Related Manuals for ThermoFisher Scientific QuantStudio 3
Summary of Contents for ThermoFisher Scientific QuantStudio 3
- Page 1 ™ QuantStudio 3 and 5 Real-Time PCR Systems INSTALLATION, USE, AND MAINTENANCE Firmware v1.2.x Publication Number MAN0010407 Revision C.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 The information in this guide is subject to change without notice. DISCLAIMER TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. Limited Use Label License No.
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Page 3: Table Of Contents
Contents About this guide ............8 Revision history . - Page 4 Contents ■ CHAPTER 3 Create and run experiments on the instrument ..28 Workflow ..............28 Options for running an experiment .
- Page 5 Contents Calibrate custom dyes ............54 Custom dyes overview .
- Page 6 Contents ■ APPENDIX A Install and connect the instrument to a network ..77 Workflow: Install and connect to a network ........77 Before you begin .
- Page 7 Contents ■ APPENDIX E Safety ........... . . 97 Symbols on this instrument .
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Page 8: About This Guide
About this guide Revision history Revision Date Description December 2015 Updates – describes new features in Firmware v1.2.x, including: • Updated workflow to create an instrument profile • Streamlined workflow for connectivity to the Thermo Fisher Cloud ™ • Improved display of VeriFlex Zones •... -
Page 9: Purpose
About this guide Purpose Purpose This guide provides information about installing, using, and maintaining the QuantStudio 3 and 5 Real-Time PCR Systems. ™ Note: For information and instructions on performing experiments on these systems, refer to the QuantStudio Design and Analysis desktop Software User Guide (Pub. no. ™... -
Page 10: Chapter 1 Product Information
Product information ■ Instrument hardware description ........10 ■... - Page 11 Chapter 1 Product information Instrument hardware description Instrument filters and supported dyes System dyes The QuantStudio 3 and 5 Systems use a coupled four-color, coupled five-color, or de- ™ coupled six-color filter set that supports the dyes shown in the following table and figure.
- Page 12 Chapter 1 Product information Instrument hardware description Custom dyes The QuantStudio 3 and 5 Systems can run assays designed with custom dyes (dyes ™ not supplied by Thermo Fisher Scientific or dyes not pre-calibrated with the instrument). Custom dyes must excite between 455 – 672 nm and emit between 505 – 723 nm.
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Page 13: Parts Of The Instrument
Chapter 1 Product information Instrument hardware description ™ Blocks with VeriFlex Zones Applied Biosystems VeriFlex technology provides independent temperature zones ™ ™ that offer enhanced functionality and precise control over your qPCR runs. ™ ™ QuantStudio 3 System QuantStudio 5 System ™... -
Page 14: Software Description
Chapter 1 Product information Software description Software description ™ Instrument, The instrument software and the QuantStudio Design and Analysis Software (desktop and cloud) include the features described below. desktop, and cloud software Feature Instrument Desktop Cloud features Use as guest (no sign in) —... -
Page 15: Folders, Templates, Experiments, And Projects
Chapter 1 Product information Software description Folders, Term Definition Supported in templates, Folders in Load Location in which you can store templates (.edt Instrument experiments, and Experiment files) on the instrument: projects • My Instrument – Displayed if you are signed in •... -
Page 16: Third-Party Software Installation
Chapter 1 Product information Network connection options Template and experiment Instrument Desktop Cloud components Plate Sample names Define and assign samples, targets or SNP assays, and tasks in the Quick Setup and Advanced Setup panes of the You cannot edit targets/SNP assays Plate tab. -
Page 17: Experiment Types
Chapter 1 Product information Experiment types Experiment types Purpose Description Standard Curve experiment Determines absolute 1. The software measures amplification of the target in a standard dilution series and in target quantity in test samples. samples. 2. The software generates a standard curve using data from the standard dilution series. 3. - Page 18 Chapter 1 Product information Experiment types Purpose Description Genotyping experiment Detects single ™ Genotyping experiments use preformulated TaqMan SNP Genotyping Assays that include: nucleotide • Two sequence-specific primers for amplification of sequences containing the SNP of polymorphism (SNP) interest. variants of a target ™...
