Preface Intended Use The centrifuge is intended for the separation of liquid human specimens, such as blood, collected in centrifugation vessels. The centrifuge is used in in-vitro diagnostic processes to support the collection of information about diseases and other physiological or pathological states, such as immunological or hematological screening (e.g.
Signal Words and Symbols Signal Word Degree of Hazard Indicates a hazardous situation that, if not avoided, could result in WAR NING death or serious injury. Indicates a hazardous situation that, if not avoided, could result in C AUTI ON minor or moderate injury.
Symbols used in the Instruction Manual Observe the information contained in the instruction manual to keep yourself and your environment safe. General hazard Electrical hazard Biological hazard Danger of cuts Hazard caused by flammable Risk of crushing materials Indicates information Hazard caused by hot considered important, but not surface.
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Risk of damage due to incorrect power supply. Make sure that the centrifuge is plugged only into a power outlet that has been properly grounded. WA RNIN G Do not use a power supply cable with inadequate rating. Risk from handling hazardous substances. When working with corrosive samples (salt solutions, acids, bases), the accessories and the centrifuge have to be cleaned thoroughly.
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Risk of contamination. Potential contaminations will not remain in the centrifuge while the device is operated. WA RNIN G Take appropriate protection measures to prevent spread of contaminations. A centrifuge is no closed containment. Damage to health from centrifuging explosive or flammable materials or substances.
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Safety can be impaired by wrong loading and worn accessories. Always make sure that the load is as equally distributed as possible. Do not use rotors and accessories which show any signs of corrosion CA UTIO N or cracks. Contact customer service for further information. Do not operate the centrifuge with an unbalanced rotor.
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To shut down the centrifuge: Press the “Stop” key. Turn off the centrifuge at the main switch. Pull out the power supply plug. In an emergency disconnect the power NOTICE supply. Make sure that the main switch and power supply plug can be accessed easily when setting up the centrifuge.
Technical Specifications 1. 2. List of Rotors Article No� Description 75003224 24 x 1.5/2.0 mL rotor with ClickSeal biocontainment lid 75003418 Dual Row 18 x 2.0/0.5 mL rotor with screw-on lid 75003436 36 x 0.5 mL rotor with screw-on lid 75003465 10 x 5 mL rotor with ClickSeal biocontainment lid 75003489...
Technical Specifications 1. 3. Technical Data Thermo Scientific MicroCL 17 Speed Range (depending on the rotor) 300–13 300 rpm RCF Value at max. Speed 17 000 x g Running Time unlimited Noise Level at max. Speed < 50 dB (A) (1 m in front of the unit at 1.6 m height) Maximum Kinetic Energy 1.9 kNm Average Heat Dissipation...
Technical Specifications Thermo Scientific MicroCL 21 Speed Range (depending on the rotor) 300–14 800 rpm RCF Value at max. Speed 21 100 x g Running Time unlimited Noise Level at max. Speed < 50 dB (A) (1 m in front of the unit at 1.6 m height) Maximum Kinetic Energy 2.37 kNm Average Heat Dissipation...
Technical Specifications Thermo Scientific MicroCL 17R Speed Range (depending on the rotor) 300–13 300 rpm RCF Value at max. Speed 17 000 x g Running Time unlimited Noise Level at max. Speed < 50 dB (A) (1 m in front of the unit at 1.6 m height) Maximum Kinetic Energy 1.9 kNm Average Heat Dissipation...
Technical Specifications Thermo Scientific MicroCL 21R Speed Range (depending on the rotor) 300–14 800 rpm RCF Value at max. Speed 21 100 x g Running Time unlimited Noise Level at max. Speed < 50 dB (A) (1 m in front of the unit at 1.6 m height) Maximum Kinetic Energy 2.37 kNm Average Heat Dissipation...
Technical Specifications 1. 3. 1. Directives and Standards Region Directive Standards Europe 98/79/EC EN 61010-1 3.1 Edition In Vitro Diagnostics EN 61010-2-020 3rd Edition (EU) 2017/746* EN 61010-2-011 2nd Edition In Vitro Diagnostics Medical Devices EN 61010-2-101 3rd Edition 2006/42/EC EN 61326-1 Class B Machinery Directive EN ISO 14971...
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Technical Specifications NOTICE: This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates, uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications.
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Technical Specifications Article Centrifuge Voltage Frequ- Rated Power Equipment Building No� ency Current Consumption Fuse Fuse Thermo Scientific 75002496 6.3 AT MicroCL Thermo 4A 2-pole Scientific 75002499 50/60 circuit MicroCL breaker Thermo 4A 2-pole Scientific 75002546 50/60 circuit MicroCL breaker Thermo Scientific 75002549...
