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Related Manuals for Okolab H301-PI-736-160x110
Summary of Contents for Okolab H301-PI-736-160x110
- Page 1 User Manual H301-PI-736-160x110 REV 03 H301-PI-736-160x110 Compatible with the following Z-Piezo stages Compatible with the following Okolab Controllers • • PI: P-736.ZRO, P-736.ZRN, P-736.ZCO, P- H301-T-BL-PLUS 736.ZCN • UNO-T • UNO-T-H-PREMIXED • UNO-T-H-CO2 www.oko-lab.com...
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Page 2: Table Of Contents
User Manual H301-PI-736-160x110 REV 03 Index Components and dimensions....................3 Available Sample Holders ....................... 3 Available Lids ........................4 3.1 Sliding lid .......................... 5 3.2 Koehler Lid ........................6 3.3 Injection Lid ........................6 3.4 Laser Interlock Lid ....................... 7 Insertion of the Sample Feedback Temperature Sensor .............. -
Page 3: Components And Dimensions
Sample Holder-Order Separately Chamber Riser Chamber top view Chamber Base Figure 1. H301-PI-736-160x110 - Components and Dimensions. 2. Available Sample Holders The following sample holders are available. NOTE: Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly adding new inserts to the list. -
Page 4: Available Lids
Open frame to image multi well plates with oil immersion objectives Figure 2.Available sample holders. 3. Available Lids The following heated glass Lids are available for H301-PI-736-160x110: • H301-SLIDING-LID: For easy sample loading and pipetting – Included in code H301-PI-736-160x110 www.oko-lab.com... -
Page 5: Sliding Lid
Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane and upper side of the glass lid without the chamber riser Chamber top view Figure 3. H301-PI-736-160x110 (Sliding Lid comes as standard) www.oko-lab.com... -
Page 6: Koehler Lid
User Manual H301-PI-736-160x110 REV 03 Screws M2x4 Sliding Lid Figure 4. Assembly of the sliding lid Koehler Lid The Koehler Lid is a heated glass lid reducing the chamber’s height to 22 mm and allowing imaging under Koehler il- lumination. The Koehler Lid is placed onto the chamber (NO SCREWS or TOOLS are necessary). -
Page 7: Laser Interlock Lid
Chamber top view Figure 7. H301-PI-736-160x100 + H301-LASER-INTERLOCK-LID 4. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-PI-736-160x110 (see Figure 8, Front and 3D views). www.oko-lab.com... -
Page 8: Insertion Of Sample Holder Into Chamber
User Manual H301-PI-736-160x110 REV 03 Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view 2.3D view Figure 8. Insertion of the temperature sensor inside the chamber. 5. Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold- er. -
Page 9: Working With 35 Or 60 Mm Petri Dish - Spacing Rings And Magnetic Locks
User Manual H301-PI-736-160x110 REV 03 6. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting holder’s height. - Page 10 User Manual H301-PI-736-160x110 REV 03 Grainer-Petri35x10 Ibidi μ-Dish 35 mm-low BD Falcon-35x10 Willco-35x10 Corning-35x10 mm MatTek-P35G-X14-X Figure 12. Ring selection scheme for 35 mm dish. Ibidi μ-Dish 50 mm-low MatTek-P50G-X30-X BD Falcon-60x10 Grainer - Petri 60x15 Corning-60x15 mm Willco 60x15 Figure 13.
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Page 11: Working With 1X3'' And 1X2'' Chamber Slides - Magnetic Locks
User Manual H301-PI-736-160x110 REV 03 7. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. NOTE: Magnetic locks are included with sample holder. -
Page 12: Working With Mw Plates - Magnetic Locks And Chamber Riser
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-PI-736-160x100. Silicon tubing connects to a gas input - brass opening - located on a corner of the H301-PI-736-160x110. See Figure 18. Connect by gently pushing silicon tubing onto brass opening. -
Page 13: Working With Perfusion
10. Working with Perfusion The Chamber Riser included with H301-PI-736-160x110 features 12 perfusion holes for the insertion of perfusion tubing up to 2.5 mm in outer diameter. To lock the Chamber Riser into the base use a 1.5mm Allen key to secure the 4 GUTB2-2,5-6 screws as shown in Figure 19. - Page 14 User Manual H301-PI-736-160x110 REV 03 Perfusion dedicated opening 1.Frontal view 2.3D view Figure 20. Perfusion. Figure 21. How to access the perfusion holes. www.oko-lab.com...
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Page 15: Microfluidic-C-Riser (Optional)
The MICROFLUIDIC-C-RISER is a chamber riser with 19x3 mm windows (on the two short sides) to allow insertion of microfluidic tubings. Windows are magnetically closed. Compatible with most of H201 and H301 Okolab chambers. Thickness of this chamber riser is 8mm (2mm more than default chamber riser). Requires Koehler lid and single ac- commodation specimen holders. -
Page 16: Connection Of The Chamber With Z Piezo
User Manual H301-PI-736-160x110 REV 03 11. Connection of the Chamber with Z piezo Follow the steps shown in the images of Figure 23 and listed below in order to correctly connect the chamber with Z piezo stage. Place the frame on the stage and tighten 2 flat Phillips head screws M3x6 (See Image 1 of Figure 23). Screws hous- ings are indicated with letter A in Image 1 of Figure 23. -
Page 17: Cleaning
User Manual H301-PI-736-160x110 REV 03 12. Cleaning • Turn the system off and pull the mains plug out the socket • Remove the lid from the chamber main body, and keep it separate from the chamber main body while the chamber cools down.