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Related Manuals for Okolab H301-KEYENCE-BZX
Summary of Contents for Okolab H301-KEYENCE-BZX
- Page 1 User Manual H301-KEYENCE-BZX IST 2792_REV01...
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Page 2: Components And Dimensions
FIGURE LIST DOCUMENT REVISION TABLE Chamber Compatibility H301-KEYENCE-BZX is a heated top stage chamber designed for Keyence BZX systems with XY stage having 160x110mm opening. It can be configured with both BOLD LINE and UNO controllers Components and Dimensions H301-KEYENCE-BZX includes the following components: Lid with embedded temperature sensors, to place on the chamber body (No screws or tools are ●... - Page 3 Figure 1. H301-KEYENCE-BZX – Components and Dimensions. Available Sample Holder The following sample holders are available. Note ►Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly adding new inserts to the list.
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Page 4: Installation
Holder to image 6/12/24/48/96-well plates with immersion objectives. Figure 2. Available Sample Holders. Installation Installation of the Chamber into the XY Stage H301-KEYENCE-BZX fits into the XY stage of the Keyence BZX systems in the same way as Keyence stage holders. - Page 5 Figure 3. Connection of the Chamber with XY Stage Insertion of the Sample Holder into Chamber Base Sample holders fit into the chamber base and are held in place by magnets embedded within both the chamber and the holder. To introduce a sample holder with the proper orientation, match the red dot on the holder to the one on the chamber base, as illustrated in Figure 4.
- Page 6 Figure 4. Introduction of the Sample Holder inside the Chamber Base. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 5 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting the holder's height.
- Page 7 Figure 6. 1) Insertion of the ring, 2) insertion of the 35 mm dish, 3) insertion of the magnetic lock. Note ► Spacing rings are included with sample holders. Note ► Spacing rings do not block the petri inside its accommodation, but they are designed to center the petri with respect to the observation circular window.
- Page 8 Ibidi μ-Dish 50 mm-low MatTek-P50G-X30-X BD Falcon-60x10 Grainer - Petri 60x15 Corning-60x15 mm Willco 60x15 Figure 8. Ring selection scheme for 60 mm dish. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. Note ►...
- Page 9 Figure 10. Magnetic lock for 1'' x 2'' chamber slide. To lock the slide, push simultaneously the buttons indicated with (3). Working with MW Plates - Magnetic locks and Chamber riser Magnetic locks hold MW plates in place into sample holder illustrated in Figure 11 Figure 11.
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Page 10: Gas Connection
Gas Connection A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-KEYENCE-BZX. Silicon tubing connects to a gas input - brass opening - located on a side of the H301-KEYENCE-BZX. See Figure 13. Figure 13. Gas Input Port. - Page 11 Figure 14. Output Gas Connection to H301-KEYENCE-BZX. Working with perfusion The H301-KEYENCE-BZX features 23 perfusion holes (10 in the chamber riser, 13 in the chamber base) on the sides for the insertion of perfusion tubing up to 2.5 mm in outer diameter. Small screws plug the perfusion holes when not in use (screws plug M3x0.5 THD).
- Page 12 To read the temperature of the culture medium and realize a self-calibration of the chamber, fix the Temperature Sensor tip with adhesive tape on the bottom of the dish inside the H301-KEYENCE-BZX. For more information about the self-calibration, please refer to the User Manual of the Okolab Controller in use.
- Page 13 While cleaning the glass lid, do not apply strong force to the surface of the glass lid because it can be damaged. Figure List Figure 1. H301-KEYENCE-BZX – Components and Dimensions................3 Figure 2. Available Sample Holders......................... 4 Figure 3. Connection of the Chamber with XY Stage .................... 5 Figure 4.