Related Manuals for WPI EVOM EVA-MT-03 Series
Summary of Contents for WPI EVOM EVA-MT-03 Series
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL EVA-MT-03-XX EVOM™ Auto Automated TEER Measurement System with 24 & 96 Capability (v. 3) Serial No._____________________ www.wpiinc.com 092823...
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Page 3: Table Of Contents
EVA-MT-03-XX CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Features............................2 Notes and Warnings:........................ 3 Parts List............................3 Unpacking ........................... 4 INSTRUMENT DESCRIPTION ......................5 EVOM™ Auto System........................ 5 Autosampler..........................6 Interface Unit ..........................7 Software ............................8 Online/Offline Toggle......................9 Resistance Value Display .................... - Page 4 APPENDIX A: UPDATING EVOM™ AUTO SOFTWARE............42 APPENDIX B: USING AN ETHERNET CONNECTION ..............44 INDEX...............................45 DECLARATION OF CONFORMITY ....................46 WARRANTY .............................47 Claims and Returns ........................47 Repairs............................47 World Precision Instruments...
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Page 5: About This Manual
EVA-MT-03-XX ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. -
Page 6: Introduction
INTRODUCTION The EVOM™ Auto automates TEER measurements. The instrument is controlled through a web browser, and the measurements are stored in the instrument and downloaded to your computer or iPad (control device) through the web browser. At present, the instrument supports MatTek , Corning, and Millipore 96 HTS well plates using the 96-well electrode array, Corning 24 HTS plates using the 24C electrode array, and Millipore 24 HTS plates using the 24M electrode array. -
Page 7: Notes And Warnings
EVA-MT-03-XX Notes and Warnings: CAUTION: Never set anything (especially liquids) on top of the autosampler. CAUTION: When you move or relocate the instrument, remove the front cover/lid, which can fall off. For example, you may move the system in and out of a cell culture hood. -
Page 8: Unpacking
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 39 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or wpirms@wpiinc.com. -
Page 9: Instrument Description
EVA-MT-03-XX INSTRUMENT DESCRIPTION EVOM™ Auto System Fig. 2—The EVOM™ Auto System, includes the iPad, the autosampler, the interface unit and the power supply (not shown). The EVOM™ Auto consists of these major components: • Autosampler – The robot contains the electrodes, a 3-compartment rinse stations, and a well plate area. -
Page 10: Autosampler
Autosampler Fig. 3—The autosampler takes the TEER measurements of the HTS well plate. Here are the primary components of the autosampler. Electrode Array Head – The electrode array rests in the home position (shown in Fig. 3), just to the left of the rinse stations. The LED on the electrode array will: •... -
Page 11: Interface Unit
EVA-MT-03-XX Looking at the autosampler measurement plate from the top, you can see the: • Rinse Station Pocket where the rinse station slides into place. • Plate Positioner Sockets for installing the Plate Positioner used for positioning the well plate precisely. •... -
Page 12: Software
Software The EVOM™ Auto software runs in a web browser and is not tied to any particular operating system or device. A pre-configured iPad is provided with the system. Fig. 7—The Main screen gives you access to all the main menu items and an overview of the selected plate. -
Page 13: Online/Offline Toggle
EVA-MT-03-XX Online/Offline Toggle The system can only use the autosampler and collect data when it is in the Online state The toggle switch in the grey Current State area of the window determines the online status. Use that switch to change the status of the autosampler. The system can only use the autosampler and collect data when it is in the Online state. - Page 14 Fig. 10—The emergency stop button is located in the bottom right corner of any window where the electrode array can move. Press this button to disable the autosampler motors and stop movement immediately. The only valid option after this is Go Home. The autosampler needs to find the home position again to regain the reference to all movement.
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Page 15: Setup
If a connection is unsuccessful, ensure that the iPad is connected to the correct Wi-Fi network for your EVOM™ Auto. Then, click on the WPI icon (Fig. 26). If you are not using the provided iPad tablet, open a browser and navigate to https://192.168.54.1:5000. - Page 16 Fig. 14—Ready message displays briefly. Fig. 15—Home screen showing a 96-well setup. Fig. 16—Home screen showing a 24-well setup. World Precision Instruments...
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Page 17: Changing The Electrode Array Head
EVA-MT-03-XX If your connection between the iPad and the instrument is interrupted, you will see the greyed out screen with the Instrument Disconnected notice overlaid. This can happen if the unit loses power or the network connection is disrupted. Ensure your unit has power and the Wi-Fi adapter is correctly installed. - Page 18 NOTE: The electrode array needs to be installed before trying to power up the unit, otherwise the software will not recognize the device and will not connect. Fig. 18—Depress the button on the left side of the electrode array until you hear a click. Then, pull the array out to remove the electrode array.
