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Summary of Contents for WPI EVOM EVA-MT-03 Series
- Page 1 WORLD PRECISION INSTRUMENTS INSTALL GUIDE EVA-MT-03-XX EVOM™ Auto Automated TEER Measurement System with 24 & 96 Capability (v. 3) Serial No._____________________ www.wpiinc.com 092823...
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Page 3: Table Of Contents
EVA-MT-03 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................2 Features............................2 Notes and Warnings:........................ 3 Parts List............................3 Unpacking ........................... 4 INSTRUMENT DESCRIPTION ......................5 EVOM™ Auto System........................ 5 Autosampler..........................6 Interface Unit ..........................7 SYSTEM SETUP ..........................8 Setting up the System...................... - Page 4 World Precision Instruments...
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Page 5: About This Manual
EVA-MT-03 ABOUT THIS MANUAL The following symbols are used in this guide: This symbol indicates a CAUTION. Cautions warn against actions that can cause damage to equipment. Please read these carefully. This symbol indicates a WARNING. Warnings alert you to actions that can cause personal injury or pose a physical threat. -
Page 6: Introduction
INTRODUCTION The EVOM™ Auto automates TEER measurements. The instrument is controlled through a web browser, and the measurements are stored in the instrument and downloaded to your computer or iPad (control device) through the web browser. At present, the instrument supports MatTek , Corning, and Millipore 96 HTS well plates using the 96-well electrode array, Corning 24 HTS plates using the 24C electrode array, and Millipore 24 HTS plates using the 24M electrode array. -
Page 7: Notes And Warnings
EVA-MT-03 Notes and Warnings: CAUTION: Never set anything (especially liquids) on top of the autosampler. CAUTION: When you move or relocate the instrument, remove the front cover/lid, which can fall off. For example, you may move the system in and out of a cell culture hood. -
Page 8: Unpacking
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 39 of this manual. Please contact WPI Customer Service if any parts are missing at (941) 371-1003 or wpirms@wpiinc.com. -
Page 9: Instrument Description
EVA-MT-03 INSTRUMENT DESCRIPTION EVOM™ Auto System Fig. 2—The EVOM™ Auto System, includes the iPad, the autosampler, the interface unit and the power supply (not shown). The EVOM™ Auto consists of these major components: • Autosampler – The robot contains the electrodes, a 3-compartment rinse stations, and a well plate area. -
Page 10: Autosampler
Autosampler Fig. 3—The autosampler takes the TEER measurements of the HTS well plate. Here are the primary components of the autosampler. Electrode Array Head – The electrode array rests in the home position (shown in Fig. 3), just to the left of the rinse stations. The LED on the electrode array will: •... -
Page 11: Interface Unit
EVA-MT-03 Looking at the autosampler measurement plate from the top, you can see the: • Rinse Station Pocket where the rinse station slides into place. • Plate Positioner Sockets for installing the Plate Positioner used for positioning the well plate precisely. •... -
Page 12: System Setup
SYSTEM SETUP The autosampler is packed with the lid taped in place. Remove all the components from the packing materials and remove the protective tape. CAUTION: When you move or relocate the instrument, remove the front cover/lid, which can fall off. For example, you may move the system in and out of a cell culture hood. - Page 13 EVA-MT-03 Connect the power supply wall adapter to the wall outlet and insert the barrel connector into the interface unit power supply port on the back side of the interface unit. Fig. 9—Connect the power cable to the interface unit. Install the provided Wi-Fi adapter into any one of the four USB ports on the back of the interface unit.
- Page 14 The autosampler ships with a red electrode array lock in place (Fig. 11). To remove it: • Pinch the lock at the top (Fig. 12) and lift the slide on the side (Fig. 13). • Push the locking mechanism towards the right till the clasp releases from the slot in the base plate.
