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User Manual H301-MIZAR-TILT REV 02 H301-MIZAR-TILT Compatible with Compatible with the following Okolab Controllers • • Mizar Tilt system UNO-T • UNO-T-H-PREMIXED • UNO-T-H-CO2 • H301-T-UNIT-BL-PLUS • Bold Line Gas controllers IST1748_REV02 www.oko-lab.com...
User Manual H301-MIZAR-TILT REV 02 Index Components and dimensions....................3 Available Light Trap ......................4 Available Magnetic Clamp ...................... 4 Optional Components ......................5 4.1 Available Sample Holders ......................5 4.2 Available Lid ........................5 Insertion of the Sample Feedback Temperature Sensor ..............6 Working with OKO-MH Sample Holder ..................
Chamber Bracket to fix the chamber to the stage Chamber Lid Distance between focal plane and Light Trap upper side of the glass lid Magnetic Clamp Chamber Base Chamber top view Chamber Bracket Figure 1. H301-MIZAR-TILT - Components and Dimensions. www.oko-lab.com...
Figure 2. Available Light Trap 3. Available Magnetic Clamp H301-MIZAR-TILT can accept standard glass slide with thickness between 1.50 up to 2 mm and also glass slide with thickness between 2.1 to 4 mm. The following magnetic clamps are available for H301-MIZAR-TILT.
REV 02 4. Optional Components 4.1 Available Sample Holders The following sample holders are available for H301-MIZAR-TILT. NOTE: Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly adding new inserts to the list.
REV 02 5. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-MIZAR-TILT (see 1 in Figure 6). To read the medium temperature and realize a self-calibration of the chamber, fix the Temperature Sensor tip with adhesive tape on the bottom of the medium volume of the sample holder.
User Manual H301-MIZAR-TILT REV 02 6. Working with MIZAR-1x22by40 Sample Holder The OKO-MH fits into the Chamber Base and it is held in place by the magnetic clamps in order to prevent movement inside the opening on the base. To insert the MIZAR-1x22by40, follow the steps shown in Figure 8 and listed below: Insert the MIZAR-1x22by40 into the Chamber Base (see image 1 in Figure 8).
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User Manual H301-MIZAR-TILT REV 02 Figure 8. Working with MIZAR-1x22by40 Holder. www.oko-lab.com...
User Manual H301-MIZAR-TILT REV 02 7. Working with 1”x3’’ chamber slide The 1”x3” chamber slide fits into the Chamber Base and it is held in place by the magnetic clamps in order to prevent movement inside the opening on the base. To insert the 1”x3” chamber slide, follow the steps shown in Figure 10 and listed below: Insert the 1”x3”...
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User Manual H301-MIZAR-TILT REV 02 Figure 10. Working with 1'' x 3'' chamber slide. www.oko-lab.com...
User Manual H301-MIZAR-TILT REV 02 8. Working with 35mm petri-dish Flat springs prevent movement of 35mm petri-dish inside the opening on the Chamber Base. To insert the MIZAR-1x35, follow the steps shown in Figure 12 and listed below: Insert the MIZAR-1x35 into the Chamber Base (see image 1 in Figure 12).
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User Manual H301-MIZAR-TILT REV 02 Figure 12. Working with 35mm petri-dish www.oko-lab.com...
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-MIZAR-TILT. Silicon tubing con- nects to a gas input - brass opening - located on rear side of the H301-MIZAR-TILT. See Figure 14. Connect by gently pushing silicon tubing onto brass opening.
11. Working with perfusion H301-MIZAR-TILT features 4 perfusion holes on the sides of the chamber base for the insertion of perfusion tubing up to 2.0 mm in outer diameter. Small screws plug the perfusion holes when not in use (Grub screws M2,5x3). Remove the small screws as necessary before introducing perfusion tubing.
User Manual H301-MIZAR-TILT REV 02 13. Cleaning • Turn the system off and pull the mains plug out the socket • Remove the lid from the chamber main body, and keep it separate from the chamber main body while the chamber cools down.