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Page 19: Using A Different Plate
EVA-MT-03-XX Using a Different Plate First, install the matching electrode array. For example, if you want to use a Corning 24 HTS plate, install a 24C electrode array. See “Changing the Electrode Array Head” on page 13. Insert the appropriate plate positioning adapter key. In our example, use the C24/ M24 adapter key. -
Page 20: Operating Instructions
TIP: The same sequences may be used on multiple plates. Full Screen Mode By default the system runs in full screen mode when you start it using the WPI icon. If you run the software from a browser by entering the URL directly, you will want to put the system into full screen mode, because the software is easier to operate when it runs in full screen mode. -
Page 21: Selecting A Plate Brand And Plate Profile
EVA-MT-03-XX Selecting a Plate Brand and Plate Profile The plate brand selected displays in the upper right corner of the window. Selecting a brand loads the geographical coordinates for the specific plate you choose. The electrode array is configured to support various 24 or 96 HTS well plates. Three different brands of plates are supported by the 96 electrode array: •... -
Page 22: Online Status Definition And Use
Select the desired brand (in offline mode), and then click on the profile choice. If you want to rename an existing profile, click on the pencil icon and enter the new name. Online Status Definition and Use The online status is displayed next to the electrode array type in the header under the EVOM™... - Page 23 EVA-MT-03-XX To create a measurement sequence, select the Manage Sequence menu. A list of the existing sequences displays (Fig. 24), and you can open and edit any existing sequence or select New Sequence to create a new one. Fig. 24—The Manage Sequence menu lists the existing sequences. If you select New Sequence, a popup allows you to name the sequence (Fig.
- Page 24 You may choose to display the measured resistance values or the TEER measurement. TEER is a calculated value based on the surface area of the well and the measured resistance. Select the Mode menu. Options include Resistance and TEER. Fig. 27—The mode menu lets you select your options. When you select the Mode>Resistance>Range, you can choose the resistance range for making measurements.
- Page 25 EVA-MT-03-XX NOTE: You may select previously created blank data sets, which will load previously measured values for the first column. Fig. 29—The blank menu lets you create a new blank or load a previous set of data for blank wells . Fig.
- Page 26 After the measurement is completed, a message appears (Fig. 32). Press OK to complete the sequence. You may re-sample the blanks as many times as desired, now or in the future. Fig. 32—The message indicates that the blank measurements have completed. 10.
- Page 27 EVA-MT-03-XX Fig. 34—The Rinse Time window lets you configure the rinse schedule. For example, the following rinse schedule will run as shown in Fig. 36. Fig. 35—This is an example of a rinse schedule. World Precision Instruments...
- Page 28 LAST COLUMN MEASURED TRAVEL TO COLUMN 1 LOWER ARRAY INTO COLUMN MEASURE WELLS TRAVEL TO RINSE STATION 2 TRAVEL TO RINSE STATION 2 LOWER ARRAY INTO RINSE STATION LOWER ARRAY INTO RINSE STATION WAIT 2 SECONDS WAIT 2 SECONDS LIFT ARRAY LIFT ARRAY WAIT 3 SECONDS WAIT 3 SECONDS...
- Page 29 EVA-MT-03-XX Fig. 37—To save the sequence, press Cancel and return to the previous window. Running an Experiment After you have defined a sequence, you can begin running an experiment. The Experiment window shows a representation of the plate on the left side of the window. On the right side of the window, you see these areas: •...
- Page 30 Plate Data Area Current State Area Details Area Plate Controls Area Schedule Controls System Controls Fig. 39—The Experiment window has a 24 well plate window with readings for a 24 Millipore HTS plate, shown in offline mode. NOTE: In offline mode the Run and other options are unavailable. Only previous collected data for a plate may be viewed.
- Page 31 EVA-MT-03-XX Set Cycles – Brings up a popup to set the number of scans to be performed using the current sequence. Set Period – Brings up a popup to set the length of time in between well plate scans. In the Plate Controls area, available options are shown in color. If an option is not available, the button will be grey.
- Page 32 To begin running an experiment: Select Experiment from the main menu. The Experiment window appears (Fig. 44). Fig. 44—The Experiment window appears. This example shows a 96-well plate option. Fig. 45—This example shows a 24-well plate option, in offline mode. World Precision Instruments...
- Page 33 EVA-MT-03-XX Select the Plate Name drop-down in the Details area on the right side of the screen. Choose from the list of existing plates or select New Plate to define a new plate. A popup appears so you can enter a name for the plate (Fig. 46). NOTE: The plate name refers to the CSV output file name where data is saved.
