USB flash drive and the EVOM™ electrode. The side panel connection ports include: •... -
Page 14: Theory Of Operation
Use the included USB cable (WPI #503535) to connect the EVOM™ Manual to your PC. The type A end plugs into a port on the computer, and the type B end plugs into this port on the EVOM™ Manual. -
Page 15: System Setup
File name, defined in the Store Options menu Fig. 5—The main EVOM™ Manual display. A negative resistance number will appear in red text. This can be caused by a faulty electrode in media, (see "System Messages" on page 32) or a blank setting that is over the measurement (see "Blank Handling Page"... -
Page 16: Plate Selection Page And Plate Preview
• Short Electrodes – Shorts V and is used to nullify the galvanic charge in the electrodes when the electrodes are placed in a saline solution for 4–8 hours to remove the charge. See also "Probe Null" on page 15. •... -
Page 17: Mode Units
EVOM™ Manual Fig. 12—96-well plate preview. (Press scroll to see the additional wells.) NOTE: An out of resistance range displays ---- (5 dashes) on the screen. Adjust the range to correct the error. A saved well under preview will show a space (“ “) if the display was recorded out of range. -
Page 18: Blank Handling Page
Blank Handling Page Press Blank Handling on the Setup page to subtract the control well blank from the current resistance measurement. Fig. 14—(Left) Blank Handling window. Fig. 15—(Right) The main screen shows the offset underneath the reading. Insert the probe into an empty well filled with media. Press Reading to make a measurement. -
Page 19: Probe Null
Data may be saved to a USB flash drive plugged into the USB port on the side of the unit. • Data may also be stored on a PC with the use of the EVOM™ Companion Application. The EVOM™ Manual can store up to three plates of data without World Precision Instruments... - Page 20 USB save stores the files to the USB flash drive. Make sure the USB flash drive is installed. • APP save stores the files on a connected PC using the EVOM™ Companion Application. Be sure to properly connect the EVOM™ Manual to the PC and start the Companion Application before storing measurements.
- Page 21 For APP Save, see "Storing Data on a Connected PC" on page 19. NOTE: Since there are three plates that can be held in the EVOM™ Manual’s memory before data is exported to a storage device, there could be as many files as the number of plates analyzed kept in the memory.
- Page 22 The note above the Setup button on the main screen indicates which plate data is being collected (Fig. 5). NOTE: If you are on plate 3, the Next button does not appear, because EVOM™ Manual’s memory only holds data from three plates.
- Page 23 When the plate data has been saved, a confirmation message appears (Fig. 31). Press the Done button to return to the main screen. NOTE: EVOM™ Manual has a time out in case the PC is not connected or the EVOM™ World Precision Instruments...
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Page 24: Starting The Evom™ Companion Application
PC and the EVOM™ Companion Application is running on the PC. Starting the EVOM™ Companion Application To be able to save files to a PC, the EVOM™ Companion Application must be running on the PC. To install the EVOM™ Companion Application, see "Appendix F: Installing the EVOM™... - Page 25 EVOM™ Manual Fig. 35—The WPI icon appears in the taskbar when the program is running. Right click to pin it to the taskbar for future use. The EVOM™ Companion Application opens (Fig. 36). Fig. 36—The application opens and asks you to select a data storage folder.
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Page 26: Viewing Data In A Spreadsheet Program
Viewing Data in a Spreadsheet Program If you stored the data on the USB flash drive, remove it from the EVOM™ Manual and plug it into a free USB slot in your computer. Using Windows® Explorer, navigate to the USB flash drive directory. - Page 27 Sample data file shows: • Date, time, plate size • EVOM™ Manual firmware revision • Measurement mode with a blank or a voltage offset (PD indicates Potential Difference and is seen when taking voltage measurements.) •...
