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Summary of Contents for WPI EVOM Manual
- Page 1 INSTRUCTION MANUAL EVM-MT-03-01 EVOM™ Manual for TEER Measurement www.wpiinc.com 070924 070924...
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Page 3: Table Of Contents
EVOM™ Manual CONTENTS QUICK START ........................... 1 Resistance ........................... 1 Voltage ............................1 TEER Calculations ........................2 ABOUT THIS MANUAL ........................3 INTRODUCTION ..........................3 EVOM™ Manual Features ......................4 EVOM™ Manual Benefits ......................4 Notes and Warnings ......................... 5 Tips and Advice .......................... - Page 4 APPENDIX A: FILE FORMATS.......................31 Resistance (*.CSV) ........................31 Voltage (*.csv) ..........................31 APPENDIX B: CONTINUOUS RECORDING VIA USB (PYTHON) ON A PC ......31 Running the External Data Connection Output Program (PC, Mac, Linux) ....32 Sample CSV recording ......................34 APPENDIX C: IMPROVING THE ACCURACY AND REPEATABILITY OF THE SYSTEM ..35 APPENDIX D: RESISTANCE CALCULATIONS ................36 Resistance ..........................36 Unit Area Resistance ......................36...
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Page 5: Quick Start
EVOM™ Manual QUICK START Resistance Power on the EVOM™ Manual using the power switch on the rear panel. If a filename other than the default (plate1) is required: • Press and hold the Store button for 2 seconds to enter the Store Option menu. •... -
Page 6: Teer Calculations
• Select Filename to enter a new name. • Press Clear. • Use the on screen keypad to enter a new name. • Press Enter. NOTE: Auto indexing changes the name to a numeric sequence formatted with the name, a prefix and a sequential number (name_prefixN). Press Setup to access the Setup menu. -
Page 7: About This Manual
Fig. 1—EVOM™ Manual with a STX4 electrode. INTRODUCTION EVOM™ Manual is the WPI’s newest instrument to measure Trans Epithelial Electrical Resistance (TEER) measurement. The EVOM™ Manual has been re-engineered to facilitate ease of accurate, repeatable measurements and transfer of data. You no longer need to log data by hand. -
Page 8: Evom™ Manual Features
a low AC current that avoids electrode metal deposits and adverse effects on tissues which can otherwise be caused by higher DC currents. The EVOM™ Manual uses low current and voltages and is designed for non-destructive testing for epithelial monolayer confluence in cell cultures. In addition, resistance readings are unaffected by membrane capacitance or membrane voltage. -
Page 9: Notes And Warnings
USB Drives: WPI has supplied a tested USB thumb drive. Most drives of 32 Gb (USB2.0) or smaller sizes have been tested. CAUTION: Not all USB drives work on the EVOM™ Manual. - Page 10 Zeroing the blank handling value Select Setup. Then, press Short Electrodes. Press the Blank Handling button. Set the reading to zero. Return to the Setup page and open the electrodes to remove the short. Zeroing the probe null offset Select Setup. Then, press Short Electrodes.
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Page 11: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 39 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or customerservice@wpiinc.com. -
Page 12: Instrument Description
INSTRUMENT DESCRIPTION EVOM™ Manual Meter Fig. 3—USB port, Electrode connection Fig. 4—Foot switch, Future expansion, reset, power connection, power switch Theory of Operation The EVOM™ Manual system uses an electrode assembly made of four sub- electrodes, two for current sourcing and two for voltage measurement. The EVOM™... -
Page 13: Main Display
EVOM™ Manual Main Display Touch to short electrodes Current cell in plate Battery indicator Range (V, mV, Ω, KΩ) Press to record and index to the next well. Press and hold to open the storage options menu Preview the plate by its layout File name, defined in the storage options menu... -
Page 14: Plate Selection Page And Plate Preview
Date/Time – Select the Date and Time formats for your data files. Calibrate – Calibrate the electrode. (This uses a 10K Ω 0.1% resistor.) See "Instrument Diagnostics and Calibration" on page 20. Probe Null – Neutralize the offset charge on V Store Screen–... -
Page 15: Mode Units
EVOM™ Manual Fig. 12—96-well plate preview. (Press scroll to see the additional wells.) NOTE: An out of resistance range will display ---- (5 dashes) on the screen. Adjust the range to correct the error. A saved well under preview will show a space (“ “) if the display was recorded out of range. -