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Page 34: Downloading The Data File
Once the sequence is completed, a confirmation message appears. Press OK. To stop the sequence and abort the run, press the Stop button in the Plate Controls area. To pause the sequence, press the Pause button. Press the Resume button to continue the sequence where you left off. -
Page 35: Deleting A Data File
EVA-MT-03-XX Open the file with Microsoft Excel. The first line of the file indicates when the ® data was exported. Fig. 50—The CSV file shows the export date of the file in the first line. For each run there is a header section. The first run at the top of the file is the most recent. -
Page 36: Transferring Files Between Ipad And Computer
Fig. 53—Confirmation popup to verify the deletion of a plate file. Transferring Files Between iPad and Computer There are multiple ways to move files on your iPad to your Mac or Windows PC, and vice versa. • Wirelessly (email, messages, or AirDrop) – Refer to the iPad online User Guide topic Transfer files wirelessly between your iPad and computer with email, messages, or... -
Page 37: Scheduling Operations
EVA-MT-03-XX Scheduling Operations The data acquisition functions of the EVOM™ Auto may be repeated at a user-defined time interval when multiple scans of a plate are needed. The scheduled operations are displayed on the right side of the Experiment window in the Current State area (Fig. 54). The control buttons for scheduling are in the Schedule Controls area on the right side of the window (Fig. - Page 38 Fig. 58—(Left) If you select the pencil icon, you may use the keyboard to enter a four-digit number in the form of HHMM to set the period. Fig. 59—(Right) If you used the spinner to set the hours of the period, then the display changes to allow you to set the minutes of the period.
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Page 39: Stopping A Running Scheduled Sequence
EVA-MT-03-XX Stopping a Running Scheduled Sequence Press the Stop button in the Schedule Controls area to stop the schedule. The current scan will continue until it completes the cycle. The Stop and Pause buttons become active in the Plate Controls area. If you need to stop the scan, you must also press the Plate Controls Stop button. -
Page 40: Maintenance
MAINTENANCE The electrodes require regular maintenance to keep them performing optimally. Chloridizing – To replenish the chloride on the surface of the electrodes, the electrode tips need to be submerged in a bleach solution (3–6% sodium hypochlorite) for at least 10 minutes, followed by a rinse with distilled water. Stabilization –... -
Page 41: Cleaning
EVA-MT-03-XX Cleaning You can use 70% ethanol or isopropanol to disinfect the electrodes. You can include a rinsing option in the sequence. For example, your rinse protocol could include: • Rinse station 1– 70% Ethanol • Rinse station 2 – Sterile water, media, or buffer •... -
Page 42: Chloriding The Electrode Array
Chloriding the Electrode Array Select Maintenance on the main menu. The Maintenance window appears. Fig. 65—The Maintenance window. To chloridize, press the + or – buttons to set the timer to the desired time in minutes in the Chloridize frame. Select the radio button for the rinse station containing the chloridizing solution. -
Page 43: Stabilizing The Electrode Array
EVA-MT-03-XX Stabilizing the Electrode Array Select Maintenance on the main menu. The Maintenance window appears (Fig. 65). To stabilize, press the + or – buttons to set the timer to the desired time in minutes in the Stabilize frame. Select the radio button for the rinse station containing the buffer solution. Fill the appropriate rinse station with distilled water. -
Page 44: Accessories
Wi-Fi is not properly connected Restart the interface unit by cycling power to it. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at (941) 371-1003 or wpirms@wpiinc.com. World Precision Instruments... -
Page 45: Specifications
EVA-MT-03-XX SPECIFICATIONS This unit conforms to the following specifications: Autosampler Dimensions (W×D×H) ................16×10×8.4” Autosampler Weight ......................15.5 lbs. Interface Unit Weight......................... 1 lb. CE Certified.............................Yes Compatibility ..........96-well HTS Plates (Corning, Millipore, MatTek) ..................24-well HTS Plates (Corning, Millipore) Resistance Range..................10KΩ, 50KΩ, 100KΩ Number of Rinse Stations ......................3 Electrode Array for 96 HTS Plate ......Array of 8 pair of (1.2mm Φ) electrodes Electrode Array for 24 HTS Plate ......Array of 4 pair of (1.2 mm Φ) electrodes... -
Page 46: Appendix A: Updating Evom™ Auto Software
(The blue LED is ON). NOTE: Do not plug in the USB drive (WPI#: 97255) with the software upgrade yet. Establish the connection between the iPad. If any connectivity issue exist, verify that the Wi-Fi adapter is properly connected to the EVOM™... - Page 47 WPI. Now, power off the interface unit by pressing the power button. The blue LED turns off. Plug in the USB drive (WPI #: 97255) onto any available USB port. Keep the Wi-Fi adapter connected to the interface unit.
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Page 48: Appendix B: Using An Ethernet Connection
However, to achieve proper operation through an Ethernet connection, the EVOM™ Auto must be able to obtain an IP address from the selected network in an organization. WPI recommends that you consult with your IT professional to determine the best method to achieve proper connection. -
Page 49: Index
EVA-MT-03-XX INDEX Symbols run 26 –––– 9 instrument description 5 run scheduled 26 interface unit 5, 7 interface unit connection Autosampler 5, 6, 10 schedule 24, 26, 33 port 6 sequence name 29 set cycle 34 back 27 maintenance 36 set cycles 27 bad measurements 9 MatTek 17 set period 27, 33 baseline 20... -
Page 50: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 51: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 52 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
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