Page 28
Table of Contents Safety Symbols and Markings..................7 About the User Manual ....................8 Introduction to the Wellwash 4 Mk 2 ................9 Intended use ........................9 Principle of operation...................... 9 Advantages of using Wellwash 4 Mk 2 ................9 Functional Description ....................
Page 29
Keywords for web pages ....................63 13.2 Literature........................63 Appendix A: Wellwash 4 Mk 2 Quick Reference Guide..............64 Appendix B: Certificate of Decontamination ................... 65 Appendix C: Wellwash 4 Mk 2 Feedback Form ................66 Appendix D: Addresses ........................67 Notes ..........................
Page 30
List of Figures and List of Tables List of Figures Fig. 4.1 Wellwash 4 Mk 2 microplate washer..............11 Fig. 4.2 Keypad......................... 12 Fig. 5.1 Accessories included with the instrument............14 Fig. 5.2 Spare tubing ......................15 Fig. 5.3 Wash arm assembly tubing ................. 15 Fig.
1 Safety Symbols and Markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated. Safety symbols and markings used on the Wellwash 4 Mk 2 Power ON Power OFF...
2 About the User Manual This User Manual has been written for the actual user (e.g., laboratory technician) and provides information on the Thermo Scientific Wellwash 4 Mk 2 microplate strip washer, including installation and operating instructions. Read the manual in its entirety prior to operating the instrument.
3.1 Intended use The Wellwash 4 Mk 2 is a microplate washer for 96-well plates. It can be used in research or routine-test laboratories by trained personnel to wash Microtiter microplates and strips in 96-well plate format that meet the SBS standards.
Chapter 4: Functional Description 4 Functional Description The Wellwash 4 Mk 2 is designed to wash wells in an 8 x 12 microplate. It comprises a pump unit and a washer unit (Fig. 4.1), which are connected by power, air and liquid lines.
Chapter 4: Functional Description 4.1 Controls and indicators Here is a description of the controls and indicators on the Wellwash 4 Mk 2. POWER ON/OFF switch This two-position switch at the rear of the pump unit controls the electrical power to both the pump and washer units.
Table 5.1 Installation check list Tick Item Unpack the Wellwash 4 Mk 2 instrument carefully. Refer to 5.2.1. Keep the original packaging and packing material for future transportation. Check the delivery for completeness. Refer to 5.2.2. Check for damage during transport. Refer to 5.2.3.
Chapter 5: Installation Fig. 4.1 shows the pump and washer units in their entirety. Lift out the washer and pump units, removing the shaped packing pieces from around the washer, and place them on a flat level surface. To lift the instrument, put your fingers under the bottom on both sides and lift it with your back straight.
This should be taken into account when deciding where the instrument should be located and operated. The Wellwash 4 Mk 2 does not produce operating noise at a level that would be harmful. No sound level measurements are required after installation.
Chapter 5: Installation 5.3 Installation procedure Any suitably skilled person using standard tools may carry out the following procedure. 1. Rest the pump unit on its side and remove the transit screws (the Allen cap head type is painted red) using the key supplied (Fig. 5.1, item 5). Relocate them in the retaining holes provided (Fig.
Chapter 5: Installation 4. Lift the wash arm assembly (the stainless steel cover). Remove the pinch valve holder (Fig. 5.5) before use. Store the wire holder for future use. Pinch valve holder Fig. 5.5 Pinch valve holder Note: The wash heads are packed separately. Install them before use. 5.
Chapter 5: Installation 6. Slide the wash head between the holders (Fig. 5.7). Note: The wash heads will only fit into the end slots when the raised flange on the wash head is facing the front. Fig. 5.7 Sliding the wash head between the holders 5.3.1 How to install the pump unit and washer unit connections Fig.
Chapter 5: Installation 2. Connect the pressure feed from the pump on the right to the wash bottle using the black-coded tube. 3. Connect the vacuum feed tube (red) from the pump to the waste bottle (Fig. 5.9, item 2). 4.
Chapter 5: Installation 6. Remove the three (3) black plastic caps from the hose connectors and the pump control at the rear of the washer unit (Fig. 5.10). Fig. 5.10 Removing the plastic caps 7. Slide the two units together until the locating dowel and electrical connectors are firmly engaged (Fig.
Chapter 5: Installation 8. Open the wash bottle (Fig. 4.1, item 4) by rotating the cap ring, while the white plug remains stationary. Lift the cap ring and open the plug. Fill the wash bottle up to the shoulder with wash reagent and reconnect ensuring that the plug mates correctly with the bottle insert (Fig.
