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Summary of Contents for ThermoFisher Scientific Sorvall Legend Micro 17
- Page 1 Thermo Scientific Sorvall Legend Micro 17 / 17R 21 / 21R Centrifuge Instruction Manual 20058321-c • 03 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
- Page 2 How to use this manual Use this manual to get acquainted with your centrifuge and its accessories. Overleaf you will find a graphic illustra- tion of the control panel with a survey of The manual helps you to avoid inappropriate handling. the most important functions Make sure to keep it always close to the centrifuge.
- Page 3 (only for instruments with refrigeration unit) temperature speed/RCF run time quick run rotor turns open lid stop start rpm/RCF “set“ keys “set“ keys pre-temperature-regulation switch key (only for instruments with refrigeration unit) display Before switching on the centrifuge Note that “min ”...
- Page 4 Control panel Keys Display panels start: start of the centrifuge Speed / RCF stop: manually stop of a run Resting state: current value (0) or pre-selected set-point value open lid: open lid During run: present current value of speed or RCF (possible only with the instrument switched on and (after activation of switch key) standstill of the rotor)
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Page 5: Table Of Contents
Contents Contents For your safety ..........3 Handling the rotor ........21 Safety instructions in this manual......3 Rotor lid with a spring lock ......... 21 Proper use ..............4 Operation without rotor lid ........22 Improper use ............4 Rotor lid with screw plug........ - Page 6 Contents Pretemp .............. 36 Technical data ........... 57 Starting the centrifuge ..........37 Component parts ............ 57 Changing the settings during the run ..... 37 The "Easycontrol" user interface ......58 Stopping the centrifuge .......... 38 Performance ............59 Preset run time ........... 38 Electrical connections ..........
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Page 7: For Your Safety
For your safety ® Sorvall centrifuges are manufactured according to For your safety current technical standards and regulations. Nonethe- less, centrifuges may pose danger to individuals and Safety instructions in this manual surrounding if they are not used as designed This symbol denotes potential hazards they are operated by untrained personnel to persons. -
Page 8: Proper Use
For your safety Proper use being centrifuged, aerosol-tight bio-seals have to be used. The centrifuge is designed to separate liquid- For materials in a higher risk group, more than one suspended materials having different densities and precaution is required. particle size (maximum sample density is 1.2 g/cm³ at Should toxins or pathogenic substances enter the maximum speed). -
Page 9: Conformity To Current Standards
For your safety Never open the lid manually if the rotor is still turn- Conformity to current standards ing. ® Sorvall centrifuges are manufactured and tested ac- You may use the emergency lid release only in cording to the following standards and regulations case of emergency, e.g. - Page 10 For your safety for your notes...
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Page 11: Description Of The Instrument
Description of the instrument Description of the instrument Pieces delivered rotor The descriptions apply generally for all instrument variants. a special wrench for securing the rotor cable for mains connection this Manual Safety systems Micro Micro The L 17/21 and L 17R/21R EGEND EGEND... -
Page 12: Properties
Description of the instrument If you press one of the keys repeatedly, you Properties increase or decrease the corresponding pre-selected set point value stepwise. If you press the chosen key Micro Micro The L 17/21 and L 17R/21R EGEND EGEND and hold it pressed, the respective chosen measure- are tabletop centrifuges designed for the use in bio- ment value increases or decreases continuously, at... -
Page 13: Before Use
Before use Before use Transport and installation the centrifuge Where to install the centrifuge The centrifuge may only be used indoors. Its location Transport the centrifuge only in must meet the following criteria: the upright position, using the A safety zone of 30 cm around the centrifuge must special box provided with the in- be maintained. -
Page 14: Mains Connection
