Okolab H201-NIKON-TI-S-ER User Manual
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Okolab H201-NIKON-TI-S-ER User Manual

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H201-NIKON-TI-S-ER
Compatible with the following XY stages
•
Nikon: Ti-S-E and Ti-S-ER
IST450_rev01
www.oko-lab.com
H201-NIKON-TI-S-ER
Compatible with the following Okolab gas modules
•
•
•
•
•
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User Manual
August 2019
CO2-UNIT-BL
CO2-UNIT-BL-CP2
CO2-O2-UNIT-BL-CP2
O2-UNIT-BL [1-20]
CO2-O2-UNIT-BL [0-10;1-18]
CO2-O2-UNIT-BL [0-20;1-95]
CO2-O2-UNIT-BL [0-10;0-1]
2GF-MIXER
3GF-MIXER-HYPOXIA
3GF-MIXER-HYPEROXIA

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Summary of Contents for Okolab H201-NIKON-TI-S-ER

  • Page 1 User Manual H201-NIKON-TI-S-ER August 2019 H201-NIKON-TI-S-ER Compatible with the following XY stages Compatible with the following Okolab gas modules • • Nikon: Ti-S-E and Ti-S-ER CO2-UNIT-BL • CO2-UNIT-BL-CP2 • CO2-O2-UNIT-BL-CP2 • O2-UNIT-BL [1-20] • CO2-O2-UNIT-BL [0-10;1-18] • CO2-O2-UNIT-BL [0-20;1-95] •...

  • Page 2: Table Of Contents

    User Manual H201-NIKON-TI-S-ER August 2019 Index Components and dimensions....................3 Available Sample Holders ....................... 3 Available Lids ........................4 3.1 Sliding lid .......................... 5 3.2 Koehler Lid ........................6 3.3 Injection Lid ........................6 3.4 Laser Interlock Lid ....................... 7 Insertion of the Sample Feedback Temperature Sensor ..............

  • Page 3: Components And Dimensions

    Sample Holder-Order Separately Chamber Base Chamber top view Adapter Figure 1. H201-NIKON-TI-S-ER - Components and Dimensions. 2. Available Sample Holders The following sample holders are available. NOTE: Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly adding new inserts to the list.

  • Page 4: Available Lids

    Figure 2.Available sample holders. 3. Available Lids The following glass Lids are available for H201-NIKON-TI-S-ER: • H201-SLIDING-LID: For easy sample loading and pipetting – Included in code H201-NIKON-TI-S-ER. • H201-KOEHLER-LID*: Reduces chamber height to 21 mm and allows imaging under Koehler illumination. •...

  • Page 5: Sliding Lid

    Distance between focal plane and upper side of the glass lid without the chamber riser Chamber top view Figure 3. H201-NIKON-TI-S-ER (Sliding Lid comes as standard) Screws M2x4 Sliding Lid Figure 4. Assembly of the sliding lid www.oko-lab.com...

  • Page 6: Koehler Lid

    Chamber top view Figure 5.H201-NIKON-TI-S-ER + H201-KOEHLER-LID Injection Lid The Injection Lid is a glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample. NOTE: Compatible with sample holder 1x35 ONLY. The Injection Lid is placed onto the chamber (NO SCREWS or TOOLS are necessary).

  • Page 7: Laser Interlock Lid

    Chamber top view Figure 6. H201-NIKON-TI-S-ER + H201-INJECTION-LID Laser Interlock Lid The Laser Interlock Lid is a glass lid with a safety switch connecting to the laser controller. It automatically turns the laser off when the lid is lifted.

  • Page 8: Insertion Of The Sample Feedback Temperature Sensor

    H201-NIKON-TI-S-ER August 2019 4. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201-NIKON-TI-S-ER (see Figure 8, Front and 3D views). Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view...

  • Page 9: Working With 35 Or 60 Mm Petri Dish - Spacing Rings And Magnetic Locks

    User Manual H201-NIKON-TI-S-ER August 2019 6. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting holder’s height.
  • Page 10 User Manual H201-NIKON-TI-S-ER August 2019 Grainer-Petri35x10 Ibidi μ-Dish 35 mm-low BD Falcon-35x10 Willco-35x10 Corning-35x10 mm MatTek-P35G-X14-X Figure 12. Ring selection scheme for 35 mm dish. Ibidi μ-Dish 50 mm-low MatTek-P50G-X30-X BD Falcon-60x10 Grainer - Petri 60x15 Corning-60x15 mm Willco 60x15 Figure 13.

  • Page 11: Working With 1X3'' And 1X2'' Chamber Slides - Magnetic Locks

    User Manual H201-NIKON-TI-S-ER August 2019 7. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. NOTE: Magnetic locks are included with sample holder.

  • Page 12: Working With Mw Plates - Magnetic Locks And Chamber Riser

    User Manual H201-NIKON-TI-S-ER August 2019 8. Working with MW Plates - Magnetic locks and Chamber ris- Magnetic locks hold MW plates in place into sample holder illustrated in Figure 16 NOTE: MW plates holder must be ordered separately (NOT INCLUDED). Product code: H201-MW-HOLDER Figure 16.

  • Page 13: Connection Of The Gas Supply

    9. Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H201-NIKON-TI-S-ER. Silicon tubing connects to a gas input - brass opening - located on a corner of the H201-NOKON-TI-S-ER. See Figure 18. Connect by gently pushing silicon tubing onto brass opening.
  • Page 14 User Manual H201-NIKON-TI-S-ER August 2019 Perfusion dedicated opening 1.Frontal view 2.3D view Figure 20. Perfusion. Figure 21. How to access the perfusion holes. www.oko-lab.com...
  • Page 15 The MICROFLUIDIC-C-RISER is a chamber riser with 19x3 mm windows (on the two short sides) to allow insertion of microfluidic tubings. Windows are magnetically closed. Compatible with most of H201 and H301 Okolab chambers. Thickness of this chamber riser is 8mm (2mm more than default chamber riser). Requires Koehler lid and single ac- commodation specimen holders.

  • Page 16: Connection Of The Chamber With Adapter

    User Manual H201-NIKON-TI-S-ER August 2019 11. Connection of the Chamber with adapter Follow the steps shown in the images of Figure 23 and listed below in order to correctly install the chamber onto XY stage: Follow the arrows shown in Figure 23 Images 1 and 2 for placing the captive screws in the proper housings Place the chamber on the adapter as shown in Figure 23 Image 3 Use a 1.5mm metric Allen Wrench to tighten two of the four captive screws in place (A in Image 4) while keeping...

  • Page 17: Connection Of The Chamber With Xy Stage

    Screws housings are indicated with letter B in Image 4 of Figure 24. Screw M3x3 Screw M4x7 H201-NIKON-TI-S-ER to the XY stage. Figure 24. How to fix the www.oko-lab.com...

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