Hints For Successful Use Of The Column And Switching Valve Compartment - Agilent Technologies 1200 Series User Manual

Hints for Successful Use of the Column and Switching Valve
Compartment
• When using the Direct Injection Mode, i.e. one column and one pump, the
• When two dimensional (2-D Mode) chromatographic separations are
1200 Series Nano Pump User Manual
column compartment which also contains the six port micro switching
valve, is un-used. The nano LC column (analytical column) is housed within
the Nanospray ion source chamber via the Nanospray needle holder.
required, the column compartment is used to house either or both the
trapping column and the ion exchange columns. The column compartment
also houses the 6-port micro switching valve necessary to switch and
control the direction of flow of the mobile phases during multi-dimensional
separations using two pumps and either two or three columns.
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