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Page 19: Installation And Instrument Verification
Start, sign on, and configure the instrument ■ Installation and instrument verification ....... . . 19 ■... -
Page 20: Chapter 2 Start, Sign On, And Configure The Instrument
Chapter 2 Start, sign on, and configure the instrument Precautions for use Precautions for use CAUTION! PHYSICAL INJURY HAZARD. Do not remove the instrument cover. There are no components inside the instrument that you can safely service yourself. If you suspect a problem, contact technical support. CAUTION! FIRE HAZARD. -
Page 21: Parts Of The Home Screen
Chapter 2 Start, sign on, and configure the instrument Parts of the home screen Parts of the home screen Avatar and Instrument name Settings button Eject icon Buttons for accessing .edt files Help icon Connectivity icons Status dial Sign In (or My Profile) button Current user name;... - Page 22 Chapter 2 Start, sign on, and configure the instrument Parts of the home screen Element of the Function For more information, see... home screen • When the instrument is in use – Displays the sample Status dial — block temperature, the elapsed run time, and the run status.
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Page 23: Use The Instrument Without Signing In
Chapter 2 Start, sign on, and configure the instrument Use the instrument without signing in Use the instrument without signing in If the instrument is configured by an Administrator to allow guest access Settings4Manage Users4Sign In Required set to off), you can use the instrument without signing in. -
Page 24: Sign Out
Chapter 2 Start, sign on, and configure the instrument Sign out Sign out In the home screen: 1. Touch My Profile. 2. Touch Sign Out. Link your instrument profile to your Thermo Fisher Cloud account There are two ways to link an instrument profile to a Thermo Fisher Cloud account: “Link your instrument profile to Thermo Fisher Cloud“... -
Page 25: Configure Instrument Settings
Chapter 2 Start, sign on, and configure the instrument Configure instrument settings Configure instrument settings Touch Settings in the home screen to configure settings as needed. Touch for step-by-step instructions on configuring settings. Options Description Instrument Settings Instrument Name Instrument name. If connected to a network, the instrument name must be unique. (Optional) Add an avatar image for the instrument (.jpg, .png, or .gif) from a USB. - Page 26 Chapter 2 Start, sign on, and configure the instrument Configure instrument settings Options Description SAE Mode When enabled: • The home screen displays SAE mode. (Administrator only) • Runs must be started from the desktop software. The instrument locks out access ™...
- Page 27 Chapter 2 Start, sign on, and configure the instrument Configure instrument settings Options Description Run History — Displays the experiments run on the instrument and whether the data was transferred. Note: Experiments run while a user is signed-in can only be viewed or transferred by that user or an administrator.
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Page 28: Workflow
Create and run experiments on the instrument ■ Workflow ............28 ■... -
Page 29: Chapter 3 Create And Run Experiments On The Instrument
Chapter 3 Create and run experiments on the instrument Options for running an experiment Options for running an experiment Create and run an In the home screen: experiment from a 1. Touch Open Template. template 2. (Optional) Touch an experiment category in the left column. 3. -
Page 30: Repeat Your Last Experiment Run
Chapter 3 Create and run experiments on the instrument Options for running an experiment 6. (Optional) “Edit a method“ on page 32. • Add, remove, or edit a step, stage, melt curve, or data collection location. • Adjust the cover temperature, sample volume, or number of cycles •... -
Page 31: Edit An Experiment Before Starting A Run
Chapter 3 Create and run experiments on the instrument Edit an experiment before starting a run Edit an experiment before starting a run Define experiment Open an experiment (see “Run an experiment from a saved file“ on page 29) or template (see “Create and run an experiment from a template“... -
Page 32: Edit A Method
Chapter 3 Create and run experiments on the instrument Edit an experiment before starting a run To scan a barcode using the hand-held barcode scanner: 1. Select the field in the touchscreen or software where you want to enter the barcode. - Page 33 Chapter 3 Create and run experiments on the instrument Edit an experiment before starting a run Method elements A method consists of one or more stages, each with one or more steps. Within each stage, the number of times the steps repeat is indicated in the Number of Cycles field. The method also specifies the sample volume and heated cover temperature.