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Technical Specifications 1. 5. Refrigerants Article Centrifuge Refrigerant Quantity Low and CO2e No� high side max� pressure Thermo 75002406 Scientific R-134a 0.26 kg 21 bar 1 430 0.37 t MicroCL 17 Thermo Scientific 75002407 R-134a 0.26 kg 21 bar 1 430 0.37 t MicroCL Thermo...
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Technical Specifications Article Centrifuge Refrigerant Quantity Low and CO2e No� high side max� pressure Thermo Scientific 75002456 R-134a 0.26 kg 21 bar 1 430 0.37 t MicroCL Thermo Scientific 75002457 R-134a 0.26 kg 21 bar 1 430 0.37 t MicroCL Thermo 75002465 Scientific...
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Technical Specifications Article Centrifuge Refrigerant Quantity Low and CO2e No� high side max� pressure Thermo Scientific 75002499 R-134a 0.26 kg 21 bar 1 430 0.37 t MicroCL Thermo Scientific 75002546 R-134a 0.26 kg 21 bar 1 430 0.37 t MicroCL Thermo 75002549 Scientific...
Technical Specifications 1. 6. Rotor Specifications 1. 6. 1. 24 x 1.5/2.0 mL Rotor Items Supplied Item Article No� Quantity 24 x 1.5/2.0 mL Rotor 75003424 O-Ring Grease 76003500 Table 10: Items Supplied 10 x 5 mL Rotor General Technical Data Maximum Permissible Load 24 x 4 g Maximum Number of Cycles...
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Technical Specifications 17R Series Centrifuges – 24 x 1�5/2�0 mL Rotor Voltage 230 V 120 V Maximum Speed 13 300 13 300 Maximum RCF-Value 17 000 17 000 Acceleration / Braking Time 10 s / 12 s 10 s / 12 s Min.
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Technical Specifications 1. 6. 2. Dual Row 18 x 2.0/0.5 mL Rotor Items Supplied Item Article No� Quantity Dual Row 18 x 2.0/0.5 mL Rotor 75003418 Table 14: Items Supplied Dual Row 18 x 2.0/0.5 mL Rotor General Technical Data Maximum Permissible Load 8 x 4 g + 8 x 0.5 g Maximum Number of Cycles...
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Technical Specifications 17R Series Centrifuges – Dual Row 18 x 2�0/0�5 mL Rotor Voltage 230 V 120 V Maximum Speed 13 300 13 300 Maximum RCF-Value 16 800 16 800 Acceleration / Braking Time 10 s / 12 s 10 s / 12 s Min.
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Technical Specifications 1. 6. 3. 36 x 0.5 mL Rotor Items Supplied Item Article No� Quantity 36 x 0.5 mL Rotor 75003436 Table 18: Items Supplied 36 x 0.5 mL Rotor General Technical Data Maximum Permissible Load 36 x 0.5 g Maximum Number of Cycles 50 000 Radius (max.
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Technical Specifications 17R Series Centrifuges – 36 x 0�5 mL Rotor Voltage 230 V 120 V Maximum Speed 13 300 13 300 Maximum RCF-Value 15 600 15 600 Acceleration / Braking Time 8 s / 10 s 8 s / 10 s Min.
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Technical Specifications 1. 6. 4. 10 x 5 mL Rotor Items Supplied Item Article No� Quantity 10 x 5 mL Rotor 75003465 O-Ring Grease 76003500 O-Ring Set 75003405 Table 22: Items Supplied 10 x 5 mL Rotor General Technical Data Maximum Permissible Load 10 x 9 g Maximum Number of Cycles...
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Technical Specifications 17R Series Centrifuges – 10 x 5 mL Rotor Voltage 230 V 120 V Maximum Speed 13 300 13 300 Maximum RCF-Value 16 414 16 414 Acceleration / Braking Time 10 s / 12 s 10 s / 12 s Min.
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Technical Specifications 1. 6. 5. PCR 8 x 8 Rotor Items Supplied Item Article No� Quantity PCR 8 x 8 Rotor 75003489 Table 26: Items Supplied PCR 8 x8 Rotor General Technical Data Maximum Permissible Load 8 x 4 g (64 x 0.5 g) Maximum Number of Cycles 50 000 Radius (max.
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Technical Specifications 17R Series Centrifuges – PCR 8 x 8 Rotor Voltage 230 V 120 V Maximum Speed 13 300 13 300 Maximum RCF-Value 13 800 13 800 Acceleration / Braking Time 6 s / 8 s 6 s / 8 s Min.
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Technical Specifications 1. 6. 6. PCR 4 x 8 Rotor Items Supplied Item Article No� Quantity PCR 4 x 8 Rotor 75003440 Table 30: Items Supplied PCR 4 x 8 Rotor General Technical Data Maximum Permissible Load 4 x 4 g (32 x 0.2 g) Maximum Number of Cycles 50 000 Radius (max.