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Page 28: Usb Testing
If the drive is usable a message indicates that the USB flash drive can be used (Fig. 42), otherwise an error displays (Fig. 43). Fig. 42—(Left) The USB flash drive passed the test and is suitable for use with the EVOM™ Manual. -
Page 29: Operations
• If it is new, test the USB flash drive. See "USB Testing" on page 24. • Validate the EVOM™ Manual using 1000 Ω test resistor or calibrate the EVOM™ Manual. Instrument Diagnostics and Calibration WPI recommends that EVOM™ Manual be put through the diagnostics described below before using it for the first time and then periodically thereafter. -
Page 30: Taking Measurements
Taking Measurements Configuring Data Storage Options Power on the EVOM™ Manual using the power switch on the rear panel. To configure your data file storage settings, hold the Store button on the main screen for 2 seconds or press Store Screen on the Setup screen to access the Store Options screen. - Page 31 • If you selected USB Save, you need to insert the USB flash drive into the USB port on the side of the EVOM™ Manual. See "Storing Data on the USB Flash Drive" on page 17. • If you selected APP Save, you need to make sure the EVOM™ Manual is properly connected to the computer using the USB cable included and verify that the Companion Application is running.
- Page 32 Fig. 47—Select the mode. For resistance measurements, choose OHMS or K OHMS. Then select the range. Press the Calibrate button to calibrate the electrode. (This uses a 10 KΩ 0.1% resistor). See "Instrument Diagnostics and Calibration" on page 25. To subtract a blank well (including the fluid and electrode resistances) press the Blank Handling button.
- Page 33 EVOM™ Manual Press the Mode Units button and choose either Millivolts or Volts for making voltage measurements (Fig. 51). Press the Return button to go back to plate settings screen (Fig. 50). Fig. 51—(Right) Select the mode. For voltage measurements, choose Millivolts or Volts.
- Page 34 The note above the Setup button on the main screen indicates which plate data is being collected (Fig. 5). NOTE: If you are on plate 3, the Next button does not appear, because EVOM™ Manual’s memory only holds data from three plates.
- Page 35 Fig. 59—When the USB flash drive is not plugged in or not recognized, a warning message appears. NOTE: EVOM™ Manual should respond with “Plate data saved to USB” within 15 seconds. If “Saving file...” is still seen after 20 seconds, press the Back button and use another USB flash drive.
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Page 36: System Messages
Last Cell Warning When you are taking measurements, and you reach the last cell, a message appears. Fig. 60—(Left) After the last cell measurement is taken and you are saving to the EVOM™ Companion Application, this message appears. Fig. 61—(Right) If you are saving to the USB drive, the message changes a little. -
Page 37: Reloading A Backup File After Power Failure
Fig. 68—(Left) Power level is restored when the unit is plugged into a power source. Cycle power on the EVOM™ Manual by turning it off and on again by sliding the power switch on the back panel. This automatically loads the backup files which... - Page 38 were saved when the power failed. The backup files are deleted from the USB flash drive. You will see messages that indicate which plate or plates are being restored (Fig. 69).Then, a success message appears (Fig. 70). Press the OK button to preview the data.
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Page 39: Accessories
EVOM™ STX HTS Electrode for TEER in Corning Biocoat/Falcon 24 EVM-EL-03-02-04 EVOM™ STX HTS Electrode for TEER in Corning Costar 24 EVM-EL-03-03-01 EVOM™ STX4 Electrode with Removable Blades for TEER in 6 mm Inserts EVM-AC-03-01-01 EVOM™ Electrode Blades for the EVOM™ STX4 for TEER, 3 pairs EVM-AC-03-03 EVOM™... -
Page 40: Troubleshooting
TROUBLESHOOTING EVOM™ Manual Meter: Most of the time, system problems are related to the electrode, not the meter itself. Some issues you may encounter include: • UNSTABLE OR HIGH READING: If the electrode fails, the most common symptom is an unstable or unusually high reading. - Page 41 EVOM™ Manual Issue Possible Cause Solution Electrodes are Clean the electrodes as per the instruction manual for heavily charged the electrode. or dirty Electrode too Move the system to a different area away from sources close to strong of electromagnetic fields. Electromagnetic field sources...
