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Summary of Contents for WPI ISO2
- Page 1 WORLD PRECISION INSTRUMENTS Instrumenting scientific ideas INSTRUCTION MANUAL Isolated dissolved oxygen meter and oxygen electrode Serial No._____________________ www.wpiinc.com 030416...
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Page 3: Table Of Contents
ISO2 CONTENTS ABOUT THIS MANUAL ........................1 INTRODUCTION ..........................1 Parts List ............................2 Unpacking ........................... 2 INSTRUMENT DESCRIPTION ......................3 Sensor Structure and Assembly .................... 3 Recorder Output ........................3 OPERATING INSTRUCTIONS ......................4 Calibration........................... 4 Polarization ........................... 4 Zero (Oxygen) Point Calibration.................. - Page 4 World Precision Instruments...
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Page 5: About This Manual
IMPORTANT NOTE: The OXELP oxygen sensor is specifically designed to be used with WPI’s ISO dissolved oxygen meter. To use the OXELP with other instruments, see Fig. 2 on page 3 for plug information. -
Page 6: Parts List
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 19 of this manual. Please contact WPI Customer Service if any parts are missing at 941-301-1003 or customerservice@wpiinc.com. -
Page 7: Instrument Description
Membrane–Gas permeable, polymeric membrane covering the end of the stainless sleeve to separate it from the external environment Sleeve–Disposable, protective stainless steel sleeve (WPI#5378) that houses the sensitive electrode pair. The sleeve is flanged to properly connect with the locking cap. -
Page 8: Operating Instructions
OPERATING INSTRUCTIONS Calibration For accurate results the sensor should be calibrated as closely as possible to the temperature and medium at which the measurement is to be made (if measurements are in gas, calibrate in gas). To calibrate the sensor: Turn on the ISO , and polarize the sensor (“Polarization”... -
Page 9: Zero (Oxygen) Point Calibration
ISO2 Zero (Oxygen) Point Calibration The true electronic zero reading can be obtained by removing the sensor from the meter. At that point, the meter has no current running through it and is at the electronic zero. The meter should read zero. As soon as you connect the sensor to the meter, you can have a small current leakage, called the baseline current. -
Page 10: Scale Factor Adjustment
CAUTION: Wear nitrile gloves when handling sodium sulfite (Na Carefully slide the tip of the OXELP sensor through the hole in the top of the bottle cap. Position the sensor so that the probe handle rests against the bottle cap. If the bottle is top heavy, you may need to support the sensor. The current should drop rapidly (after a few seconds) to a value of 0nA. - Page 11 ISO2 If you use pure oxygen, the display should read 100. If the display does not show the correct value, use a small screwdriver to adjust the % adjustment screw so that the meter reads 100%. TIP: Air can also be used as a calibration standard, but since water vapor affects the sensor reading it is best to use dry air unless the ambient humidity is accurately known.
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Page 12: Calibration For O In Living Tissue/Blood
the solution temperature is 25°C, for example, the proper oxygen reading when the sensor is in fresh water should be 8.4 ppm. If the display does not show this value, adjust the ppm screw with a screwdriver so that the meter displays the correct value. -
Page 13: Calibration For O 2 Maintenance
The use of the sensor in other fluids or pressures may damage the membrane. Organic solvents must not be used. If you have a particular solution that may be in question, please contact WPI for information. The sensor is relatively durable, except for the membrane sleeve. Exercise caution when handling any sensor to avoid actions that could damage the sensor tip. -
Page 14: Cleaning The Membrane
If the probe was used in a protein-rich solution, the tip should first be soaked in a protease solution for several minutes to remove protein build-up, and then in distilled water. Enzymatic detergent (for example, Enzol, WPI#7363) can also be used. Accumulated organic matter can be removed by briefly immersing the tip in a 0.1M HCl or 0.1M NaOH (at times both may be necessary) for 10 seconds. - Page 15 (for example, 60–300nA). Then, the current value will beging to fall. Using the MicroFil™ nonmetallic syringe needle (WPI #MF28G67-5) and 1mL plastic syringe (included in the start-up kit) inject approximately 100µL of electrolyte directly into the new sleeve. Insert the MicroFil in as far as it will go, and slowly draw the MicroFil out of the sleeve as it fills.
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Page 16: Cleaning The Electrode Surface
Cleaning the Electrode Surface The reduction of oxygen causes a decrease in the surface activity of the working electrode which gradually “poisons” it. This poisoning reduces the redox current from the reduction of oxygen. In order for the ISO to function properly, the minimum current required at room temperature and 21% oxygen (air) is 15nA. -
Page 17: Troubleshooting
ISO2 TROUBLESHOOTING Issue Possible Cause Solution Sensor is shorted Test the sensor with the dry sensor test. See “Dry Sensor Test” on page <OV>. Sensor surfaces may be coated Try the clean function and retest. See “Cleaning the Electrode Surface” on page... - Page 18 NOTE: If the sensor does not read a high nA value on refilling the membrane sleeve, then an air pocket may be trapped in the bottom of the sleeve. Use care in the refilling process with the correct length of Microfil (WPI #MF28G67-5) and gently tap the sensor to dislodge these air pockets.
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Page 19: Appendix
ISO2 SPECIFICATIONS This unit conforms to the following specifications: Modes ..............% O : 0–100%, ppm: 0–20, Current: 0–199.9nA Resolution ..........................0.1 ppm Accuracy ............................. ±1.5% Output Resistance ........................1000Ω Display ..........................3.5-digit LCD Recorder Output ........1mv = 1nA (i.e., 10mV = 10% O = 10ppm = 10nA) Power ........ - Page 20 Table 1b: Solubility of Oxygen in Seawater °C 5 g/L 10 g/L 15 g/L 20 g/L 13.8 13.0 12.1 11.3 13.4 12.6 11.8 11.0 13.1 12.3 11.5 10.8 12.7 12.0 11.2 10.5 12.4 11.7 11.0 10.3 12.1 11.4 10.7 10.0 11.8 11.1 10.5...
- Page 21 ISO2 Table 3: Water-vapor Partial Pressure Temp. Temp. °C mmHg °C mmHg Table 4: Bunsen Coefficients (a) for Solubility of Oxygen in Plasma and Blood Blood Hb g/100 mL Temp °C Plasma 10 g 15 g 20 g 0.0302 0.0310 0.0312...
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Page 22: Declaration Of Conformity
DECLARATION OF CONFORMITY World Precision Instruments... -
Page 23: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 24 International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Zossener Str. 55, 10961 Berlin Tel: 030-6188845 •...
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