Table of Contents
Related Manuals for Okolab H301-LUDL 96A602
Summary of Contents for Okolab H301-LUDL 96A602
- Page 1 User Manual H301-LUDL 96A602 Rev 03 H301-LUDL 96A602 Compatible with the following Z piezo stage Compatible with the following Okolab Controllers • • Ludl 96A602 H301-T-BL-PLUS • UNO-T • UNO-T-H-PREMIXED • UNO-T-H-CO2 www.oko-lab.com...
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Page 2: Table Of Contents
User Manual H301-LUDL 96A602 Rev 03 Index Components and dimensions....................3 Available Sample Holders ....................... 3 Available Lids ........................4 3.1 Koehler Lid ........................5 3.2 Injection Lid ........................6 3.3 Laser Interlock Lid ....................... 6 Insertion of the Sample Feedback Temperature Sensor ..............7 Insertion of Sample Holder into Chamber ................... -
Page 3: Components And Dimensions
User Manual H301-LUDL 96A602 Rev 03 1. Components and dimensions H301-LUDL 96A602 includes the following components: • Chamber main body with embedded temperature sensor • Koehler heated glass lid with Indium Tin Oxide (ITO) conductive coating and embedded temperature sensor •... -
Page 4: Available Lids
User Manual H301-LUDL 96A602 Rev 03 GS35-M #1 1x3in. chamber slide and #2 35mm Petri-dish LABTEK-35-M #1 Lab-Tek 1in.x2in. chambered cover glass and #2 35mm Petri-dish LABTEK-II-35-M #1 Lab-Tek II 1in.x2in. chambered cover glass and #2 35mm Petri-dish LABTEK-60-M #1 Lab-Tek 1in.x2in. chambered cover glass and #1 60mm Petri-dish LABTEK-II-60-M #1 Lab-Tek II 1in.x2in. -
Page 5: Koehler Lid
H301-LASER-INTERLOCK-LID*: Heated glass lid with safety switch. Connects to laser controller and automati- cally turns laser off when lid is lifted. Compatible only with sample holder 1x35 * OPTIONAL – not included with H301-LUDL 96A602 Koehler Lid The Koehler Lid is a heated glass lid and allows imaging under Koehler illumination. The Koehler Lid is placed onto the chamber (NO SCREWS or TOOLS are necessary). -
Page 6: Injection Lid
User Manual H301-LUDL 96A602 Rev 03 Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma- nent access to the sample. NOTE: Compatible with sample holder 1x35 ONLY. The Injection Lid is placed onto the chamber (NO SCREWS or TOOLS are necessary). -
Page 7: Insertion Of The Sample Feedback Temperature Sensor
Figure 5. H301-LUDL 96A902 + H301-LASER-INTERLOCK-LID 4. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-LUDL 96A602 (see Figure 6, Frontal and 3D views). Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view... -
Page 8: Insertion Of Sample Holder Into Chamber
User Manual H301-LUDL 96A602 Rev 03 5. Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold- er. To introduce a sample holder with the proper orientation, match the red dot on the holder to the one on the chamber base, as illustrated in Figure 7. -
Page 9: Working With 35 Or 60 Mm Petri Dish - Spacing Rings And Magnetic Locks
User Manual H301-LUDL 96A602 Rev 03 6. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 8 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting holder’s height. - Page 10 User Manual H301-LUDL 96A602 Rev 03 Grainer-Petri35x10 Ibidi μ-Dish 35 mm-low BD Falcon-35x10 Willco-35x10 Corning-35x10 mm MatTek-P35G-X14-X Figure 10. Ring selection scheme for 35 mm dish. Ibidi μ-Dish 50 mm-low MatTek-P50G-X30-X BD Falcon-60x10 Grainer - Petri 60x15 Corning-60x15 mm Willco 60x15 Figure 11.
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Page 11: Working With 1X3'' And 1X2'' Chamber Slides - Magnetic Locks
User Manual H301-LUDL 96A602 Rev 03 7. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. NOTE: Magnetic locks are included with sample holder. -
Page 12: Working With Mw Plates - Magnetic Locks And Chamber Riser
9. Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-LUDL 96A9602. Silicon tubing connects to a gas input - brass opening - located on a corner of the H301-LUDL 96A902. See Figure 16. Connect by gen- tly pushing silicon tubing onto brass opening. -
Page 13: Working With Perfusion
User Manual H301-LUDL 96A602 Rev 03 Figure 16.Connection with gas supply. 10. Working with Perfusion The Chamber Riser included with H301-LUDL 96A9602 features 12 perfusion holes for the insertion of perfusion tubing up to 2.5 mm in outer diameter. To lock the Chamber Riser into the base use a 1.5mm Allen key to secure the 4 GUTB2-2,5-6 screws as shown in Figure 17. - Page 14 User Manual H301-LUDL 96A602 Rev 03 Perfusion dedicated opening 1.Frontal view 2.3D view Figure 18. Perfusion. Figure 19. How to access the perfusion holes. www.oko-lab.com...
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Page 15: Microfluidic-C-Riser (Optional)
The MICROFLUIDIC-C-RISER is a chamber riser with 19x3 mm windows (on the two short sides) to allow insertion of microfluidic tubings. Windows are magnetically closed. Compatible with most of H201 and H301 Okolab chambers. Thickness of this chamber riser is 8mm (2mm more than default chamber riser). Requires Koehler lid and single ac- commodation specimen holders. -
Page 16: Connection Of The Chamber With Z Piezo Stage
Rev 03 11. Connection of the Chamber with Z piezo stage H301-LUDL 96A602 fits on the Z piezo Stage without any screws. To fix the chamber on the stage, follow the steps shown in Figure 21. Place the chamber on the stage (See Image 1 of Figure 21). -
Page 17: Cleaning
User Manual H301-LUDL 96A602 Rev 03 12. Cleaning • Turn the system off and pull the mains plug out the socket • Remove the lid from the chamber main body, and keep it separate from the chamber main body while the chamber cools down.