Before use Removing the transport protection Mains connection Make sure that the mains supply and the frequency Before using the centrifuge, make sure meet the specifications printed on the centrifuge type that the rotor transport protection has plate. been removed! First turn the mains switch of the centrifuge off (press Turn the instrument on at the power switch. -
Page 15: Accessories
Accessories Accessories You may choose from among a large variety of rotors available as accessories. In addition, there are sets of adapters and reduction sleeves for diverse commercially available vessels. Please consult our sales documentation for a complete collection of accessories including technical data and order numbers. -
Page 16: Rotors For The Legend Micro 17
Accessories Micro 17 Rotors for the L EGEND Micro 17 Table 1: Performance characteristics for L EGEND Dual Row 24 x 1.5 / 2.0 mL 36 x 0.5 mL rotor designation Rotor 18 x 2 / 0.5 mL Rotor Rotor order no. - Page 17 Accessories Micro 17 Table 1: Performance characteristics for L EGEND Hematocrit Rotor rotor designation 4 x 8 Rotor 8 x 8 Rotor 75003473 order no. 75003440 75003489 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0.5)
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Page 18: Rotors For The Legend Micro 21
Accessories Micro 21 Rotors for the L EGEND Micro 21 Table 2: Performance characteristics for L EGEND 24 x 1.5 / 2.0 mL Dual Row 36 x 0.5 mL rotor designation Rotor Rotor 18 x 2 / 0.5 mL Rotor order no. - Page 19 Accessories Micro 21 Tabelle 2: Performance characteristics for L EGEND Hematocrit Rotor rotor designation 4 x 8 Rotor 8 x 8 Rotor order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0.5)
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Page 20: Rotors For The Legend Micro 17R
Accessories Micro Rotors for the L EGEND Micro 17R Table 3: Performance characteristics for L EGEND 24 x 1.5 / 2.0 mL Dual Row 36 x 0.5 mL rotor designation Rotor Rotor 18 x 2 / 0.5 mL Rotor order no. 75003424 75003436 75003418... - Page 21 Accessories Micro 17R Table 3: Performance characteristics for L EGEND Hematocrit Rotor rotor designation 4 x 8 Rotor 8 x 8 Rotor order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0.5)
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Page 22: Egend 21R
Accessories Micro 21R Rotors for the L EGEND Micro 21R Table 4: Performance characteristics for L EGEND 24 x 1.5 / 2.0 mL Dual Row rotor designation 36 x 0.5 mL Rotor Rotor 18 x 2 / 0.5 mL Rotor order no. - Page 23 Accessories Micro 21R Table 4: Performance characteristics for L EGEND Hematocrit Rotor rotor designation 4 x 8 Rotor 8 x 8 Rotor order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0.5)
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Page 24: Adapter
Accessories Adapter Table 5: Adapter max. tube tube capacity number color no. of Adapter for dimensions [ ml ] per set 24 x 1.5 / 2.0 mL Rotor 75003424 order x length Dual Row 18 x 2.0 / 0.5 mL Rotor 75003418 [ mm ] reduction sleeve PCR 6.2 x 20... -
Page 25: Handling The Rotor
Handling the rotor Rotor lid with a spring lock Handling the rotor Opening Rotor temperature The rotor lid is held on the central rotor nut integrated The rotors are only to be used within into the rotor the temperature range from - 9 °C to +40 °C. -
Page 26: Operation Without Rotor Lid
Handling the rotor - Closing Operation without rotor lid For closing the rotor place the rotor lid centrically on If you are going to operate the rotor without the rotor lid the rotor nut. the aerosol-seals must be removed prior to use. Press the rotor lid down now until the lock is audio- visually locked Aerosol-seals... -
Page 27: Rotor Lid With Screw Plug
Handling the rotor Rotor lid with screw plug For this kind of rotor the rotor lid will be held centrically These rotors are not designed for on the rotor collar. aerosoltight applications! The O-Ring in the rotor collar pro- vides only for tight fit of screw plug. The external lid lip can not be sealed for these rotors. -
Page 28: Aerosol-Tight Application
Handling the rotor Tubes are only to be filled to a level where the sample Aerosol-tight application does not reach the brim of the tubes during centrifuga- Only with the designated rotors! tion. See the "Rotor tables" from page 12. Please observe the permissible filling volumes! Nominal volume: Permissible volume:... - Page 29 Handling the rotor Correct operation when filling the sample tubes and The following steps have to be carried out: closing the rotor lid are prerequisites for aerosol bio- Lubricate the seals before inserting them containment. (lubricant order no. 76003500) Prior to each application, the seals in the rotors have to be checked for correct fit, abrasion or damage and have to be slightly greased.
