Determination Of G-Values - Labnet Prism C2500-100V Instruction Manual

10.0 Determination of G-values

The centrifuging radius of the 1.5/2.0 mL rotor is 8.23 cm. The Labnet Prism™ microcentrifuge has an automatic g-force conversion
program, so g-values are automatically calculated and can be displayed on the control panel, for this centrifuging radius. If adapters
or smaller tubes are used, the centrifuging radius changes as does the g-force. The chart below can be used to manually determine
g-values for any centrifuging radius.
25,000
20,000
15,000
10,000
6000
4000
3000
2000
1000
500
200
To calculate the RCF value at any point along the tube or bottle, measure the radius (in mm), from the center of the centrifuge spindle
to the particular point. Draw a line from the radius value on the right-hand column to the appropriate centrifuge speed on the left-
hand column. The RCF value is the point where the line crosses the center column. The nomogram is based on the formula:
RCF = (11/17 x 10 ) RN
-7 2
where:
R = Radius in mm from centrifuge spindle to point in tube bottom
N = Speed of spindle in RPM
Speed of Centrifuge
Spindle in RPM
30,000 Relative Centrifugal
Force (x g)
20,000
10,000
5000
2000
1000
500
200
100
50
20
10
0
500
Radius in mm from Center of
475
Centrifuge Spindle to Point
450
Along Tube or Bottle
425
400
375
350
325
300
275
250
225
200
175
150
140
130
120
110
100
90
80
70
60
50
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