- Page 34 Chapter 3 Create and run experiments on the instrument Edit an experiment before starting a run • Remove a melt curve. a. Touch Remove melt curve. b. Touch on the melt curve to be removed. c. Touch Done. • Add a step. a.
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Page 35: Define Plate Wells
Chapter 3 Create and run experiments on the instrument Edit an experiment before starting a run c. Touch each zone to edit the temperature, then touch Enter. The background colors of VeriFlex Zones change with the temperature edit. ™ Zones with higher temperatures display a background brighter than those with lower temperatures. -
Page 36: Load And Unload A Plate In The Instrument
Chapter 3 Create and run experiments on the instrument Load and unload a plate in the instrument Load and unload a plate in the instrument CAUTION! Use flat caps for 0.2-mL tubes and 0.1-mL tubes. Rounded caps can damage the heated cover. 1. -
Page 37: View, Pause, Or Stop A Run
Chapter 3 Create and run experiments on the instrument View, pause, or stop a run View, pause, or stop a run During an instrument run, you can: • “View well details“ on page 37 • “Pause or stop a run“ on page 37 •... -
Page 38: Lock The Touchscreen During A Run
Chapter 3 Create and run experiments on the instrument Transfer, view, or manage files and results Lock the After you have started a run, you can lock the touchscreen so that other users cannot interfere with instrument operation. touchscreen during a run Note: You must be signed-in to use this feature. -
Page 39: Manage Template (.Edt) Files
Chapter 3 Create and run experiments on the instrument Transfer, view, or manage files and results Manage template This feature applies to experiment templates (.edt files) on your USB or Instrument. To manage completed run files (.eds files), see “Transfer experiment (.edt) files results“... -
Page 40: Chapter 4 Calibrate And Verify Instrument Performance
Calibrate and verify instrument performance ■ Calibration and verification schedule ........40 ■... -
Page 41: Calibration Descriptions
Chapter 4 Calibrate and verify instrument performance Calibration descriptions Calibration descriptions Calibration type Purpose Description Pass criteria • The image for each ROI/Uniformity The software uses calibration data The software captures a plate filter distinguishes all to map the increase in image for each optical filter. -
Page 42: View Calibration Status And Set Reminders In The Cloud
Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations c. Touch Save to save the settings or Cancel to exit the screen without saving. 5. (Optional) Touch Export to transfer the calibration report to a Cloud account, USB, or Network drive. -
Page 43: Prepare A Calibration Plate
Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations Prepare a Materials required for calibration plate preparation calibration plate • Plate(s) for the calibration you are performing: – ROI/Uniformity plate (one ROI plate needed) – Background calibration plate –... -
Page 44: Perform Calibrations
Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations Perform 1. In the home screen: calibrations Calibration type Touch ROI/Uniformity Settings4Maintenance and Service4Calibrations4ROI and Uniformity Background Settings4Maintenance and Service4Calibrations4Custom4Background Settings4Maintenance and Service4Calibrations4Dye Automatically followed by Background and Dye calibrations. 2. - Page 45 Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations 5. When the run is complete and the screen displays Calibration Complete, touch View Results to check the calibration status. Calibration status Action Passed Touch Next to proceed to the next required calibration. Failed See “Troubleshoot calibration failure“...
