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User Manual H201-UP April 2015 H201-UP Compatible with the following XY stages Compatible with the following Okolab Controllers XY stage model and serial number must be H201-T-UNIT-BL specified in purchase order. www.oko-lab.com...
User Manual H201-UP April 2015 Index Components and dimensions....................3 Sample Holders ........................4 2.1 Available Sample Holders ....................... 4 Insertion of the Sample Feedback Temperature Sensor ..............5 Insertion of Sample Holder into Chamber ................... 5 Magnetic Locks ........................7 Working with 1x3’’...
User Manual H201-UP April 2015 1. Components and dimensions H301-UP includes the following components: Chamber base Chamber body, it owns 12 holes for the insertion of perfusion tubing up to 2.0 mm Objective adapter, the dimension and type of objective in use must be specified in the purchase order.
User Manual H201-UP April 2015 2. Sample Holders 2.1 Available Sample Holders The following sample holders are available. NOTE: Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly adding new inserts to the list.
H201-UP April 2015 3. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201-UP main body (see Figure 3, Frontal and 3D views). Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view...
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User Manual H201-UP April 2015 Remove the chamber by lifting it from workspace, as shown in Figure 5 Images 5 and 6 Lift and replace the sample holder, as shown in Figure 5 Image 8 Figure 5. Introduction of the Specimen Holder inside the Chamber Base.
User Manual H201-UP April 2015 5. Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 6 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting holder’s height.
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User Manual H201-UP April 2015 Ibidi μ-Dish35mm-low Grainer-Petri35x10 BD Falcon-35x10 Willco-35x10 Corning-35x10mm MatTek-P35G-X-14-X Figure 8. Ring selection scheme for 35 mm dish. Ibidi μ-dish 50mm-low MatTek-P50G-X-30-X BD Falcon 60x15 Grainer - Petri 60x15 Corning-60x15mm Willco60x15 Figure 9. Ring selection scheme for 60 mm dish.
User Manual H201-UP April 2015 6. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. NOTE: Magnetic locks are included with sample holder.
A single silicon tubing carries output gas from the Okolab Gas Controller to the H201-UP. Silicon tubing connects to a gas input - brass opening - located on a corner of the H201-UP. See Figure 12. Connect by gently pushing silicon tubing onto brass opening.
9. Connection of the Chamber with stage H201-UP fits on the stage. To fix the chamber on the stage, follow the steps shown in Figure 14 Place the holder on the stage and fix it by the four pan head screws M2.5x6 (the screws type depends on the working stage).