ThermoFisher Scientific Micro SSP Gel User Manual
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ThermoFisher Scientific Micro SSP Gel User Manual

ThermoFisher Scientific Micro SSP Gel User Manual

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User Manual
Micro SSP™ Gel System
Catalog #: MGS108
TDX-OLI-DMR-PS-0619 Rev. 02

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Summary of Contents for ThermoFisher Scientific Micro SSP Gel

  • Page 1 User Manual Micro SSP™ Gel System Catalog #: MGS108 TDX-OLI-DMR-PS-0619 Rev. 02...
  • Page 2 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual MDSS GmbH Schiffgraben 41, 30175 Hannover, Germany © 2017 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

  • Page 3: Table Of Contents

    One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Table of Contents Features and Benefits ............................2 Components of the Micro SSP Gel System.....................2 Symbols.................................3 Micro SSP Gel System Preparation........................5 Electrophoresis Procedure ..........................8 Cleaning and Safety Precautions........................11 Cleaning..............................

  • Page 4: Features And Benefits

    Micro SSP Gel System User Manual Features and Benefits The Micro SSP Gel System is a simple, efficient, and compact gel box for electrophoresis. The system features: A 96-well microplate format, plus an additional lane (total of 108 sample wells) ...

  • Page 5: Symbols

    MGS-XC Symbols CAUTION. Refer to safety information in accompanying documents. If the Micro SSP Gel System equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Biological Risk TDX-OLI-DMR-PS-0619 Rev. 02...
  • Page 6 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Class II Equipment Direct Current (DC) Voltage Manufacturer Serial Number Read Usage Instructions Waste Electrical and Electronic Equipment (WEEE) Important concept for understanding and/or clarification.

  • Page 7: Micro Ssp Gel System Preparation

    One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Micro SSP Gel System Preparation CAUTION: Do not bring the Gel System into lab areas where Pre-PCR is done. Always wear PPE (Personal Protective Equipment) while handling agarose gel.
  • Page 8 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Figure 3: Dowel Holes of the Gel Box 5. Lock the Gel Box onto the Base by sliding the locking pin into the single dowel hole located on the black end of the Gel Box.
  • Page 9 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual 8. Orient to cast an agarose gel, refer to the applicable kit product insert to determine concentration and volume of agarose. Only use consumables within their expiration date. Pour the prepared agarose gel into the Gel Box, making sure the agarose covers the entire surface evenly (figure 6).

  • Page 10: Electrophoresis Procedure

    One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual 10. Once set, remove the Gel Combs by lifting the Gel Comb Holder straight up while holding the Base (figure 8). Important: Waiting too long to remove the Gel Combs can risk damaging the gel.
  • Page 11 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Important: Do not puncture the gel during sample loading. Use a multi-channel pipette and/or an 8-shooter alignment plate for increased repeatability during sample loading. Figure 10: Loading DNA samples into the wells 3.
  • Page 12 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Figure 13 & 14: Securing Cover fasteners to Base 6. Connect the red electrical lead to the voltage output plug and the black electrical lead to the black common ground plug of the power supply.

  • Page 13: Cleaning And Safety Precautions

     Do not use window sprays or kitchen scouring compounds. Power Rating The Micro SSP Gel System is designed to operate at a maximum power rating of 18W (150vDC @ 120 mA). TDX-OLI-DMR-PS-0619 Rev. 02...

  • Page 14: Electrical Safety Precautions

     Refer to the power supply manual before use.  Protect the Micro SSP Gel System and power supply from electronic surges by connecting your power supply to a surge protector.  Place the power supply close to the Cover with which it is to be connected. Be sure to place the power supply in a safe, dry location with all the controls accessible.

  • Page 15: Replacement Parts

    Micro SSP Gel System User Manual Replacement Parts CAUTION: Do not attempt to repair any component of the Micro SSP Gel System. All servicing must be done by One Lambda, A Thermo Fisher Scientific Brand. For service requests, contact Technical Support.

  • Page 16: Trouble Shooting

    Micro SSP Gel System User Manual Trouble Shooting These troubleshooting tips cover only electrophoresis-related problems using the Micro SSP Gel System. Problems related to signal intensity and quality could also be caused by sample quality/quantity, pre- PCR or other sample manipulations, or photography. Please refer to the appropriate product manuals or contact your One Lambda representative if problems persist.
  • Page 17 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Problem Possible Cause Remedy No bands Incorrect concentration or quality Use the same batch of buffer for of agarose both gel and gel running buffer.
  • Page 18 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Problem Possible Cause Remedy Faint bands Not enough Ethidium Bromide in Use fresh buffer. a gel or running buffer (Also, see the “No bands” section for other possible solutions.)
  • Page 19 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Problem Possible Cause Remedy Faint bands in the 12th row, Not enough agarose Refer to manufacturer which is the row nearest the specification. red-colored anode side of the box.
  • Page 20 One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Problem Possible Cause Remedy Air bubbles in a gel  Remove air bubbles by aspiration using a pipette or other appropriate device immediately after a gel is poured.

  • Page 21: Technical Support

    One Lambda, Inc. | A Thermo Fisher Scientific Brand Micro SSP Gel System User Manual Problem Possible Cause Remedy Hard to remove combs Agarose too cold Remove Combs after a gel has been allowed to solidify for 15 Combs not removed immediately min.

This manual is also suitable for:

Mgs108

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