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Page 15: Installing The Electrode Array Head
EVA-MT-03 Make sure that there are no well plates installed on the autosampler and make sure that the rinse station block is not installed on the autosampler plate. Electrode Array Connection Port Rinse Station Well Plate Area Pocket Fig. 16—This autosampler has no well plate and no rinse station installed. When the lock is removed, you have access to the electrode array connection port. - Page 16 Remove the red electrode protection shield by releasing the Velcro strap. Fig. 18—Remove the protective sleeve. Make sure that the autosampler is in the home position on the left side of the unit. Locate the electrode array connection port on the back wall of the autosampler. Fig.
- Page 17 EVA-MT-03 CAUTION: To safely change the electrode array, the software must be in offline mode OR the unit must be powered off. If one of these conditions is not met, DO NOT try to change the electrode array. Removing the electrode array when the system is in the fully active mode may put the system in an unresponsive, disrupted communication mode that may necessitate a return to the factory.
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Page 18: Starting The System
An iCloud account should be established to upload files. When you receive it, the iPad is configured to begin using the autosampler, but WPI recommends that you register it to your organization for optimal use of the device. - Page 19 If a connection is unsuccessful, ensure that the iPad is connected to the correct Wi-Fi network for your EVOM™ Auto. Then, click on the WPI icon (Fig. 26). If you are not using the provided iPad tablet, open a browser and navigate to https://192.168.54.1:5000.
- Page 20 Fig. 27—Main window. This is your main window. Keep the online slider enabled to perform experiments. • The Experiment menu to select a sequence and start an experiment. • The Manage Sequence menu to create or modify a sequence. The Expert menu lets you adjust the plate electrode coordinates (positioning). •...
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Page 21: Lost Connection
EVA-MT-03 Lost Connection If your connection between the iPad and the instrument is interrupted, you will see the greyed out screen with the Instrument Disconnected notice overlaid. This can happen if the unit loses power or the network connection is disrupted. Ensure your unit has power and the Wi-Fi adapter is correctly installed. -
Page 22: Installing The Rinse Stations
Installing the Rinse Stations Ensure that the electrode array is in the home position. Navigate to the Experiment window on the tablet and press the Go Home button. Slide the rinse station into the pocket on the left side of the measurement plate. Fig. -
Page 23: Installing The Well Plates
EVA-MT-03 Installing the Well Plates Ensure that the electrode array is in the home position. Three plate positioners are included with the system. Choose the one that corresponds with your plate. Locate the three plate positioner slots in the base plate and line up the plate positioner with the slots. -
Page 24: Selecting A Plate Brand And Plate Profile
Fig. 35—Use the plate positioner to align the well plate. Make sure the plate is secured in between plate positioner and well plate handle and sitting flat onto the base surface. Selecting a Plate Brand and Plate Profile The plate brand selected displays in the upper right corner of the window. Selecting a brand loads the geographical coordinates for the specific plate you choose. -
Page 25: Verifying Alignment
EVA-MT-03 NOTE: On the Expert window, you may also select the brand from the displayed drop-down list. Fig. 36—Click on the brand in the upper right corner under the EVOM™ Auto logo to access the plate brand and plate profile menu. Fig. - Page 26 Select the Expert menu to open the Expert window. Press the Measure Position button. The electrode array travels horizontally and then lowers the electrodes to the measurement depth. Once the electrode is positioned just above the height of the membrane, use the height adjustment fixture to verify the depth or adjust it using the up or down button in the software.
- Page 27 EVA-MT-03 Fig. 42—A 24-well plate option in the offline mode is shown here. Remove the depth adjustment fixture and install the matching plate positioner and the matching well plate. For example, the C24/M24 plate positioner and the Corning 24 well plate are used when a 24C electrode is plugged in. Fig.
- Page 28 From the Plate Name drop-down field in the Details area on the right side of the window, select Alignment Check. From the Sequence Name drop-down field, select Alignment Sequence. This sequence directs the electrode array to travel to the first column and lower the electrode array to the measurement height.
