Rotor Maintenance; Removing And Installing The Angle Rotor; Loading The Rotor; Overloading Rotors - Labnet Prism C2500-100V Instruction Manual

6.2 Rotor Maintenance

The rotor should be cleaned thoroughly after each use. NOTE: Thorough cleaning must be performed when spinning samples
containing phenol or phenol chloroform. Periodically inspect the rotor for dents, dings, scratches, discoloration, and cracks. If any
damage to the rotor is found, discontinue use of the rotor immediately and replace.

6.3 Removing and Installing the Angle Rotor

The Labnet Prism™ microcentrifuge comes complete with a standard 24-place rotor installed. To remove the rotor for cleaning,
using the rotor wrench remove the rotor securing screw from the motor shaft by turning the screw counterclockwise. Lift the rotor
directly upward in a straight vertical motion.
To replace the rotor, first make sure the motor shaft and rotor mounting hole are clean. Place the rotor on the motor shaft.
Reinstall the rotor securing screw on the motor shaft by turning it clockwise. Hold the rotor with one hand and tighten the rotor
securing screw, using the rotor wrench.

6.4 Loading the Rotor

Tubes to be loaded should be filled equally by eye. The difference in the weight between the tubes should not exceed 0.1 gram. Tubes
should always be loaded so there is equal spacing between all tubes. One or two additional loaded tubes may need to be added to
achieve this. See Figure 1 for a typical balancing scheme.

6.5 Overloading Rotors

The maximum load of the rotor and maximum speed has been established by the manufacturer. Do not attempt to exceed these
values. The maximum speed of the rotor has been established for liquids having a homogeneous density of 1.2 g/mL or less. In order
to centrifuge liquids with a higher density it is necessary to reduce the speed. NOTE: Failure to reduce the speed may result in damage
to the rotor and centrifuge. The revised maximum speed can be calculated with the following formula:
Example: Where the density of the liquid is 1.7, the new maximum speed would be calculated as follows:
If in doubt concerning maximum speeds, please contact the manufacturer for assistance.
4 | LABNET PRISM MICROCENTRIFUGE
Figure 1. Loading the rotor to achieve balance.
Reduced speed (n ) =
higher density value
red
Reduced speed (n
1.2
(n ) = × 15,000 = 12,602 rpm
1.7
red
1.2
(n ) = × 15,000 = 12,602 rpm
1.7
red
1.2
× max. speed (n
1.2
) = × max. speed (n
higher density value
red
)
max
)
max