Chapter 5: Installation 5.3.2 Wash head height To accommodate the differences in size and volume capacity of manufacturers’ microplates, the height of the wash head is adjustable. This means that a positive meniscus can always be obtained without an unnecessary waste of wash reagent.
Chapter 5: Installation 5.3.3 Mains supply cable Warning: Never operate your instrument from a power outlet that has no ground connection. Never use a mains supply cable (cord) other than the Thermo Scientific mains supply cable designed for your region. 1.
Chapter 5: Installation second intervals until the PRIME indicator is reached. The pressure generation takes 10 seconds. The PRIME indicator continues flashing to prompt the user to prime the system. 4. Select the preprogrammed wash card for 1 wash and for the 12-way wash head.
Chapter 6: Operation 6 Operation 6.1 Operational checklist Table 6.1 Operational checklist Tick Item Fill the wash bottle with appropriate washing reagent. Select the program card and insert it. When using the programmable switch card, check the settings. Switch the instrument ON. Prime the instrument.
Chapter 6: Operation 6.2 Washing a microplate Ensure that the instrument is switched OFF. Fill the wash Open the wash bottle cap (Fig. 6.1) very carefully to release any bottle and residual pressure. Let the hissing sound end prior to opening the cap empty the all the way.
Page 52
Chapter 6: Operation Warning: If the instrument is used in a manner not specified by the manufacturer, the protection provided by the instrument may be impaired. See Section 9.1 General specifications. Caution: Handle the microplate with care to avoid any contamination of the instrument and the subsequent specimens.
Chapter 6: Operation Caution: DO NOT smoke, eat or drink while using the Wellwash 4 Mk 2. Wash your hands thoroughly after handling test plates. Observe normal laboratory procedures for handling potentially dangerous plates. Use proper protective clothing. Use disposable gloves. Ensure that the working area is well ventilated.
Chapter 6: Operation 6.4 Adding wash reagent To add wash reagent, first release the pressure from the wash bottle by carefully opening the bottle cap (see Fig. 6.1). Let the hissing sound end prior to opening the cap all the way. Fig.
Chapter 6: Operation 6.5 Program selection Choose an appropriate card for the wash routine. Plug the card into the slot located at the base to the right of the washer unit. Note: Before inserting any program card, ensure that it is clean and dry. Wiping the surfaces with a soft dry cloth will suffice.
Chapter 6: Operation Contained on the programmable switch card are four (4) rotary type and nine (9) two-way type switches each marked with their function. The setting of these switches controls the function of the washer, enabling a wide variety of protocols to be performed.
Chapter 6: Operation F1 OFF – FINAL X1 and F2 OFF – FINAL X2 Table 6.2 Number of washes after a soak period Settings Number of washes F1 OFF F2 OFF FINAL X1 F2 OFF F1 OFF FINAL X2 FINAL X1 FINAL X2 PLATE –...
Chapter 6: Operation PAUSE Set the pause between each wash cycle (Table 6.5). During the strip wash mode, the pause is between each row. During the plate wash mode, the pause is between each pass over the plate. Note: The times currently available are subject to approval and may be altered to suit individual customer requirements.
(SDS, sodium dodecyl sulfate) or soap solution. If you have spilt infectious agents on the instrument, disinfect with 70% alcohol or other disinfectant. See the Wellwash 4 Mk 2 User Manual, Section 7.3. Last of all, put the dust cover on. 6.7.2...
Chapter 7: Maintenance 7 Maintenance 7.1 Regular and preventive maintenance 7.1.1 Service contracts It is strongly recommended that this instrument be regularly maintained and serviced every twelve (12) months on a contract basis by trained service engineers of the manufacturer. This will ensure that the product is properly maintained and that it gives trouble-free service.
7.1.4 Immediate Although the Wellwash 4 Mk 2 is constructed from high-quality materials, you must immediately wipe away spilled saline solutions, solvents, acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled aqua.
Although this occurrence is very rare, our customers are urged to follow this piece of advice. Bottles are available from Thermo Fisher Scientific Oy for the Wellwash 4 Mk 2 (Cat. no. WW934/1). Spare O rings for the bottle plugs are included in the spare parts bag delivered with the instrument (see Fig.
Chapter 7: Maintenance 3. Release the cover by slightly lifting the tube and slide the cover then upwards along the tube (Fig. 7.2) Fig. 7.2 Opening the liquid trap bottle cover Thermo Scientific Wellwash ® 4 Mk 2 User Manual Rev.2.3, Cat.