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Page 30: Checking For Aerosol-Tightness
Handling the rotor Checking for aerosol-tightness For a quick test one can check the fixed angle rotors The checking of the rotor type and bucket was done according to the following procedure: according to the dynamic Microbiological test proce- Slightly grease all seals. dure with regard to EN 61010-2-020 appendix AA. -
Page 31: Operation
Operation Operation Opening the lid Switching on the centrifuge For activating the lid release the centrifuge Set the main power switch of the instrument. must be connected to mains and switched For a couple of seconds the following reading appears in the control panel: (the temperature display field only for cooled instrument) To open the lid press the „open-lid“-button... -
Page 32: Installing The Rotor
Operation Proceed as follows: Installing the rotor 1. Open the centrifuge lid and make sure that the Improper or improperly combined rotor chamber and the rotor are clean. Remove accessories may cause severe dam- eventual dust, foreign material or sample residues. age to the centrifuge! The thread and the O-Ring on the motor shaft must be in perfect condition. - Page 33 Operation 4. Press the rotor gently down until it stops. 5. Grip the rotor tightly and use the provided rotor wrench to tighten up the rotor. Do not push the rotor down using force. If the rotor can not be tightened, you have to remove it carefully, align the notch and the retaining pin again and re-install it.
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Page 34: Loading The Rotor
Operation Filling the centrifuge tubes Loading the rotor Maximum load Please note that plastic sample vessels only have a limited service life - particularly when Overloading may cause the rotor used at maximum performance (rpm or tem- to explode! perature) - and must be replaced as neces- sary! Exploding parts may severely damage the centrifuge! -
Page 35: Placing The Tubes In The Rotor
Operation Placing the tubes in the rotor Uneven loading may lead to damage of The rotor must be loaded symmetrically. When loading the rotor and centrifuge in extreme the rotor only partially, you must ensure that opposite cases. Imbalance not only causes a bores always receive tubes of equal weight (when cen- noisy run, but also causes premature trifuging a single sample, place a centrifuge tube e.g. -
Page 36: Entering Parameters
Operation Entering parameters Switching from speed to RCF display By pressing the key briefly, you increase or decrease Upon turning on the centrifuge, the speed display is the speed in one step (of set. 100 rpm). By activating the selection key you can switch to the This option is supposed to RCF- value or change between rpm and RCF displays. -
Page 37: Entering The Rcf Value
Operation Entering the RCF value Concerning the RCF value The relative centrifugal force (RCF) is given in multi- You can adjust the RCF pre-selected value in steps of ples of the earth gravity g. It is a dimensionless number 100 g. The setting of pre-selected value is entered value that allows one to compare the efficiency of sep- analogously to the speed. -
Page 38: Selecting The Run Time
Operation Selecting the run time 3. Release the key as soon as you have reached the You can select a run time between 1 and 99 min or desired value and fine tune if necessary by repeat- continuous operation [hd]. edly briefly pressing one of the keys. -
Page 39: Setting The Temperature
Operation Setting the temperature 3. Release the key as soon as you are close to the To determine the sample temperature for instruments desired value and fine tune if necessary by repeat- with refrigeration unit, operate as adviced: edly briefly pressing the key. The display flashes for 1. -
Page 40: Pretemp
Operation Pretemp If you wish to change the temperature of your samples, The pretemp-function allows quick and easy tempering please consider that the time required for temperature of the unloaded rotor. adjustment is prolonged. The farther apart initial and After calling the function by pressing the key only final temperature, the longer it takes for the tempera- the desired temperature still must be chosen. -
Page 41: Starting The Centrifuge
Operation Changing the settings during the run Starting the centrifuge You can change the settings while the rotor is spinning. Once the rotor is properly installed, the main switch Through a one-time pressing of any key of the turned on and the lid closed, you can start the centri- control panel, the current value will switch into fuge. -
