Hints To Optimize The Compressibility Compensation Setting - Agilent Technologies 1200 Series User Manual

Hints to Optimize the Compressibility Compensation Setting

The compressibility compensation default settings are 50 × 10
most aqueous solutions) for pump head A and 115 × 10
solvents) for pump head B. The settings represent average values for aqueous
solvents (A side) and organic solvents (B side). Therefore it is always
recommended to use the aqueous solvent on the A side of the pump and the
organic solvent on the B side. Under normal conditions the default settings
reduce the pressure pulsation to values (below 1 % of system pressure) that
will be sufficient for most applications. If the compressibility values for the
solvents used differ from the default settings, it is recommended to change the
compressibility values accordingly. Compressibility settings can be optimized
by using the values for various solvents described in
solvent in use is not listed in the compressibility table, when using premixed
solvents and if the default settings are not sufficient for your application the
following procedure can be used to optimize the compressibility settings:
Use the nano pump in the Normal Mode at least at 100 µl/min.
N O T E
1 Start channel A of the nano pump with the adequate flow rate. The system
2 Before starting the optimization procedure, the flow must be stable. Use
3 Your pump must be connected to a ChemStation or handheld controller, the
4 Start the recording device with the plot mode.
1200 Series Nano Pump User Manual
pressure must be between 50 and 250 bar.
degassed solvent only. Check the tightness of the system with the pressure
test.
pressure and%-ripple can be monitored with one of these instruments,
otherwise connect a signal cable between the pressure output of the pump
and a recording device (for example, 339X integrator) and set parameters.
Zero 50 %
3
Att 2
Chart Speed 10 cm/min
Becoming an expert
-6
/bar (best for
-6
/bar (to suit organic
Table 4 on page 48. If the
3
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