Page 16: Blank Handling Page
Blank Handling Page Press Blank Handling on the Setup page to subtract the control well blank from the current resistance measurement. Fig. 14—(Left) Blank Handling window. Fig. 15—(Right) The main screen shows the offset underneath the reading. Insert the probe into an empty well filled with media. Press Reading to make a measurement. -
Page 17: Probe Null
EVOM™ Manual Press Set Time. Set the appropriate time and press Return. Press Time Format. Choose 12 hour or 24 hour and press Return. Fig. 19—(Left) Set the time and press Return Fig. 20—(Right) Choose the time format and press Return Probe Null Press Probe Null on the Setup page to access the NULL Probe Process screen. -
Page 18: Storing Data
Storing Data Press and hold the Store button on the main screen for 2 seconds or press Store Screen on the Setting screen to configure your stored data files. Fig. 23—Use the Store Options screen to save a data file. Press New Plate to clear any recorded data on the preview screen and from memory. -
Page 19: Viewing Data In Microsoft Excel Or Other Spreadsheet Program
EVOM™ Manual Viewing Data in Microsoft Excel or Other Spreadsheet Program Remove the USB thumb drive from the EVOM™ Manual and plug it into a free USB slot in your computer. Open the drive and look for files named PlateX.CSV. If your computer has Microsoft Excel or another spreadsheet program the file ®... -
Page 20: Usb Testing
USB Testing From the Store Options page you can test a USB thumb drive to verify that it can be used. This drive must be formatted as FAT32. Insert the drive to see if it is recognized by the EVOM™ Manual. From the main page, press the Settings button. -
Page 21: System Messages
EVOM™ Manual SYSTEM MESSAGES Here are some warning messages you may see. Last Cell Warning Fig. 28—After the last cell measurement is taken, this warning appears. This is the indication that the last well in the plate has been reached and the file needs to be stored. - Page 22 When the battery power level falls to 1%, a warning message appears. Save your data file. The data is saved to a backup file named PWRFAILDATA.TXT. Saving Data for Power down... Save data to your file now! Fig. 30—When the battery level falls to 1%, you are asked to save your data file. When the battery level falls to 0%, the system begins a 60 second countdown before powering off.
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Page 23: Reloading A Backup File After Power Failure
EVOM™ Manual Reloading a Backup File after Power Failure When power is restored a green message appears. Press OK. Fig. 34—(Left) Power level is restored when the unit is plugged into a power source. Cycle power on the EVOM™ Manual by turning it off and on again. This will automatically load the backup file which was saved when the power failed. -
Page 24: Operations
EVOM™ Manual. Instrument Diagnostics and Calibration WPI recommends that EVOM™ Manual be put through the diagnostics described below before using it for the first time and then periodically thereafter. If there is a concern that the meter or the electrode is not functioning properly, the following protocols may be used to confirm the EVOM™... -
Page 25: Making Resistance Measurements
EVOM™ Manual Test Resistor “Calibration Kit” The 99673 test resistor is supplied to verify the calibration. Making Resistance Measurements Power on the EVOM™ Manual using the power switch on the rear panel. If a filename other than the default (plate1) is required, choose whether you will use a prefix and auto-indexing or a unique file name: •... - Page 26 Fig. 38—Setup page Select Plate–Choose the plate size. • • Select Mode Units – Choose OHMS or KOHMS for making resistance measurements. Select the resistance range (auto or 10,000 ohms). • Calibrate–Press Calibrate to calibrate the electrode. (This uses a 10 KΩ 0.1% resistor).
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Page 27: Making Voltage Measurements
EVOM™ Manual should respond with “File saved” within 15 seconds. If “Saving file...” is still seen after 20 seconds, press the Return key and use another USB drive. Once a file has been saved, open the file on a computer to verify the contents. NOTE: The file saving routine can take up to 15 seconds to complete. - Page 28 Press Setup to access the Setup menu. Fig. 44—Setup page • Select Plate–Choose the plate size. • Select Mode Units – Choose volts or millivolts (the normal mode is millivolts). • Electrode Equilibration – Zero the electrodes' galvanic charge in saline. •...