Page 42: Stopping The Centrifuge
Operation Stopping the centrifuge Short-time centrifugation Preset run time For short-term operation, the centrifuge is equipped with the "quick run" function. Normally the run time has been pre-selected, and all you have to do, is to wait until the centrifuge terminates Short-term centrifugation is started by pressing the the run automatically. -
Page 43: Removing The Rotor
Operation Removing the rotor Audible alarm To remove the rotor you have to proceed in the reserve Accompanying all error messages, a warning signal is order as at the installation of the rotor. given out which can be silenced upon pressing any key. -
Page 44: Turning Off The Centrifuge
Operation Turning off the centrifuge WEEE Compliance: The centrifuge is turned off by switching the main This product is required to comply with the European switch into the "0" position. Union`s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the following symbol: The main power switch should be turned off after a complete centrifugation run. -
Page 45: Maintenance And Care
Maintenance and care Cleaning Maintenance and care Maintenance to be performed by the Pull mains plug before cleaning the customer instrument! For the protection of persons, environment and materi- al you are obliged to clean the centrifuge regularly and Clean the casing, the rotor chamber, the rotor and the to disinfect it if necessary. -
Page 46: Cleaning The Filter Unit
Maintenance and care Please clean the venting slots regularly! To protect the refrigeration unit, the cooled devices are equipped with an air filter unit. Depending environment situation Sucking grid recommended to clean the filter at least every three months. Disconnect the centrifuge from mains switch before cleaning the venting slots and the filter unit. -
Page 47: Disinfection
Maintenance and care Disinfection If a centrifuge tube containing infectious material Rotor and rotor chamber must be treated with a neu- leaks, during a centrifugation run, you have to disinfect tral, universal desinfectant. Best suited for this purpose immediately the rotor, and/or also the centrifuge. are disinfectant sprays, ensuring that all rotor and ac- cessory surfaces are covered evenly. - Page 48 Maintenance and care 5. Treat the rotor and the rotor lid according to the Disinfection with bleaching lye instructions given for the disinfectant (soaking in liquid or spraying). You must strictly observe the These agents contain highly ag- specified action times! gressive hypochlorites and must 6.
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Page 49: Decontamination
Maintenance and care Decontamination The rotor must be cleaned and rinsed with distilled For general radioactive decontamination, use a solution water before being autoclaved. Remove the rotor lid, of equal parts of 70 % ethanol, 10 % SDS and water. the centrifuge tubes and the adapters. -
Page 50: The Thermo Service Offer
Maintenance and care The Thermo Service offer Warranty conditions Thermo recommends annual servicing of the centrifuge The warranty period starts with the day of delivery. and the accessories by authorized customer service or Within the warranty period the centrifuge is repaired or trained professionals. -
Page 51: Troubleshooting
Troubleshooting Troubleshooting Mechanical emergency lid release 1. Make sure the rotor stands still. (control window). In case of a power failure you cannot open the lid nor- 2. Unplug the mains cord. mally using the electric emergency release. To permit unloading even in this case, the centrifuge is equipped 3. - Page 52 Troubleshooting 4. Gently press the centrifuge lid down to disengage the lid-latch. 5. While pressing down the lid push the wire down further until you hear and feel the lid-latch unlock- ing. Remove the auxiliary tool and open the centri- fuge lid.
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Page 53: Problems You Can Handle Yourself
Troubleshooting Problems you can handle yourself If problems other than those described in the following tables arise, you must consult your nearest authorized service. Error Symptom Possible causes and corrective measures Displays remain dark The drive stops. Mains voltage disconnection The rotor stops without 1. - Page 54 Troubleshooting Error Symptom Possible causes and corrective measures – Exceptionally running Imbalance. noise. 1. Stop the centrifuge by pressing the "stop" key, in case of emergency, unplug mains power cord. 2. Wait until the centrifuge comes to a complete stop. 3.