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Technical Specifications 17R Series Centrifuges – PCR 4 x 8 Rotor Voltage 230 V 120 V Maximum Speed 13 300 13 300 Maximum RCF-Value 13 100 13 100 Acceleration / Braking Time 9 s / 12 s 9 s / 12 s Min.
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Transport and Set Up 2. Transport and Set Up The shipping carton should be inspected upon delivery. When received, carefully examine for any shipping damage before unpacking. If damage is discovered, the delivering carrier should specify and sign for the damage on your copy of the delivery receipt. Open the carton carefully making certain that all parts ("Items Supplied"...
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Transport and Set Up 2. 2. Location Operate the centrifuge only indoors. The set up location must meet these requirements: Keep a safety zone of minimum 30 cm around the centrifuge. Refer to "Safety Zone" on page Persons and hazardous substances must be kept out of this safety zone while centrifuging.
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Transport and Set Up Figure 1: Safety Zone 40 / 96...
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Transport and Set Up 2. 3. Transporting Before transporting a centrifuge make sure that the power supply cable is unplugged and removed from the centrifuge. the rotor is removed. CAUTION Damage to centrifuge or drive shaft due to movement of an installed rotor. Always remove the rotor before you transport the centrifuge.
Transport and Set Up 2. 4. Mains Connection ➀ Power Supply Switch; ➁ Mains Connection Figure 3: Mains Connection: MicroCL 17 / 21 (left); MicroCL 17R / 21R (right) Turn off the power supply switch. Make sure that the power supply cable specification agrees with the safety standards of your country.
Operation 3. Operation 3. 1. Control Panel No� Item Description Speed / RCF The speed (rpm) or RCF value (x g) is displayed here. You can modify ➀ Value the value using the up and down ARROW buttons . below. You can switch between rpm and x g using the TOGGLE button Display Mode ➁...
Operation 3. 2. Power on / off 3. 2. 1. To power on the centrifuge Push on the mains switch of the centrifuge to “1” to power it on. The centrifuge shows the actual value in the display. Speed and Time show 0. The display shows the current temperature of the sample.
Operation The acoustic signal is turned on or off. Press STOP to confirm the pre-selected value. 3. 3. Open / Close the Centrifuge Lid To open the centrifuge lid Press the Open button on the control panel. The display shows the following: To close the centrifuge lid Close the centrifuge lid by pressing down on it lightly in the middle or on both sides.
Operation 3. 4. How to Operate a Rotor The approved rotors are listed in the "List of Rotors" on page 13. Operate the centrifuge only with rotors and accessories from this list. 3. 4. 1. How to install a rotor Press the Open button control panel to open the lid of the centrifuge.
Operation If the rotor cannot be properly locked in place after several attempts, then WARN ING the rotor fixation is defective and you are not permitted to operate the rotor. Check for any damage to the rotor: Damaged rotors must not be used.
Operation Aerosol-tight Rotors When using an aerosol tight lid the rotor can be removed with the lid closed. This is to protect you and the samples. NOTICE Make sure that all components are safely fixed before you carry a rotor. 3.
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Operation a = Large seal in outer groove of rotor body; b = Small seal in groove of rotor collar Figure 5: Rotor Lid Seals CAUTION Loose parts may damage the centrifuge. When operating the rotor without the lid the seals are not fixed in their position and can damage the centrifuge.
Operation 3. 4. 4. Load the Rotor Balance Loading Load the compartments evenly. Balance opposite loads. Correct Loading Incorrect Loading Before Loading a Rotor Before loading a Rotor Inspect the rotor and all accessory parts for damage, such as cracks, scratches, or traces of corrosion.
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Operation When using an aerosol-tight rotor lid, verify that the sample tubes don’t CAU T IO N interfere with the rotor lid and don’t compromise its sealing efficiency. Tubes may open and break during centrifugation because they do not fit CAU T IO N properly to the cavities.
Operation RCF Value Explained The relative centrifugal force (RCF) is given as a multiple of the force of gravity (g). It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various centrifuges, since it is independent of the type of device. Only the centrifuging radius and the speed are used for calculation: r = centrifuging radius in cm n = rotational speed in rpm...
Operation 3. 5. Entering Centrifugation Parameters NOTICE Due to limited display digits there is a need to round the values. The direct comparison between the two values speed and RCF is therefore restricted. 3. 5. 1. Selecting Speed or RCF-Value Press the TOGGLE button to switch between the two modes.
Operation 3. 5. 3. Pre-selecting the RCF-Value Enter the desired value by pressing the up or down ARROW buttons repeatedly, until the desired value shows. You can adjust the RCF pre-selected value in steps of 100 x g. Press START to confirm the pre-selected value. If you do not press any button, the display flashes for a few seconds.
