Related Manuals for Okolab H301-ALPHA3-SMALL
Summary of Contents for Okolab H301-ALPHA3-SMALL
- Page 1 User Manual H301-ALPHA3-SMALL REV 02 H301-ALPHA3-SMALL Compatible with the following microscope Compatible with the following Okolab Controllers • • PhaseView Alpha Light Sheet microscope Bold Line • IST-2016 _ REV01 www.oko-lab.com...
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Page 2: Table Of Contents
User Manual H301-ALPHA3-SMALL REV 02 Index Components and dimensions....................3 Insertion of the Sample Feedback Temperature Sensor ..............4 Connection of the Gas Supply ....................4 Working with perfusion ......................5 Installing the H301-ALPHA3-SMALL around the sample holder ............6 www.oko-lab.com... -
Page 3: Components And Dimensions
H301-ALPHA3-SMALL includes the following components: • Objective Collar, compatible with water immersion objective 21 mm diameter. • Sample Arm Cover • Chamber Lid • Chamber Base Objective Collar Sample Arm Cover Chamber Lid Chamber Base Figure 1. H301-ALPHA3-SMALL – Components and dimensions. www.oko-lab.com... -
Page 4: Insertion Of The Sample Feedback Temperature Sensor
A single silicon tubing carries output gas from the Okolab Gas Controller to H301-ALPHA3-SMALL. Silicon tubing con- nects to a gas input - brass opening - located on a corner of the H301-ALPHA3-SMALL (see Figure 3). Connect by gently pushing silicon tubing onto brass opening. -
Page 5: Working With Perfusion
REV 02 4. Working with perfusion The Chamber H301-ALPHA3-SMALL features 2 perfusion holes on the sides for the insertion of perfusion tubing up to 1 mm in outer diameter. Follow the instruction below to work with perfusion: Use the supplied Stainless Steel Perfusion Tubes. Attach a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to the Stainless Steel Perfusion Tubes (see image 1 in Figure 4). -
Page 6: Installing The H301-Alpha3-Small Around The Sample Holder
5. Installing the H301-ALPHA3-SMALL around the sample holder To install the H301-ALPHA3-SMALL on the stage, please follow the steps below: Insert the chamber base by sliding into place as shown in image 1 and 2 in Figure 5. Note: Please pay attention to the sample holder glass. - Page 7 User Manual H301-ALPHA3-SMALL REV 02 Figure 5. Connection of the Chamber with Stage H301-ALPHA3-SMALL_User Manual Rev 02_Published in September 2020 www.oko-lab.com...