- Page 55 Troubleshooting Error Symptom Possible causes and corrective measures "bAL" The rotor stops with Imbalance switch releases. deceleration. 1. Open the instrument by pressing the “lid open” in case. 2. Check whether the rotor is properly loaded. 3. Check whether a broken tube or a damage to the rotor release the imbalance switch.
- Page 56 Troubleshooting Error Symptom Possible causes and corrective measures E-22/E-23 Rotor stops without Error in speed entry deceleration to standstill. Switch the instrument off and on again. Instrument cannot be If the error persists, contact service representative. operated. E-24 Instrument cannot be Wrong status information from the lid-latch.
- Page 57 Troubleshooting Error Symptom Possible causes and corrective measures E-31 Rotor stops without Overtemperature in the motor. deceleration to standstill or 1. Turn instrument off and unplug mains power cord. does not start. 2. Check and clean the venting slots if necessary and re- spectively the filter unit of the cooled centrifuge.
- Page 58 Troubleshooting Error Symptom Possible causes and corrective measures E-41 Rotor stops without Internal program error. deceleration to standstill. Switch the instrument off and on again. Instrument cannot be E-56 If the error persists, contact service representative. operated. E-60 Rotor stops with decelera- Insufficient temperature in the refrigeration unit.
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Page 59: Contacting Service
Troubleshooting Contacting Service Subsequently, the following readings will be dis- played for 5 seconds each Should you require help from your service repre- sentative, please indicate the catalog and serial SoFt Software number 0 5 8 number of your instrument. You will find the pertinent information at the specifications, near the socket for _ 0 1 Software version... - Page 60 Troubleshooting for your notes...
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Page 61: Technical Data
Technical data Technical data Component parts Part / function Description Hausing/body Sheet steel chassis with attached plastic housing and steel reinforcement Keys and display panel Keys and display panel covered with easy care protective foil Operation "Easycontrol" system Rotor chamber dimensions (D x H) (diameter x height): 190 mm x 70 mm Micro 17/21... -
Page 62: The "Easycontrol" User Interface
Technical data The „Easycontrol“ user interface Function Performance Start Start key ( Stop Stop key ( „Quick run“ key Quick starting and stopping ): short-time run when pressed permanently; stop when ( released Indication of operating state Spinning rotor indicated by rotating lights (LED) in the speed display panel Speed display reads „End“... -
Page 63: Performance
Technical data Performance Performance Value/description Micro 17R in parentheses) Micro 17 / L EGEND EGEND environmental conditions indoor use maximum elevation 2000 m above sea level maximum relative humidity 80 % up to 31 °C; linearly decreasing down to 50 % relative humidity at 40 °C. permissible temperature of the environment 5 °C to + 40 °C during operation (no condensation) -10 °C to 55 °C for storage and shipping... - Page 64 Technical data Performance Value/description dimensions (H x W x D) LEGEND icro 230 mm x 240 mm x 350 mm 17/21 330 mm x 292 mm x 440 mm Micro 17R/21R EGEND weight with rotor LEGEND icro 17/21 10.5 kg Micro 17R/21R 28.0 kg...
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Page 65: Electrical Connections
Technical data Electrical connections Nominal Power Order no. Voltage Frequency Fuses inside instrument * current consumption 75002430 Legend Micro 17 230 V ±10% 1.4 A 180 W 2 x 4.0 AT 250 V (5 x 20 mm) 50 / 60 Hz 75002433 75002431 Legend Micro 17... - Page 66 Technical data Nominal Power Order no. Voltage Frequency Fuses inside instrument * current consumption 75002440 Legend Micro 17R 230 V ±10% 50 / 60 Hz 1.9 A 320 W 2 x 4.0 AT 250V (5 x 20 mm) 75002443 75002441 Legend Micro 17R 120 V ±10% 60 Hz...
