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Okolab H301-MIZAR-TILT User Manual

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USER MANUAL
H301-MIZAR-TILT
IST1748_REV06

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Summary of Contents for Okolab H301-MIZAR-TILT

  • Page 1 USER MANUAL H301-MIZAR-TILT IST1748_REV06...

  • Page 2: Table Of Contents

    Index CHAMBER COMPATIBILITY ........................1 COMPONENTS AND DIMENSIONS......................1 AVAILABLE LIGHT TRAPS ........................2 AVAILABLE MAGNETIC CLAMPS ......................2 OPTIONAL COMPONENTS ........................3 ........................... 3 VAILABLE AMPLE OLDERS ..........................3 VAILABLE CCESSORIES INSTALLATION ............................. 4 ....................4 NSTALLATION OF THE HAMBER ON THE TAGE MIZAR-1...

  • Page 3: Chamber Compatibility

    Chamber Compatibility H301-MIZAR-TILT is compatible with Mizar Tilt Systems. Components and Dimensions H301-MIZAR-TILT includes the following components: 1. Chamber Lid with embedded temperature sensor 2. Light Trap to avoid any reflected light (to be ordered separately) 3. Magnetic Clamps to snap lock the chamber slide (to be ordered separately) 4.

  • Page 4: Available Light Traps

    Figure 2. Available Light Traps. Available Magnetic Clamps H301-MIZAR-TILT can accept standard glass slide with thickness between 1.50 up to 2 mm and also glass slide with thickness between 2.1 to 4 mm. The following magnetic clamps are available for H301-MIZAR- TILT.

  • Page 5: Optional Components

    Optional Components Available Sample Holders The following sample holders are available for H301-MIZAR-TILT. Figure 4. Available Sample Holders. Available Accessories MIZAR-CUBE GL Stainless Steel Top Lid for 12.50x12.50 Mizar Imaging Cube. It helps to minimize evaporation. Figure 5. MIZAR-CUBE GL.

  • Page 6: Installation

    Installation of the Chamber on the Stage To fix the H301-MIZAR-TILT on the stage, follow the steps listed below and shown in Figure 6: 6. Fix the bracket to the SLR.300 Piezoconcept Stage with four M2.5x6 screws (see Image 1 of Figure 6).
  • Page 7 Figure 7. Base Top View dimensions. Figure 8. Working with MIZAR-1x22by40. Note ► To work with Mizar Cube Holder, the procedure is the same as MIZAR-1x22by40 Sample Holder.

  • Page 8: Working With 1"X3'' Chamber Slide

    Working with 1”x3’’ chamber slide The 1”x3” chamber slide fits into the Chamber Base and it is held in place by the magnetic clamps in order to prevent movement inside the opening on the base. To insert the 1”x3” chamber slide, follow the steps shown in Figure 10 and listed below: 1.

  • Page 9: Working With 35Mm Petri-Dish

    Figure 10. Working with 1”x3” chamber slide. Working with 35mm petri-dish Flat springs prevent movement of 35mm petri-dish inside the opening on the Chamber Base. To insert the MIZAR-1x35, follow the steps shown in Figure 12 and listed below: 1. Insert the MIZAR-1x35 into the Chamber Base (see image 1 in Figure 12). 2.
  • Page 10 Figure 11. Base Top View dimensions.

  • Page 11: Working With Mizar-Cube Gl

    Figure 12. Working with 35mm petri dish. Working with MIZAR-CUBE GL H301-MIZAR-TILT can accept Mizar Cube Holder and MIZAR-1x22by40 Sample Holder with Mizar Imaging Cube. The following glass lid is available to cover the 12.50 x 12.50 mm Mizar Imaging Cube, in order to reduce evaporation of the medium.

  • Page 12: Lid Assembly

    A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-MIZAR-TILT. Silicon tubing connects to a gas input - brass opening - located on rear side of the H301-MIZAR-TILT. See Figure 15. Connect by gently pushing silicon tubing onto brass opening.

  • Page 13: Working With Perfusion

    Figure 15. Gas Connection. Working with perfusion H301-MIZAR-TILT features 4 perfusion holes on the sides of the chamber base for the insertion of perfusion tubing up to 2.0 mm in outer diameter. Small screws plug the perfusion holes when not in use (Grub screws M2,5x3).

  • Page 14: Cleaning

    Figure 17. Insertion of the Sample Feedback Temperature Sensor. 10 Cleaning • Turn the system off and pull the mains plug out the socket • Remove the lid from the chamber main body, and keep it separate from the chamber main body while the chamber cools down.

  • Page 15: Document Revision Table

    11 Figure List Figure 1. Components and Dimensions........................1 Figure 2. Available Light Traps..........................2 Figure 3. Available Magnetic Clamps........................2 Figure 4. Available Sample Holders......................... 3 Figure 5. MIZAR-CUBE GL............................3 Figure 6. Installation of the Chamber on the Stage....................4 Figure 7.

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