Advertisement

Thermo Scientific
Sorvall Legend XF / XFR
Centrifuge
Instruction Manual
50120960-f • 03 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Thermo Scientific Sorvall Legend XF and is the answer not in the manual?

Questions and answers

Summary of Contents for ThermoFisher Scientific Thermo Scientific Sorvall Legend XF

  • Page 1 Thermo Scientific Sorvall Legend XF / XFR Centrifuge Instruction Manual 50120960-f • 03 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96. It is marked by the following symbol:...
  • Page 3: Table Of Contents

    Table of Contents Preface ............................iii Scope of Supply ........................iii Intended Use ..........................iii Accident Prevention ........................ iv Precautions ..........................iv Chapter 1 Introduction and Description ....................1-1 Characteristics of the Sorvall Legend XF / XFR ............1-2 Technical Data ......................1-3 Directives, Standards and Guidelines ................1-4 Functions and Features ......................1-5 Mains Supply ......................1-5 Rotor Selection ......................1-6...
  • Page 4 Contents Chapter 5 System Menu .......................... 5-1 Description ......................5-3 Chapter 6 Maintenance and Care ......................6-1 Cleaning Intervalls ....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination ..................... 6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-5 Chapter 7 Trouble Shooting ........................
  • Page 5: Preface

    Preface Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty.
  • Page 6: Accident Prevention

    Accident Prevention Accident Prevention Prerequisite for the safe operation of the Sorvall Legend XF / XFR is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: •...
  • Page 7 Precautions • The lid emergency release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door Release” page 7-2). • Never use the centrifuge if parts of its cover panels are damaged or missing. •...
  • Page 8 Precautions This symbol refers to general hazards. CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.
  • Page 9 Introduction and Description Contents “Characteristics of the Sorvall Legend XF / XFR” page 1-2 • “Technical Data” page 1-3 • “Directives, Standards and Guidelines” page 1-4 • “Functions and Features” page 1-5 • “Mains Supply” page 1-5 • “Rotor Selection” page 1-6 •...
  • Page 10: Chapter 1 Introduction And Description

    Introduction and Description Characteristics of the Sorvall Legend XF / XFR Characteristics of the Sorvall Legend XF / XFR The Sorvall Legend XF / XFR is an in-vitro diagnostics device according to the In-Vitro Diagnostics Directive 98/79/EC. Several rotors with a wide range of tubes can be used. The set speed is reached within seconds.
  • Page 11: Technical Data

    Introduction and Description Technical Data Technical Data The technical data of the Sorvall Legend XF / XFR is listed in the following table. Table 1-1. Technical Data Sorvall Legend XF / XFR Feature Value Environmental conditions -Use in interior spaces -Altitudes of up to 2,000 m above sea level -max.
  • Page 12: Directives, Standards And Guidelines

    Introduction and Description Directives, Standards and Guidelines Directives, Standards and Guidelines Table 1-2. Directives, Standards and Guidelines Tension / Produced and inspected according to the following Frequency standards and guidelines 230V 50/60Hz 2006/95/EC Low Voltage Directive: EN 61010-1, 2 Edition 2006/42/EC &...
  • Page 13: Functions And Features

    Introduction and Description Functions and Features Functions and Features The following table gives an overview of the important functional and performance characteristics of the Sorvall Legend XF / XFR. Table 1-3. Functions and Features Component / Function Description / Features Structure / Housing Galvanized steel chassis with armoured plating Chamber...
  • Page 14: Rotor Selection

    Introduction and Description Rotor Selection Rotor Selection The Sorvall Legend XF / XFR is supplied without a rotor. Various rotors are available to choose from. TX-750 7500 3607 with round buckets 7500 3608 with rectangular buckets 7500 3614 incl. Microtiter carrier 7500 3617 incl.
  • Page 15 Before use Contents “Before Setting up” page 2-2 • “Transporting the Centrifuge” page 2-4 • “Location” page 2-4 • “Aligning the Centrifuge” page 2-4 • “Mains Connection” page 2-5 • “Storage” page 2-6 • Thermo Scientific Legend XF / XFR...
  • Page 16: Chapter 2 Before Use