Chapter 7: Maintenance 4. Pull the thick vacuum tube (with the 90° angle) out of the liquid trap bottle. Fig. 7.3 Pulling the thick tube out of the liquid trap bottle 5. Pull the thin tube out of the liquid trap bottle. Mark on the thin tube Fig.
Chapter 7: Maintenance 6. Let the red-coded tube remain connected to the bottle cap and take the bottle out, unscrew the liquid trap bottle cap and empty the bottle. Fig. 7.5 Opening the liquid trap bottle 7. Close the bottle. Make sure the end of the red-coded tube is about 2 cm deeper inside the bottle than the thin tube will be.
Chapter 7: Maintenance 7.1.8 Pinch valve Occasionally the tube passing through the pinch valve becomes permanently closed, i.e., it will not return to its normal shape when the valve opens while in use. This tends to occur after extended periods of non-operation. Release the pressure in the tubing by opening the wash bottle cap slightly.
Chapter 7: Maintenance 7.1.9 Unclogging tips The dispense/aspirate tips of the wash head may become clogged with salt deposits. To clear them, switch OFF the washer, lift up the wash arm assembly and unclog the tips using the small de-clogging tool provided (see Fig. 7.7). Push the de-clogging tool into the thin metal tube.
Chapter 7: Maintenance 7.1.10 Fuse replacement 1. Switch OFF the washer and disconnect the mains supply cable. 2. Slide the washer and pump units open, and disconnect the pressure and vacuum tubing from the washer unit. Warning: Never disconnect the pressure tubing without first releasing the pressure by slightly opening the wash bottle cap.
Chapter 7: Maintenance 6. Ensure that the replacement has the same rating as appears on the rating label on the rear panel of the washer unit. Only use fuses specified by Thermo Fisher Scientific Oy. 7. Insert the new fuse to the fuse holder and push the holder into its place. Turn the holder clockwise about 45°...
Chapter 7: Maintenance Note: Ensure that only fuses with the required rated current and of the specified type are used for replacement. The use of makeshift fuses and the short-circuiting of fuse holders are prohibited. 7.2 Disposal of materials When disposing of used material, follow the Good Laboratory Practices. Refer to local regulations for the disposal of infectious material.
Chapter 7: Maintenance 2. Prime the unit with distilled water from the wash bottle. 3. Lift the wash head and fill the prime tray with glutaraldehyde solution. 4. Aspirate the prime tray using the wash head, and then reprime the prime tray with distilled water.
7.4.1 Packing The Wellwash 4 Mk 2 comprises two units that are packed in a single box. For packing the washer and pump units, follow the procedure below: 1. Place the box on the floor and open the top. Put the bottom part of the package shock absorber at the bottom of the box.
Chapter 8: Troubleshooting 8 Troubleshooting Warning: DO NOT use the instrument if it appears that it does not function properly. 8.1 Troubleshooting guide The problems covered below in Table 8.1 are considered as faults that require repair or corrective repair. If the installation procedure is carefully followed, no faults should arise.
Chapter 8: Troubleshooting Table 8.1 Troubleshooting list Error Cause Action No POWER ON indicator Mains lead not connected Connect the lead to an appropriate supply. Fuse blown Replace the fuse. The pump unit POWER Switch the POWER switch switch is OFF VAC FAIL illuminated Wash bottle is full Empty the waste bottle.
Page 75
Chapter 8: Troubleshooting Wash arm assembly not Contact authorized service. moving as it should 10 Plate carrier not moving Obstacle on the plate Keep the slideway clean. as it should carrier slideway 11 Wash cycle does not Program card not inserted Push the program card all correspond to the all the way...
UV radiation. Although this occurrence is very rare, our customers are urged to follow this piece of advice. Bottles are available from Thermo Fisher Scientific Oy for the Wellwash 4 Mk 2 (Cat. no. WW934/1). See Chapter 10 Ordering Information.
Chapter 8: Troubleshooting Ensure that only fuses with the required rated current and of the specified type are used for replacement. The use of makeshift fuses and the short-circuiting of fuse holders are prohibited. Warning: When the instrument is connected to the mains supply, the opening of covers or the removal of components is likely to expose live parts.
Chapter 8: Troubleshooting 8.4 Defects and abnormal stresses Whenever it is likely that the protection has been impaired, the instrument should be made inoperative and be secured against any unintended operation. The protection is likely to be impaired if, for example, the instrument: 1.