Operation Continuous Operation Press the up or down ARROW buttons until hd is shown. During continuous operation, the centrifuge will continue running until you stop it manually with STOP. CAUTION Please note that the lifetime of rotor tubes is limited, in particular if the tubes are made of plastics materials.
Operation 3. 5. 6. Pre-Tempering the Centrifugation Chamber Refrigerated centrifuges allow for pre-tempering, that is pre-warming or pre-cooling, the centrifugation chamber and the empty rotor before the centrifugation run starts. If necessary pre-temper your samples using proper equipment. The centrifuge is not intended to be used to pre-temper your samples.
Operation 3. 6. Centrifugation Damage to health from centrifuging explosive or flammable materials WARN ING or substances. Do not centrifuge explosive or flammable materials or substances. Due to air friction sample integrity may be affected. CAU T IO N The temperature of the rotor may rise significantly while the centrifuge is spinning.
Operation Continuous Operation If you select continuous operation (refer to "Continuous Operation" on page 55), you will have to stop the centrifuge manually. Press STOP on the control panel. When the message END appears in the display, press OPEN to open the centrifuge lid and remove the samples.
Operation Replacing Seals Top detail: Large seal in outer groove of rotor body; bottom detail: small seal in groove of rotor collar Lubricate all seals. Press the large seal in the outer groove of the rotor body (top detail). Press the small seal into the groove of the rotor collar (bottom detail). When loading the rotor, ensure that the rotor lid closes securely.
Operation Checking Aerosol Tightness The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in accordance with the EN 61010-2-020 Appendix AA. Whether or not a rotor is aerosol-tight depends primarily on proper handling. Check as needed to make sure your rotor is aerosol-tight. The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks, scratches and embrittlement are extremely important.
Maintenance and Care 4. Maintenance and Care 4. 1. Cleaning Intervals For the sake of personal, environmental, and material protection, you must clean and if necessary disinfect the shaker and its accessories on a regular basis. 4. 2. Basics Use warm water with a neutral detergent that is suitable for use with the materials. If ...
Maintenance and Care 4. 3. Cleaning Clean as follows: Clean rotor, buckets and accessories outside of the centrifugation chamber. Separate rotor, buckets, lids, tubes and sealing rings to allow thorough cleaning. Rinse rotor and all accessories with warm water and a neutral detergent that is suitable for use with the materials.
Maintenance and Care Cleaning the Filter Unit Refrigerated centrifuges have a filter unit to protect the cooling device. Pull the centrifuge to the edge of the table. Pull the clip below the sucking grid and remove the filter unit completely by pulling it down.
Maintenance and Care 4. 4. Disinfection You are responsible that the level of disinfection is achieved according to your requirements. After disinfection: Rinse the centrifuge and all affected accessories with water. Allow to fully drain and dry. After disinfecting, treat the entire surface of aluminum parts including the cavities with corrosion protection oil (70009824).
Maintenance and Care 4. 6. Autoclaving Always disassemble all parts before autoclaving, e.g. lids need to be removed before autoclaving a bucket or rotor. If not stated otherwise on the parts themselves, all parts can be autoclaved at 121 °C for 20 min.
Maintenance and Care 4. 9. Shipping Before shipping the centrifuge: The centrifuge must be clean and decontaminated. You must confirm the decontamination with a decontamination certificate. Before shipping the centrifuge and accessories you must clean and, if WARN ING necessary, disinfect or decontaminate the full system.
Troubleshooting 5. Troubleshooting 5. 1. Mechanical Emergency Lid Release During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency.
Troubleshooting Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Serious injuries can occur if you touch a spinning rotor with your hands or tools. A rotor can still be spinning after a power failure occurs. Do not WARN ING open the centrifuge before the rotor has stopped spinning.
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Troubleshooting Error Description Solutions Pressing OPEN has no The centrifuge Centrifuge lid is not correctly engaged or warped. lid cannot be effect. • Check if mains connection is working and the instrument is opened. switched on (display is lit). • If this is unsuccessful, you may open the centrifuge lid using the mechanical emergency lid release ("Mechanical Emergency Lid Release"...
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Troubleshooting Error Description Solutions E-22 - E-23 Rotor stops with Error in speed entry. deceleration to Switch the instrument off and on again. The display shows BR and a countdown from 100 - 0. standstill. The centrifuge cannot If the error message still shows, contact a service technician. be operated.
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Index Ice Formation 10 x 5 mL Rotor Information for the Customer Service 24 x 1.5/2.0 mL Rotor Intended Use 36 x 0.5 mL Rotor Items Supplied Aerosol-tight Applications List of Centrifuges Audible Alarm List of Rotors Autoclaving Load the Rotor Location Care Centrifugation...
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Standards Storage Technical Data Technical Specifications Transport and Set Up Troubleshooting Troubleshooting by Guide Unpacking...
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