    Before use Before Setting up Before Setting up 1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher Scientific immediately if any damage is discovered. 2. Remove the packaging. 3. Check the order for completeness (see “Scope of Supply”...
  • Page 17 Before use Setting up 3. Screw the rails onto the pallet in front of the centrifuge. 4. Remove the protectors that locate the centrifuge on the pallet. Release the castors. Use several people in order to roll the centrifuge off the pallet. 5.
  • Page 18: Transporting The Centrifuge

    Before use Transporting the Centrifuge CAUTION Fix the castors. Transporting the Centrifuge • Due to its weight (see “Technical Data” page 1-3), the centrifuge should be carried by several people. • The centrifuge can be damaged by impacts. • Transport the centrifuge upright and if at all possible in its packaging. WARNING Always remove the rotor before moving the centrifuge.
  • Page 19: Mains Connection

    Before use Mains Connection Mains Connection Main Power Mains plug Figure 2-1. Mains Connection 1. Turn off the power supply switch on the back (press "0"). 2. Plug the centrifuge into grounded electrical sockets only. 3. Check whether the cable complies with the safety standards of your country. 4.
  • Page 20: Storage

    Before use Storage Storage • Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. •...
  • Page 21: Control Panel

    Control Panel Contents “Control Panel” page 3-2 • “Keys” page 3-2 • Thermo Scientific Legend XF / XFR...
  • Page 22: Keys

    Control Panel Control Panel Control Panel The control panel contains the keys and displays of the centrifuge (only the power switch is located on the back of the device). All parameters can be selected and changed during operation. Program selection keys Display PULSE OPEN...
  • Page 23: Operation

    Operation Contents “Switch on Centrifuge” page 4-2 • “Lid Opening” page 4-2 • “Rotor Installation” page 4-2 • “Entering Parameters” page 4-4 • “Centrifugation” page 4-10 • “Temperature Adaptation during Standstill” page 4-11 • “Short-term Centrifugation” page 4-11 • “Removing the Rotor” page 4-12 •...
  • Page 24: Switch On Centrifuge

    Operation Switch on Centrifuge Switch on Centrifuge 1. Turn on the power switch on the back of the device. The device performs a self-check of its software. a. When the centrifuge lid is closed the following display shows: The top line shows the speed and the time. Both lines display 0. The temperature indicator displays the current temperature inside the rotor chamber.
  • Page 25 Operation Rotor Installation CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge. The centrifuge is equipped with an AutoLock locking system. This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have to be bolted on to the centrifuge spindle.
  • Page 26: Entering Parameters

    Operation Entering Parameters 6. If available close the rotor with the rotor lid. Be sure to check all sealings before starting any aeorsol-tight applications. See the information in the rotor instruction manual. 7. The display shows: Entering Parameters Acceleration curve The Sorvall Legend XF / XFR offers you a total of 9 acceleration and 10 braking curves with which samples and gradients can be centrifuged.
  • Page 27 Operation Entering Parameters TOGGLE key to toggle between the two modes. 2. Enter the desired value using the numeric pad. The digits show in sequential order. 3. Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds. Note If an extremely low RCF value has been selected, it will be corrected automatically if the resulting speed is less than 300 rpm.
  • Page 28 Operation Entering Parameters 2. Press the TIME key repeatedly to switch over to mm:ss or Hold mode respectively. 3. Enter the desired runtime using the numeric pad. The digits show in sequential order. 4. Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds.
  • Page 29 Operation Entering Parameters SAMPLE When SAMPLE is lit, the temperature of the sample will be controlled. To set the temperature, proceed as follows: 1. Press the TEMPERATURE key in order to open the temperature selection menu. The display shows the messages "Temperature". Depending on the display mode setting, the value shown corresponds either to the sample temperature or to the inside chamber temperature.
  • Page 30 Operation Entering Parameters 5. Press the START key The rotor chamber is cooled down or heated up to the preset temperature. 6. Press the STOP key The display shows the current temperature. Bucket selection Bucket selection is only possible for swing-out rotors. The bucket code corresponds to the last four digits of the bucket catalog number.
  • Page 31 Operation Entering Parameters The default value is marked accordingly. You can either confirm the default value by pressing ENTER or enter a different radius. 2. Enter the radius using the numeric pad. The digits show in sequential order. 3. Press ENTER to confirm the new radius.
  • Page 32: Centrifugation

