Use Of Oil Immersion; Lamp Replacement - Bresser SCIENCE TRM-301 Instruction Manual

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Fig. 9: Using the 100x oil immersion objective.
WARNING: If the immersion oil comes
into contact with skin, rinse it off
thoroughly with soap and water. In the
event of eye contact, rinse the eyes under
running water for at least 15 minutes.
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Fig. 10: Replace the lamp.
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7. USE OF OIL IMMERSION

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a) Focus the object with the 40x objective (1).
b) Move the longitudinal XY stage (2) to expose the condenser lens (3). Turn
the 40x lens (1) to the side.
c) Apply a drop of immersion oil (4) to the lens of the condenser (3).
d) Move the slide (5) back into the beam path using the longitudinal XY stage
(2).
e) Put another drop of immersion oil (4) on the cover glass of the slide (5)
and screw in the 100x objective (6), immersing the front lens in the oil.
f) Slowly refocus the image using the fine adjustment knob (7).
NOTE: Oil immersion can only be performed with a suitable 100x objective.
The 100x oil immersion objective is included with the product.
NOTE: We recommend immersion oil with a refractive index (RI) of
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nD=1.515 in accordance with ISO 8036.
NOTE: As air bubbles in the oil impair the image quality, make sure
that the oil drop is free of bubbles. To remove bubbles, defocus the oil
immersion objective repeatedly and refocus it, or switch to another
objective and allow the visible bubbles to burst before immersing the 100x
objective again.
NOTE: Clean the 100x lens by dabbing off the oil with a soft baby paper
towel (lotion-free). If you use the immersion lens regularly, the lens should
only be cleaned with a damp cloth once a week. Use a solution of 70%
ethanol or isopropanol (to prepare such a solution, use p.A or pharmacy
alcohol and double-distilled water).

8. LAMP REPLACEMENT

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a) Switch off the on/off switch (main switch) (1), disconnect the plug from the
mains socket, and then disconnect the power adapter (2) from the mains
connection (3) on the microscope. Remove the blue filter from the field
diaphragm.
b) Carefully tilt the microscope and loosen the fastening screw (4) on the
bottom flap (5), which carries the lamp holder and is located in the middle
of the underside of the microscope. Open the bottom flap (5).
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c) Pull the old halogen bulb (6) (12V/ 30W) out of the lamp holder (7).
WARNING: Exercise caution, as the lamp may still be hot!
d) Plug a suitable replacement halogen bulb (6) (12V/ 30W) into the lamp
holder (7).
NOTE: Take care not to touch the lamp directly with your fingers (to
prevent soiling of the lamp, which reduces its lifespan and intensity).
For example, grip the lamp using the lamp's protective cover or a
handkerchief.
e) Close the bottom flap (5) on the underside again with the fastening screw
➎ (4).
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f) After you have fitted the replacement lamp, reconnect the power supply and
press the on/off switch (main switch) (1). Then swivel an objective into the
beam path, adjust the condenser to the correct height using the condenser
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height drive (8), and illuminate the field of view. If the light spot shifts away
from the centre of the field of view, loosen the screw (9) slightly and move
the lamp holder (7). Guide the light spot into the centre, then immediately
tighten the screw (9) again.