- Page 67 Technical data Refrigerant Art. No. Centrifuge Refrigerant Quantity Pressure 75002440 Thermo Scientific Sorvall Legend Micro 17R R-134a 0.26 kg 21 bar 1430 0.37 t 75002441 Thermo Scientific Sorvall Legend Micro 17R R-134a 0.26 kg 21 bar 1430 0.37 t 75002442 Thermo Scientific Sorvall Legend Micro 17R R-134a 0.26 kg 21 bar...
- Page 68 Technical data For your notes...
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Page 69: Appendix
Appendix Appendix Speed / RCF diagram Speed/RCF diagram 24 x 1.5 / 2.0 mL Rotor 75003424 100000 = 8.6 cm = 14800 min RCF (r ) = 21058 = 5.1 cm 10000 1000 1000 10000 100000 speed (rpm) - Page 70 Appendix Speed/RCF diagram 36 x 0.5 mL Rotor 75003436 100000 = 14 800 min = 7.9 cm RCF (r ) = 19344 = 5.0 cm 10000 1000 1000 10000 100000 speed (min...
- Page 71 Appendix Speed/RCF diagram Dual Row 18 x 2 / 0.5 mL Rotor 75003418 100000 = 14 800 min = 8.5 cm RCF (r ) = 20813 = 4.8 cm 10000 1000 1000 10000 100000 Speed (min...
- Page 72 Appendix Speed/RCF diagram PCR 4 x 8 Rotor 75003440 100000 = 14 800 min = 6.6 cm RCF (r ) = 16161 = 4.7 cm 10000 1000 1000 10000 100000 speed (min...
- Page 73 Appendix Speed /RCF diagram PCR 8 x 8 Rotor 75003489 100000 = 14 800 min = 7.0 cm RCF (r ) = 17140 = 4.4 cm 10000 1000 1000 10000 100000 speed (min...
- Page 74 Appendix For your notes...
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Page 75: Autoclaving Protocol
Appendix Autoclaving protocol Date Remark Operator Signature... - Page 76 Appendix Autoclaving protocol Date Remark Operator Signature...
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Page 77: Index
Index Index protective tubes for corrosive substances 4 acceleration 8 acceleration time 12, 13, 14 damage aerosol-tight 12, 13, 14, 26 symbol for potential 3 aerosol-tightness dangerous chemicals 4 test 26 deceleration time 12, 13, 14 aluminum rotor: 44 decontamination 43, 45 approved rotors 28 Decontamination 45 autoclaving 45... - Page 78 Index fine tuning temperature 35 "quick run" 8 formula "start" 37 maximum permissible load 30 keys fuses 61 general operation 8 kinetic energy 59 handling the centrifuge 4 Handling the rotor 21 hazardous substances 4 blockage 49 hints Lid lock with safety check 7 symbol for 3 light rotating 37...
- Page 79 Index mechanical lid release 47 pretemp 36 problems handling of 49 protective tubes for corrosive substances 4 noise 59 noise suppression g 60 quick run function 38 quick run key 8 operation continuous 38 preselected run time 34 short-time 38 Order no.
- Page 80 Index rotor insertion software check temperature 28 internal 27 rotor wrench 39 software version rotors determination 55 approved 28 speed 32 run time permissible 30 continuous operation 34 speed, maximum 12, 13, 14, 59 range 34 start key 37 setting 34 starting run 37 stopping 38 substructure 9...
- Page 81 Index Turning the centrifuge off 40 warning signal 39 warranty conditions 46 wear 4 unbalance detection 31 weight 60 wrench for rotor insertion 28...
- Page 82 Index For your note...
- Page 83 Index For your note...
- Page 84 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/centrifuge © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc.and its subsidiaries unless otherwise specified. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As.
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Error 15 Sorvall Legend Micro 17 R
Error 15 on the ThermoFisher Scientific Sorvall Legend Micro 17 indicates a temperature measurement error. The recommended action is to switch the instrument off and on again. If the error persists, contact a service representative.
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