Chapter 9: Technical Specifications 9 Technical Specifications 9.1 General specifications General specifications Overall dimensions Washer unit Pump unit 41 cm x 22 cm x 12 cm 30 cm x 18 cm x 16 cm [16.1" x 8.7" x 4.7"] [11.8" x 7.1" x 6.3"] Weight Washer unit Pump unit...
Type 006 100 – 120 Vac, 200 – 240 Vac 50/60 Hz, 300 VA CE mark Wellwash 4 Mk 2 conforms to the following requirements: 2006/95/EC (Low Voltage Directive) 2004/108/EC (Electromagnetic Compatibility Directive, EMC) FCC Part 15, Subpart B/Class B...
Contact your local Thermo Fisher Scientific representative for ordering and service information. Code Instrument 5160770 Wellwash 4 Mk 2 220 – 240 V AC/50 Hz 5160772 Wellwash 4 Mk 2 110 – 120 V AC/60 Hz 10.1 List of accessories Code...
Chapter 11: Warranty Certificate 11 Warranty Certificate Thermo Fisher Scientific Microplate Instrumentation Business’s Wellwash 4Mk2 is fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of twelve (12) months from the date of delivery. Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher...
Chapter 13: Glossary and Abbreviations 13 Glossary and Abbreviations Enzyme immunoassay. An immunoassay using a color-changing enzyme-substrate system for indicating results. A diagnostic test method to measure or detect a substance using antibody-antigen reactions. European Norm. European Union. International Electrotechnical Commission. Microplate A rigid or framed polystyrene plate with microwells in different well formats (e.g., 6, 12, 24, 48, 96, 384, 864, etc., wells) for ease of use in performing multiple tests...
Appendix A: Wellwash 4 Mk 2 Quick Reference Guide Appendix A: Wellwash 4 Mk 2 Quick Reference Guide Ensure that the instrument is switched OFF. Fill the wash Open the wash bottle cap (Fig. 6.1) very carefully to release any bottle and residual pressure.
Appendix B: Certificate of Decontamination Appendix B: Certificate of Decontamination The decontamination procedure is required prior to shipping the instrument to Thermo Fisher Scientific Oy, e.g., for repair. If, for any reason, the instrument is shipped back to Thermo Fisher Scientific Oy, it must be accompanied by a dated and signed Certificate of Decontamination, which must be attached to the outside of the package containing the instrument.
Appendix C: Wellwash 4 Mk 2 Feedback Form Please send to Thermo Fisher Scientific Oy Fax: +358-9-32910415 Appendix C: Wellwash 4 Mk 2 Feedback Form Cat. no. Serial no. PURCHASED BY PURCHASED FROM Company/Organization Distributor Department Address Address Tel. Tel.
Appendix D: Addresses Appendix D: Addresses For the latest information on products and services, visit our worldwide web sites on the Internet at: http://www.thermo.com Manufactured by: Distributed by: Thermo Fisher Scientific Oy P.O. Box 100, FI-01621 Vantaa, Finland Tel. +358-9-329 100, Fax +358-9-3291 0415 www.thermo.com Thermo Scientific Wellwash ®...
Page 114
Attention! in all WW 4 mk2 units the vacuum fail switch is in the back of the pump unit. You can adjust it by inserting a Phillips screw driver into white plastic hole in bottom left corner at the back of the pump unit.
Page 120
WELLWASH 4 / 4Mk2 SERVICE MANUAL Spare parts Wellwash 4 / 4Mk2 Document Release Page 004/006-100-X...
Page 121
WELLWASH 4 / 4Mk2 SERVICE MANUAL Spare parts Contents SUBJECT PAGE Cover page ........................Contents ......................... Main parts ........................Washer track assembly....................Control assembly ......................Pump unit assembly ....................... Miscellaneous ........................ Accessories ........................NOTE! All Spare parts that are considered as Recommended Spare parts to service offices have in theyre stock are marked with *.
WELLWASH 4 / 4Mk2 SERVICE MANUAL Spare parts Accessories ITEM CAT. 12-way Wash Head ..................WW004/1 12-way Programme Card (1 and 4 washes), ..........WW006/2L2 2) 12-way Programme Card (1 and 4 washes), for ww4mk2......WW006/2C1 3) 12-way Programme Card (2 and 3 washes), Denley........
Page 131
WELLWASH 4 / 4Mk2 SERVICE MANUAL 2) Used in instruments manufactured after the serial number 004-078 3) For WW4mk2 4) For instruments made after s/n 006-9-4693 Document Release Page 004/006-100-X...
Need help?
Do you have a question about the Wellwash 4 and is the answer not in the manual?
Questions and answers