    Operation Centrifugation Enter the program number and confirm with ENTER key 3. Enter the passcode. If you enter the wrong password, the following message appears: The display restores the current values. Return to step 1. Centrifugation Once the rotor has been properly installed, the main switch has been turned on and the lid has been closed, you can start centrifuging.
  • Page 33: Temperature Adaptation During Standstill

    Operation Temperature Adaptation during Standstill Check the loading and start the centrifuge once again. See the information on proper loading in the rotor instruction manual. For information on troubleshooting, see section “Troubleshooting by User” page 7-3. Stopping the centrifugation program With preset running time Usually the running time is preset and you only have to wait until the centrifuge stops automatically when the preset time limit expires.
  • Page 34: Removing The Rotor

    Operation Removing the Rotor Removing the Rotor To remove the rotor, proceed as follows: 1. Open the centrifuge lid. 2. Grab the rotor handle with both hands and press against the green AutoLock key. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to tilt the rotor while doing this.
  • Page 35: Aligning The Centrifuge

    Operation Aligning the Centrifuge Aligning the Centrifuge 1. To turn off the centrifuge put the mains switch to "0". Thermo Scientific Legend XF / XFR 4-13...
  • Page 36: Chapter 5 System Menu

    System Menu Thermo Scientific Legend XF / XFR...
  • Page 37 System Menu Enter system menu? STOP START English Language 1.. 7: Deutsch Deutsch Française ENTER Español End of run beep? Italiano Nederlands ENTER к Росс ий Keypad beep? ENTER LCD powersave? ENTER STOP ____ Lid passcode? Set lid passcode ENTER Program passcodes? ENTER Auto lid open?
  • Page 38: Description

    System Menu Description Description Enter system menu To enter the system menu hold down any of the keys when switching the centrifuge on. Press the ENTER key in order to navigate through the system menu. Press the STOP key to quit the system menu. Language In order to change the language in the display, press the number next to the desired language in the diagram on the numeric pad.
  • Page 39 System Menu Description Auto lid open Press 1 (yes) on the numeric pad if the centrifuge should open after the run. Otherwise press 0 (no). Press ENTER key to confirm. Software ID Here you fine the current software version. Press ENTER key to confirm.
  • Page 40: Maintenance And Care

    Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-3 • • “Decontamination” page 6-4 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-5 Thermo Scientific Legend XF / XFR...
  • Page 41: Cleaning Intervalls

    Maintenance and Care Cleaning Intervalls Cleaning Intervalls For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis. Maintenance Recommended interval Daily or when polluted Clean rotor chamber Daily or when polluted Clean rotor Daily or when polluted...
  • Page 42: Disinfection

    Maintenance and Care Disinfection CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Clean centrifuge and accessories as follows: 1. Open the centrifuge. 2.
  • Page 43: Decontamination

    Maintenance and Care Decontamination Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 4.
  • Page 44: Autoclaving

    Maintenance and Care Autoclaving 4. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. 5. Remove the centrifuge tubes and adaptors and dispose of them or disinfect them. 6. Rinse the rotor first with ethanol and then with de-ionized water. •...
  • Page 45 Maintenance and Care Service of Thermo Fisher Scientific Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician.
  • Page 46: Trouble Shooting

    Trouble Shooting Contents “Mechanical Emergency Door Release” page 7-2 • “Troubleshooting by User” page 7-3 • “When to contact Customer Service” page 7-5 • Thermo Scientific Legend XF / XFR...
  • Page 47: Mechanical Emergency Door Release

