Agilent Technologies 1200 Series User Manual page 22

2 Getting started
Pump auxiliary (continued)
3 Minimum Stroke
4 Compressibility
22
The minimum stroke defines the volume of mobile phase
displaced by one stroke of piston 1. The stroke volume of the
pump influences the mixing performance and gradient
linearity.
The stroke of each channel is based on the flow rate per
channel, not on the total flow.
If you select a minimum stroke that is smaller than the one
that would be used by the pump operating in AUTO mode,
the pump will use the higher stroke volume.
The compressibility of the mobile phase has an effect on the
performance of the pump. For best flow accuracy and mixing
performance you can set the parameter according to the
mobile phase being used.
The default value is 50 × 10
for channel B. Always set the compressibility value for both
channels.
Limits: 0 through 150.
Solvent Compressibility (10
The compressibility parameter is stored in your method.
For more information see "Hints to Optimize the
Compressibility Compensation Setting"
-6
for channel A and 115 ×
-6
per bar).
on page 47
1200 Series Nano Pump User Manual
AUTO (default):
The pump selects a stroke
volume based on the set flow
rate.
Between 20 and 100 µl:
• 20 µl for best gradient.
• 100 µl for a longer seal
lifetime.
Acetone 126
Acetonitrile 115
Benzene 95
Carbon tetrachloride 110
Chloroform 100
-6
10 Cyclohexane 118
Ethanol 114
Ethyl acetate 104
Heptane 120
Hexane 150
Isobutanol 100
Isopropanol (2-Propanol) 100
Methanol 120
1-Propanol 100
Toluene 87
Water 46