- Page 42 Issue Possible Cause Solution Cell culture or If the cell culture has been given sufficient time to achieve media problem confluence, and the reading is stable but significantly lower than expected, then the problem is probably related to the cell culture. Electrode failure will not generally cause a lower than expected yet stable reading.
- Page 43 NOTE: The EVOM™ Manual will read only about 5% above 100K Ω. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or wpi-ts@wpiinc.com. *Drift–Readings that continuously increase or decreases a significant value (either voltage or resistance) over time.
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Page 44: Specifications
Power Consumption ......................~250 mA Certifications ............................ CE Firmware ........................Upgradeable* *NOTE: A USB to Mini-B cable (WPI #803026) is required along with PC bootloader software and the image are required to upgrade the firmware. **Not user serviceable. Contact WPI for repair or replacement. -
Page 45: Appendix A: File Formats
EVOM™ Manual APPENDIX A: FILE FORMATS Resistance (*.CSV) Date 03/12/2020 13:11:56 Well Plate 24 EVOM™ Manual Version 2.0 MODE OHMS offset 999.8 Units: OHMS Direction 1 Resistance Scale 50k Ohms 19015 19015 19015 19015 19015 19015 19016 19016 19016 19016... -
Page 46: Appendix B: Continuous Recording Via Usb (Python) On A Pc
External Data Output program. To use these programs: Connect the EVOM™ Manual to the USB port of the PC. The active remote connection port for the EVOM™ Manual service port is in the back of the unit as a USB-B connector [ marked in red]. - Page 47 Extract the files to a WPI directory or a directory of your choice. Connect the EVOM™ Manual to the USB port of the PC. The active remote connection port for the EVOM™ Manual service port is in the back of the unit as a USB-B connector [ marked in red].
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Page 48: Sample Csv Recording
Open Python or a command window on your system. At a command line or from python type this command : python WPI_ EVOMCNX_3.E.py and press enter. If you are not running Python from the directory where WPI_EVOMCNX_3.E.py was extracted, you will have to change directory to that location. -
Page 49: Appendix C: Improving The Accuracy And Repeatability Of The System
STX2-PLUS electrode. It has removable blades. Fig. 73 demonstrates how the EVOM™ electrodes measures TEER. With a fixed centered electrode geometry of the EndOhm, the variation of readings on the same sample is 1-2 Ω, as compared to 5% of the total reading using the STX2 electrodes. -
Page 50: Appendix D: Resistance Calculations
APPENDIX D: RESISTANCE CALCULATIONS Resistance The value of the blank (control well) always adds to the total resistance measured across a tissue culture membrane. The blank resistance must be measured and then subtracted from the resistance reading across the tissue in order to obtain the true tissue resistance. -
Page 51: Teer Calculations
EVOM™ Manual = 670 Ω * 0.331 cm = 221.77 Ω-cm 221.77 Ω is the resistance of a unit area of 1 cm (TEER). The larger the membrane, the lower the resistance. The dimension is Ω-cm , not Ω / cm TEER Calculations TEER is a normalized value of resistance per 1 square centimeter of unit area. -
Page 52: Appendix E: Frequently Asked Questions
APPENDIX E: FREQUENTLY ASKED QUESTIONS When do researchers want to use the blank function? Answer: The blank function allows researchers to subtract out resistance measurement that is not caused by the cell layer. This could be a reading affected by the electrodes or fluid resistances or other factors. Normally, you would measure a blank insert with the same volumes as the insert and keep subtracting that blank value from the samples. -
Page 53: Appendix F: Installing The Evom™ Companion Application
Fig. 75—(Right) Select the WPI application from the list that display. TIP: After the program has launched, right click on the WPI icon (Fig. 76) in the taskbar and select Pin to taskbar. Then, you can double click the taskbar icon to load the program in the future. -
Page 54: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 55: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 56 W OR LD P RE C I SI O N I NSTR U M E N TS...
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