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Page 46: View Calibration Images And Transfer Results To Usb
Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations View calibration The instrument performs the ROI, Uniformity, and Background calibrations in sequence. You can view the calibration images after the Background calibration is images and complete. transfer results to 1. -
Page 47: Troubleshoot Calibration Failure
Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations 3. In the Calibration Status screen: a. Touch Accept Results or Reject Results. Accepting the results saves the calibration data to the instrument and overwrites existing data. b. -
Page 48: (Optional)
Chapter 4 Calibrate and verify instrument performance Perform ROI/Uniformity, Background, and Dye calibrations 3. Determine the location of the failed wells again as in step 1. If the position(s) of the Action failed well(s) are... Identical The sample block is contaminated. Decontaminate the sample block (see “Decontaminate the sample block“... -
Page 49: Perform Instrument Verification Using Rnase P Plates
Chapter 4 Calibrate and verify instrument performance Perform instrument verification using RNase P plates Perform instrument verification using RNase P plates Instruments are factory calibrated, so calibration is not necessary for initial installation. However, we recommend that you verify the instrument performance before using the instrument. -
Page 50: Analytical Performance
Chapter 4 Calibrate and verify instrument performance Perform instrument verification using RNase P plates Figure 2 384-Well RNase P plate NTC (no template control) STD 10000 copies STD 1250 copies STD 10000 copies STD 2500 copies Unknown A (5000) STD 5000 copies Unknown B (10000) Analytical After the run, the software calculates average copy number values and standard... -
Page 51: Perform Rnase P Verification
Chapter 4 Calibrate and verify instrument performance Perform instrument verification using RNase P plates 3. Vortex the plate for 5 seconds, then centrifuge for 2 minutes at 750 to 1000 × g. 4. Confirm that the liquid in each well is at the bottom of the well and free of bubbles. - Page 52 Chapter 4 Calibrate and verify instrument performance Perform instrument verification using RNase P plates 3. Touch Start. 4. When the run is complete and the screen displays Verification Complete, touch View Results to confirm the status of the run. Calibration status Action Passed Instrument is ready for use.
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Page 53: Troubleshoot Verification Failure
Chapter 4 Calibrate and verify instrument performance Perform instrument verification using RNase P plates Troubleshoot verification failure Observation Possible cause Recommended action Verification failed The plate was improperly Ensure the following: prepared. • The correct plate was used for the verification performed. -
Page 54: Calibrate Custom Dyes
Chapter 4 Calibrate and verify instrument performance Calibrate custom dyes Calibrate custom dyes Custom dyes The QuantStudio 3 and 5 Real-Time PCR Systems can be used to run assays ™ designed with custom dyes (dyes not manufactured by Thermo Fisher Scientific or overview dyes not pre-calibrated with the instrument). - Page 55 Chapter 4 Calibrate and verify instrument performance Calibrate custom dyes 1. Prepare a 2 or 3–fold dilution series of the custom dye. 2. Dispense aliquots of each dilution into the center of a reaction plate, then seal the plate. A full plate is not needed. See the figures below for suggested replicates. 3.
- Page 56 Chapter 4 Calibrate and verify instrument performance Calibrate custom dyes Run the dilution plate as an experiment 1. Load the plate into the instrument. 2. Set up a genotyping experiment on the instrument. • Load an experiment created in either the desktop or cloud software. a.
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Page 57: Calibrate The Custom Dye
Chapter 4 Calibrate and verify instrument performance Calibrate custom dyes 3. Examine the raw data and identify the well(s) yielding signals according to the ranges shown in the following table. Plate type Acceptable signal range 96-well 800,000 to 3,200,000 384-well 400,000 to 2,000,00 Note: You can also export the raw data and average for the various concentrations. - Page 58 Chapter 4 Calibrate and verify instrument performance Calibrate custom dyes 3. Enter the dye information: Field/option Action Custom Dye Enter a name for the custom dye. Name IMPORTANT! · Do not use a system dye name for a custom dye name. ·...
- Page 59 Chapter 4 Calibrate and verify instrument performance Calibrate custom dyes 9. Review the plot. Passing calibration results show uniform signals with peaks aligned with the dye's wavelength. Filter wavelength (nm) Peak channel Excitation Emission x1-m1 470 ± 15 520 ± 15 x2-m2 520 ±...