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Page 29: Accessories
NOTE: The file saving routine can take up to 15 seconds to complete. See the USB cautions on page 5. Remove the USB thumb drive to transfer the data file to your computer as a CSV file (Excel). ACCESSORIES WPI Part # Description EVM-EL-03-01-01 EVOM™ EndOhm-6 Electrode for TEER in 6 mm Insert EVM-EL-03-01-02 EVOM™... -
Page 30: Troubleshooting
Defective charger The EVOM™ Manual charge socket can be tested with a USB to Mini-B cable plugged into a computer’s USB port (WPI #803026). If the charging LED lights up, then a new charger is needed (WPI #803025). Not all USB See "Sample Resistance File (*.CSV)"... - Page 31 EVOM™ Manual Issue Possible Cause Solution Electrodes are Clean the electrodes as per the instruction manual for heavily charged the electrode. or dirty Electrode too Move the system to a different area away from sources close to strong of electromagnetic fields. Electromagnetic field sources electromagnetic could include computers, MRI equipment, magnetic radiation device...
- Page 32 Issue Possible Cause Solution Cell culture or If the cell culture has been given sufficient time to achieve media problem confluence, and the reading is stable but significantly lower than expected, then the problem is probably related to the cell culture. Electrode failure will not generally cause a lower than expected yet stable reading.
- Page 33 NOTE: The EVOM™ Manual will read only about 5% above 100K Ω. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or wpi-ts@wpiinc.com. *Drift–Readings that continuously increase or decreases a significant value (either voltage or resistance) over time.
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Page 34: Specifications
Power Consumption ......................~250 mA Certifications ............................ CE Firmware ........................Upgradeable* *NOTE: A USB to Mini-B cable (WPI #803026) is required along with PC bootloader software and the image are required to upgrade the firmware. **Not user serviceable. Contact WPI for repair or replacement. -
Page 35: Appendix A: File Formats
EVOM™ Manual APPENDIX A: FILE FORMATS Resistance (*.CSV) Date 03/12/2020 13:11:56 Well Plate 24 EVOM™ Manual Version 2.0 MODE OHMS offset 999.8 Units: OHMS Direction 1 Resistance Scale 50k Ohms 19015 19015 19015 19015 19015 19015 19016 19016 19016 19016 19016 19016 19016... -
Page 36: Running The External Data Connection Output Program (Pc, Mac, Linux)
Program (PC, Mac, Linux) Windows 8 or above users: WPI provides a USB keydrive (also known as a thumb drive) with all the software needed to run the External Data Output program. To use these programs: Connect the EVOM™ Manual to the USB port of the PC. The active remote connection port for the EVOM™... - Page 37 This archive has all the Python program files to use the external data output. Extract the files to a WPI directory or a directory of your choice. Connect the EVOM™ Manual to the USB port of the PC. The active remote connection port for the EVOM™...
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Page 38: Sample Csv Recording
10. The default file name is WPI_EVOM_Manual.csv. This file may be opened in Microsoft® Excel or any other spreadsheet program as a .CSV file Sample CSV recording TIME , DATA , VALUE 12 Mar 2020 13:24:35,0,0000.0 12 Mar 2020 13:24:36,1,19017.9 12 Mar 2020 13:24:36,2,19018.1 12 Mar 2020 13:24:36,3,19018.0 12 Mar 2020 13:24:37,4,19018.1... -
Page 39: Appendix C: Improving The Accuracy And Repeatability Of The System
For the highest accuracy in measurement, WPI recommends disconnecting the unit from the AC power source. Choose an electrode based on your application (size of the inserts: 6 mm, 12 mm, 24 mm and transwell type). -
Page 40: Appendix D: Resistance Calculations
APPENDIX D: RESISTANCE CALCULATIONS Resistance The value of the blank (control well) always adds to the total resistance measured across a tissue culture membrane. The blank resistance must be measured and then subtracted from the resistance reading across the tissue in order to obtain the true tissue resistance. -
Page 41: Teer Calculations
EVOM™ Manual = 670 Ω * 3.14* 3.25* 3.25 / 100 = 670 Ω * 0.331 cm = 221.77 Ω-cm 221.77 Ω is the resistance of a unit area of 1 cm (TEER). The larger the membrane, the lower the resistance. The dimension is Ω-cm , not Ω... -
Page 42: Declaration Of Conformity
175 Sarasota Center Boulevard Sarasota, FL 34240-9258 USA As the manufacture/distributor of the apparatus listed, declare under sole responsibility that the product(s): WPI PN: EVOM3 To which this declaration relates is/are in conformity with the following standards or other normative documents: Safety:... -
Page 43: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 44 W OR LD P RE C I SI O N I NSTR U M E N TS...
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