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Page 29: Appendix A: Using Expert Mode To Position Well Plate
EVA-MT-03 APPENDIX A: USING EXPERT MODE TO POSITION WELL PLATE In Expert mode, you can adjust the positions of the first column in the X direction and the depth of measurement (Z direction). If the position of the electrodes needs to be adjusted, select the Expert Mode menu. -
Page 30: Saving A Profile Setting
Fig. 46—The Expert window has two sections: well plate adjustments and rinse station adjustments. Saving a Profile Setting To save a profile, click on the Save button at the bottom of the window. A dialog box opens asking you to verify whether you want to save the profiles settings for that plate profile. -
Page 31: Well Plate Adjustments
EVA-MT-03 If an electrode array encounters a crash with the plate, no matter what type of error occurred (wrong plate or wrong plate positioner or plate-electrode coordinates being off), an emergency stop is automatically invoked. As soon as the the electrode array touches a solid surface (crash), an the emergency stop mechanism is initiated to prevent any damage to the electrode array. -
Page 32: Adjusting The Electrode Array Position
Up and Down Buttons – These buttons move up or down in the Z direction. Use this adjustment to set the measuring height to make sure it does not puncture the membrane on the well plate. When using the depth adjustment fixture make sure that none of the electrodes touch the plate surface and some clearing distance is added. - Page 33 EVA-MT-03 Press the Travel Position button in the Well Plate Adjustments area. This places the electrode array above the depth adjustment fixture where the first column of wells should be. Fig. 52—In the Well Plate Adjustment Area, press the Travel Position button. Press the Measure Position button to bring the electrode array to the measurement depth.
- Page 34 Fig. 54—In the Well Plate Adjustment Area, press the Travel Position button. After pressing the TRAVEL POSITION button only the left and right arrows are operational. The up and down arrows are greyed out 11. Visually verify that the electrodes are directly above the well plate openings. If necessary, use the Left and Right adjustment buttons to place the electrode array in the target area (X-axis adjustment).
- Page 35 EVA-MT-03 Fig. 56—The electrodes are properly placed in the well plate. 13. Press the Go Home button. 14. When you are satisfied with the position, click the Save button. This saves the settings, and they become the new operational set of parameters. Or, press the Back button to cancel the action.
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Page 36: Appendix B: Using An Ethernet Connection
Using a LAN cable, you may connect your interface unit directly to your LAN network. However, to achieve proper operation through an Ethernet connection, the EVOM™ Auto must be able to obtain an IP address from the selected network. WPI recommends that you consult with your IT professional to determine the best method to achieve proper connection. - Page 37 EVA-MT-03 APPENDIX D: IPAD SETUP AUTO-LOCK Ensure these iPad settings are properly configured for the best experience with EVOM™ Auto software. Check your EVOM™ Auto iPad to ensure that steps 1–4 (below) have been applied. On the iPad, navigate to Settings. Then, select Display & Brightness, and go to Auto- Lock at the bottom.
- Page 38 From the home screen, while holding the iPad in landscape position, press the upper right corner of the screen and drag to the center without lifting the finger. This will bring up the following shortcut screen. Fig. 60—Press the landscape auto lock. The icon will turn red. World Precision Instruments...
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Page 39: Appendix D: Adding Link To Ipad Home Screen
EVA-MT-03 APPENDIX D: ADDING LINK TO IPAD HOME SCREEN By adding an EVOM™ Auto icon to the home screen of your iPad, you can open the program in full screen mode. If you do not have a link on your iPad to connect to the EVOM™... - Page 40 Enter “EVOM Auto” (or your preferred name for the icon) in the Add to Home window and click Add in the upper right corner. The new icon appears on your home screen. Tap the icon to open the web page in full screen mode. World Precision Instruments...
- Page 41 EVA-MT-03 World Precision Instruments...
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Page 42: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 43: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 44 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
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