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Page 60: Calibrate For A Custom Melt Curve Experiment
Chapter 4 Calibrate and verify instrument performance Calibrate for a custom melt curve experiment Calibrate for a custom melt curve experiment Note: A custom melt calibration calibrates a custom dye and a melt calibration at the same time. Before running the custom melt calibration: •... - Page 61 Chapter 4 Calibrate and verify instrument performance Calibrate for a custom melt curve experiment 8. Review the plot. Passing calibration results show uniform signals with peaks aligned with the dye's wavelength. Note: Example dye calibration plot. The peaks for your dye may align with a different filter set. 9.
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Page 62: Chapter 5 Maintain The Instrument
Maintain the instrument ■ Backup or restore the instrument ........62 ■... -
Page 63: Decontaminate The Sample Block
Chapter 5 Maintain the instrument Decontaminate the sample block Decontaminate the sample block Perform this procedure to eliminate fluorescent contaminants from the instrument sample block. Contamination is generally evident in failed background calibrations where one or more wells consistently exhibit abnormally high signals. CAUTION! PHYSICAL INJURY HAZARD. - Page 64 Chapter 5 Maintain the instrument Decontaminate the sample block 3. Rinse the contaminated wells of the sample block with deionized water (see “Solvents for cleaning the sample block“ on page 65). 4. Close the drawer and test the block for contamination: a.
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Page 65: Solvents For Cleaning The Sample Block
Chapter 5 Maintain the instrument Replace the instrument fuses Solvents for IMPORTANT! Use these solvent cleaning procedures only in conjunction with the cleaning the "Clean the sample block" procedures. sample block Rinse the sample block with deionized water 1. Pipet a small volume of deionized water into each contaminated well. 2. -
Page 66: Replace The Fuses
Chapter 5 Maintain the instrument Power on or off, store, and move Replace the fuses 1. Power off and unplug the instrument, then allow it to cool for 15 minutes. 2. Using a flat-head screwdriver, unscrew and remove the fuse holder. 3. -
Page 67: Power On The Instrument
Chapter 5 Maintain the instrument Power on or off, store, and move Power on the To power on the instrument from a powered-off state: instrument 1. Touch anywhere on the touchscreen to determine if the instrument is in sleep mode. If the home screen displays, the instrument is already powered on. 2. -
Page 68: Move The Instrument
Chapter 5 Maintain the instrument Power on or off, store, and move Move the CAUTION! PHYSICAL INJURY HAZARD. Do not attempt to lift the instrument instrument or any other heavy objects unless you have received related training. Incorrect lifting can cause painful and sometimes permanent back injury. - Page 69 Chapter 5 Maintain the instrument Power on or off, store, and move 5. Pack the instrument in the provided packaging and follow the instructions in the table below. Prepare Include Exclude Any accessories, including: • Instrument 1. Transfer any data files from the •...
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Page 70: Chapter 6 Profile And Instrument Configuration Tasks
Profile and instrument configuration tasks ■ Initial start up ............70 ■... -
Page 71: Manage Profiles
Chapter 6 Profile and instrument configuration tasks Manage profiles Manage profiles Create a profile • “Create an administrator profile“ on page 71 • “Create a new instrument profile“ on page 23 Configure a profile • “Link your instrument profile to your Thermo Fisher Cloud account“ on page 24 •... -
Page 72: Edit A User Profile
Chapter 6 Profile and instrument configuration tasks Enable SAE mode (Administrator only) 3. Edit the profile. • To delete the profile, touch Delete profile4Delete. • To reset the PIN, touch Reset PIN4Reset. – The PIN will be deleted, and the user will be directed to enter a new PIN upon the next sign in. -
Page 73: Require Sign-In (Administrator Only)
Chapter 6 Profile and instrument configuration tasks Require sign-in (Administrator only) Require sign-in (Administrator only) In the home screen: 1. Touch Settings4Manage Users4Sign In Required. 2. Slide the control On or Off. 3. Touch Done. Requiring s user to sign in disables guest profiles. Enable remote instrument monitoring (Administrator only) In the home screen: 1. -
Page 74: Restore Factory Defaults