    Trouble Shooting Mechanical Emergency Door Release Mechanical Emergency Door Release During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop.
  • Page 48: Troubleshooting By User

    Trouble Shooting Troubleshooting by User 5. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the OPEN key to have the door locks operative again. WARNING If you pull only one lever or you do not pressed the OPEN key to have the door locks operative again, the door might open while the rotor os still spinning.
  • Page 49 Trouble Shooting Troubleshooting by User Failure message Problem with centrifuge Possible causes and cures PCB overtemp. The centrifuge cannot be operated. Restart the centrifuge. If an error message appears again, inform Customer Service. The run does not start or the centrifuge runs down without being braked.
  • Page 50: When To Contact Customer Service

    Trouble Shooting When to contact Customer Service Failure message Problem with centrifuge Possible causes and cures E-28 The centrifuge cannot be operated. Error during the self-test of the centrifuge program and the electronics. The run does not start or the centrifuge runs down without being braked.
  • Page 51 Trouble Shooting When to contact Customer Service 3. Press and hold the ENTER key , until the following message is displayed: Software ID: XXXXXXXX 4. Communicate the software version to the service technician. Legend XF / XFR Thermo Scientific...
  • Page 52: Chemical Compatibility Chart

    Chemical Compatibility Chart CHEMICAL S S U - S M S - S U S S U S S - S S S S U S S S S S S 2-mercaptoethanol Acetaldehyde U U - M U U U M M - M S U - Acetone M S U U S U M S S U U S U S U U U S S U U S M M S U U...
  • Page 53 Chemical Compatibility Chart CHEMICAL U U - M M - S U U U U U U U - M S M - Benzyl Alcohol Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Cesium Acetate S S S - S S S S -...
  • Page 54 Chemical Compatibility Chart CHEMICAL S S S S S S S S S S S S - S U S S S S S S S S M S M S Ethylene Glycol Ethylene Oxide Vapor S U - S M - S S S U S U S S S U ®...
  • Page 55 Chemical Compatibility Chart CHEMICAL M S S - S S S S S S S S S S S S S S S S S S S M S S S Magnesium Chloride Mercaptoacetic Acid U S U - S M S - S M S U U U U - S U U S M S U S S S S Methyl Alcohol...
  • Page 56 Chemical Compatibility Chart CHEMICAL U S U - S U M - S U M U U U U U S U M U U S U U U M S Phenol (50%) Phosphoric Acid (10%) U U M S S S U S S S S U - S S S S S S S S S U M U S S Phosphoric Acid (conc.) U U M M -...
  • Page 57 Chemical Compatibility Chart CHEMICAL U U M S S U U - S S S U S S S M U S S S S S S M S U S Hydrogen Peroxide (10%) Hydrogen Peroxide (3%) S M S S S - S S S S S S S S M S S S S S S S S S S Xylene S S U S S S M U S U U U U U U M U M U U U S U M S U S...
  • Page 58 Index Acceleration curve ........... 4-4 Keys ..............3-2 Accident Prevention .......... iv Kundendienst ...........6-5, 7-5 Aerosol-tight Rotors ........4-12 Aligning the Centrifuge ........2-4 Autoclaving ............. 6-5 Lid Opening ............4-2 Location ............2-4 Braking curve ..........4-4 Bucket radius ..........4-8 Mains Connection ...........2-5 Bucket selection ..........
  • Page 59 Index: Safety zone ............2-4 Select Program ..........4-9 Short-term Centrifugation ......4-11 Starting centrifuge program ......4-10 Stopping the centrifugation program ..... 4-11 Storage ............2-6 Switch on Centrifuge ........4-2 Technical Data ..........1-3 Temperature Adaptation .......4-11 Transporting the Centrifuge ......2-4 Trouble Shooting ..........
  • Page 60 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Deutschland thermofisher.com/centrifuge © 2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.

Table of Contents