Chapter 6 Profile and instrument configuration tasks Restore factory defaults Restore factory defaults IMPORTANT! Back up the instrument before restoring factory defaults (see “Backup or restore the instrument“ on page 62). In the home screen: 1. Touch Settings4Instrument Settings4Restore Factory Defaults. 2. -
Page 75: Set Up A Wireless Connection
Chapter 6 Profile and instrument configuration tasks Select a Cloud region (Administrator only) Set up a wireless In the home screen: connection 1. Touch Settings4Instrument Settings4Network Connection4 Wireless to display a list of the available networks. 2. Select a network or touch Join other network, then enter the network password and touch Enter. -
Page 76: Set The Date And Time
Chapter 6 Profile and instrument configuration tasks Set the date and time Set the date and time In the home screen: 1. Touch Settings4Instrument Settings4Date/Time. 2. Select a time zone from the drop-down list. 3. Select a date format. a. Touch Date Format. b. -
Page 77: Workflow: Install And Connect To A Network
Install and connect the instrument to a network ■ Workflow: Install and connect to a network ......77 ■... -
Page 78: Appendix A Install And Connect The Instrument To A Network
Appendix A Install and connect the instrument to a network Before you begin Before you begin This section provides instructions for customer installation of the computer and the instrument. For installation by a field service engineer (FSE), contact Support to order a service call. -
Page 79: Power On And Follow The Startup Wizard
Appendix A Install and connect the instrument to a network Power on and follow the startup wizard Power on and follow the startup wizard 1. Power on the instrument. 2. Follow the startup wizard through the following tasks: • Accept license agreement •... - Page 80 Appendix A Install and connect the instrument to a network Connect the instrument and the computer directly or to a LAN 6. Set the Internet Protocol (TCP/IP) Properties for either DHCP or Static IP communication: Network Action configuration DHCP 1. Select Obtain an IP address automatically. 2.
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Page 81: Instrument And Computer Connections
Appendix A Install and connect the instrument to a network Instrument and computer connections Instrument and computer connections Figure 3 Instrument back panel USB ports RS232 Port – For service use only WiFi USB port – Connect USB wireless Fuse Cover adapter for wireless network access Power Switch (ordered separately) -
Page 82: Download And Install The Quantstudio ™ Design And Analysis Desktop Software
Appendix A Install and connect the instrument to a network Download and install the QuantStudio Design and Analysis desktop Software ™ ™ Download and install the QuantStudio Design and Analysis desktop Software Computer If you purchased a computer provided by Thermo Fisher Scientific, you can install the QuantStudio Design and Analysis desktop Software and use it to control the ™... - Page 83 Appendix A Install and connect the instrument to a network Download and install the QuantStudio Design and Analysis desktop Software ™ 3. Double-click setup.exe. 4. Follow the InstallShield Wizard prompts to install the software. 5. Accept the License Agreement. 6. Select Typical as the setup preference, then click Next. 7.
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Page 84: Networking
Appendix A Install and connect the instrument to a network Networking Networking IMPORTANT! This section provides general networking information. It does not provide adequate detail to integrate the instrument into all possible network architectures. Because a network may contain advanced features (such as a firewall or network domains), we recommend that you consult a network administrator before connecting the instrument to your laboratory network. -
Page 85: Control And Monitor Networked Instruments
Appendix A Install and connect the instrument to a network Networking Control and When the instrument is connected to a network: monitor • Computers on the network that are running the desktop software can control the networked instrument. Networked instruments can be controlled by only one computer at a time. - Page 86 Appendix A Install and connect the instrument to a network Networking • Confirm the uniqueness of the instrument name. – The instrument name must be unique within the subnet. The desktop software can automatically discover instruments on the link-local network. –...
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Page 87: Appendix B Troubleshooting
Troubleshooting Observation Possible cause Recommended action Inconsistent communication Instrument is configured for Ensure only one connectivity option is plugged between instrument-computer both wired and wireless into the instrument (either an Ethernet cable or wireless adapter, but not both). or instrument-Cloud network connection. -
Page 88: Kits, Consumables, And Accessories
Parts and materials ■ Kits, consumables, and accessories ........88 ■... -
Page 89: Appendix C Parts And Materials
Appendix C Parts and materials 384-well consumables Calibration or instrument verification plate Cat. no. 4432364 96-Well Region of Interest (ROI) and Background Plates (2 plates) ™ A26343 QuantStudio 3 or 5 10-Dye Spectral Calibration Kit, 96-Well 0.2-mL 96-Well 0.2-mL Spectral Calibration Plate 1 A26331 ™... -
Page 90: 96-Well Fast (0.1-Ml) Consumables
Appendix C Parts and materials 96-Well Fast (0.1-mL) consumables 96-Well Fast (0.1-mL) consumables Consumable Contents Cat. no. 4323032 ™ MicroAmp Optical 8-Cap Strip 300 strips 4358293 ™ MicroAmp Optical Fast 8-Tube Strip (0.1 mL) 125 strips 4358297 ™ MicroAmp Optical Fast Tube with Cap (0.1 mL) 1000 tubes 4379983 ™... -
Page 91: Quantstudio ™ 3 And 5 Systems Accessories
Appendix C Parts and materials QuantStudio 3 and 5 Systems accessories ™ ™ QuantStudio 3 and 5 Systems accessories Accessory Contents Cat. no. 4313950 ™ MicroAmp Multi-Removal Tool 1 tool 4330015 ™ MicroAmp Cap Installing Tool (handle style) 1 tool 4313663 ™... -
Page 92: Appendix D Instrument Specification And Layout
Instrument specification and layout ■ Configured system dimensions ......... 93 ■... -
Page 93: Configured System Dimensions
Appendix D Instrument specification and layout Configured system dimensions Configured system dimensions Allow space for the configured instrument. A typical setup is shown below (dimensions are rounded to nearest unit). 15 cm (6 in.) 27 cm (10.5 in.) 207 cm (81.5 in.) 30 cm (12 in.) QuantStudio 3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide... -
Page 94: Instrument Clearances
Appendix D Instrument specification and layout Electrical requirements Instrument During instrument setup and maintenance, it is necessary to access the back of the instrument. If the back of the instrument faces a wall, it will be necessary to have clearances enough space to rotate the instrument on the bench for access. -
Page 95: Environmental Requirements
Appendix D Instrument specification and layout Environmental requirements Environmental requirements Table 2 Environmental requirements Condition Acceptable range Installation site Indoor use only Electromagnetic Do not use this device in close proximity to sources of strong interference electromagnetic radiation (for example, unshielded intentional RF sources). - Page 96 Appendix D Instrument specification and layout Network requirements If a field service representative is to install your instrument: • If the instrument will be connected to a LAN, an active, tested network jack must be in place before the scheduled installation date. •...
- Page 97 Safety ■ Symbols on this instrument ..........98 ■...
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Page 98: Appendix E Safety
Appendix E Safety Symbols on this instrument Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information. In this document, the hazard symbol is used along with one of the following user attention words: •... -
Page 99: Conformity Symbols
Appendix E Safety Symbols on this instrument Symbol English Français Earth (ground) terminal Borne de (mise à la) terre Protective conductor terminal (main Borne de conducteur de protection ground) (mise à la terre principale) Terminal that can receive or supply Borne pouvant recevoir ou envoyer alternating current or voltage une tension ou un courant de type... -
Page 100: Safety Alerts On This Instrument
Appendix E Safety Safety alerts on this instrument Safety alerts on this instrument Additional text may be used with one of the symbols described above when more specific information is needed to avoid exposure to a hazard. See the following table for safety alerts found on the instrument. -
Page 101: Safety Information For Instruments Not Manufactured By Thermo Fisher Scientific
Appendix E Safety Safety information for instruments not manufactured by Thermo Fisher Scientif- Safety information for instruments not manufactured by Thermo Fisher Scientific Some of the accessories provided as part of the instrument system are not designed or built by Thermo Fisher Scientific. Consult the manufacturer's documentation for the information needed for the safe use of these products. -
Page 102: Physical Injury
Appendix E Safety Instrument safety Physical injury CAUTION! Moving and Lifting Injury. The instrument is to be moved and positioned only by the personnel or vendor specified in the applicable site preparation guide. Improper lifting can cause painful and permanent back injury. -
Page 103: Electrical
Appendix E Safety Safety and electromagnetic compatibility (EMC) standards Electrical WARNING! Fuse Installation. Before installing the instrument, verify that the fuses are properly installed and the fuse voltage matches the supply voltage. Replace fuses only with the type and rating specified for the unit. Improper fuses can damage the instrument wiring system and cause a fire. -
Page 104: Safety Compliance
Appendix E Safety Safety and electromagnetic compatibility (EMC) standards Safety compliance Reference Description EU Directive European Union “Low Voltage Directive” 2014/35/EU Safety requirements for electrical equipment for measurement, IEC 61010-1 control, and laboratory use – Part 1: General requirements EN 61010-1 UL 61010-1 CSA C22.2 No. -
Page 105: Environmental Design
Appendix E Safety Chemical safety Environmental Reference Description design Directive 2012/19/EU European Union “WEEE Directive” – Waste electrical and electronic equipment Directive 2011/65/EU European Union “RoHS Directive” – Restriction of hazardous substances in electrical and electronic equipment Directive 2006/66/EC European Union “Battery Directive” Chemical safety WARNING! GENERAL CHEMICAL HANDLING. -
Page 106: Biological Hazard Safety
Appendix E Safety Biological hazard safety Biological hazard safety WARNING! Potential Biohazard. Depending on the samples used on this instrument, the surface may be considered a biohazard. Use appropriate decontamination methods when working with biohazards. WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. -
Page 107: Documentation And Support
Documentation and support Related documentation Publication Document Description number ™ QuantStudio 3 and 5 Real-Time ™ MAN0010407 Describes the QuantStudio 3 and 5 PCR Systems Installation, Use, and Real-Time PCR Systems hardware Maintenance Guide and software and provides information on preparing, using, maintaining, and troubleshooting the system. -
Page 108: Obtain Information From The Help System
Documentation and support Obtain information from the Help system Publication Document Description number ™ ™ QuantStudio Design and Analysis MAN0010414 Describes the QuantStudio Design cloud Software Help and Analysis cloud Software and provides procedures for common tasks. ™ QuantStudio 3 and 5 Real-Time MAN0010405 Explains how to prepare your site to PCR Systems Site Preparation... -
Page 109: Limited Product Warranty
Documentation and support Limited product warranty Limited product warranty Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies' General Terms and Conditions of Sale found on Life Technologies' website at www.thermofisher.com/us/en/home/global/ terms-and-conditions.html. If you have any questions, please contact Life Technologies at www.thermofisher.com/support. -
Page 110: Index
Index 96-well 0.1-mL 90 96-well 0.2-mL 88 accessories 91 contamination, identify 47 add to method 34 custom dye administrator profile 71 add to the software 57 calibration plate, create 57 calibration, perform 58 dilution guidelines 54 Background calibration optimal concentration 56 create plate 48 wavelength requirements 54 description 41... - Page 111 Index Melt Curve experiment 17 method factory defaults 74 add steps 33 files, copy or delete 39 edit 32 firewall ports 85 parts of 33 fuses, replace 66 pause 34 ramp rate 34 Genotyping experiment 17 guest profile network disable 73 configurations supported 84 limitations 23 guidelines and best practices 85...
- Page 112 Index RNase P verification startup wizard 79 analytical performance 50 steps, add to method 33 plate description 49 stop run 37 plate, prepare 50 supported dyes 11 run 51 symbols, safety 98 ROI/Uniformity calibration system dyes 11 description 41 plate, prepare 43 run 44 when to perform 40 terms and conditions 109...
- Page 113 For support visit thermofisher.com/support or email techsupport@lifetech.com thermofisher.com 16 December 2015...
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