Okolab H301-NIKON-TI-S-ER User Manual
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Okolab H301-NIKON-TI-S-ER User Manual

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H301-NIKON-TI-S-ER
Compatible with the following XY stages
•
Nikon: Ti-S-E and Ti-S-ER
www.oko-lab.com
H301-NIKON-TI-S-ER
Compatible with the following Okolab Controllers
•
•
•
•
User Manual
H301-T-BL-PLUS
UNO-T
UNO-T-H-PREMIXED
UNO-T-H-CO2
REV 04
IST483_rev04

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Summary of Contents for Okolab H301-NIKON-TI-S-ER

  • Page 1 User Manual H301-NIKON-TI-S-ER REV 04 H301-NIKON-TI-S-ER Compatible with the following XY stages Compatible with the following Okolab Controllers • • Nikon: Ti-S-E and Ti-S-ER H301-T-BL-PLUS • UNO-T • UNO-T-H-PREMIXED • UNO-T-H-CO2 IST483_rev04 www.oko-lab.com...

  • Page 2: Table Of Contents

    User Manual H301-NIKON-TI-S-ER REV 04 Index Components and dimensions....................3 Available Sample Holders ....................... 3 Available Lids ........................5 3.1 Sliding lid .......................... 5 3.2 Koehler Lid ........................6 3.3 Injection Lid ........................6 3.4 Laser Interlock Lid ....................... 7 Insertion of the Sample Feedback Temperature Sensor ..............

  • Page 3: Components And Dimensions

    Sample Holder-Order Separately Chamber Riser Chamber Base Chamber top view Adapter Figure 1. H301-NIKON-TI-S-ER - Components and Dimensions. 2. Available Sample Holders The following sample holders are available. NOTE: Please contact info@oko-lab.com if you cannot find the sample holder you are looking for. We are constantly adding new inserts to the list.
  • Page 4 User Manual H301-NIKON-TI-S-ER REV 04 GS35-M #1 1x3in. chamber slide and #2 35mm Petri-dish LABTEK-35-M #1 Lab-Tek 1in.x2in. chambered cover glass and #2 35mm Petri- LABTEK-II-35-M #1 Lab-Tek II 1in.x2in. chambered cover glass and #2 35mm Petri- dish LABTEK-60-M #1 Lab-Tek 1in.x2in. chambered cover glass and #1 60mm Petri-...

  • Page 5: Available Lids

    3. Available Lids The following heated glass Lids are available for H301-NIKON-TI-S-ER: • H301-SLIDING-LID: For easy sample loading and pipetting – Included in code H301-NIKON-TI-S-ER. • H301-KOEHLER-LID*: Reduces chamber height to 22 mm and allows imaging under Koehler illumination. •...

  • Page 6: Koehler Lid

    Chamber top view Figure 5.H301-NIKON-TI-S-ER + H301-KOEHLER-LID Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma- nent access to the sample.

  • Page 7: Laser Interlock Lid

    Chamber top view Figure 7. H301-NIKON-TI-S-ER + H301-LASER-INTERLOCK-LID 4. Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301-NIKON-TI-S-ER (see Figure 8, Front and 3D views). www.oko-lab.com...

  • Page 8: Insertion Of Sample Holder Into Chamber

    User Manual H301-NIKON-TI-S-ER REV 04 Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1.Frontal view 2.3D view Figure 8. Insertion of the temperature sensor inside the chamber. 5. Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold- er.

  • Page 9: Working With 35 Or 60 Mm Petri Dish - Spacing Rings And Magnetic Locks

    User Manual H301-NIKON-TI-S-ER REV 04 6. Working with 35 or 60 mm Petri Dish – Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder. Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes. Threaded magnetic posts allow adjusting holder’s height.
  • Page 10 User Manual H301-NIKON-TI-S-ER REV 04 Grainer-Petri35x10 Ibidi μ-Dish 35 mm-low BD Falcon-35x10 Willco-35x10 Corning-35x10 mm MatTek-P35G-X14-X Figure 12. Ring selection scheme for 35 mm dish. Ibidi μ-Dish 50 mm-low MatTek-P50G-X30-X BD Falcon-60x10 Grainer - Petri 60x15 Corning-60x15 mm Willco 60x15 Figure 13.

  • Page 11: Working With 1X3'' And 1X2'' Chamber Slides - Magnetic Locks

    User Manual H301-NIKON-TI-S-ER REV 04 7. Working with 1x3’’ and 1x2’’ chamber slides - magnetic locks Magnetic locks prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder. NOTE: Magnetic locks are included with sample holder.

  • Page 12: Working With Mw Plates - Magnetic Locks And Chamber Riser

    9. Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-NIKON-TI-S-ER. Silicon tubing connects to a gas input - brass opening - located on a corner of the H301-NOKON-TI-S-ER. See Figure 18. Connect by gently pushing silicon tubing onto brass opening.

  • Page 13: Working With Perfusion

    10. Working with Perfusion The Chamber Riser included with H301-NIKON-TI-S-ER features 12 perfusion holes for the insertion of perfusion tubing up to 2.5 mm in outer diameter. To lock the Chamber Riser into the base use a 1.5mm Allen key to secure the 4 GUTB2-2,5-6 screws as shown in Figure 19.
  • Page 14 User Manual H301-NIKON-TI-S-ER REV 04 Perfusion dedicated opening 1.Frontal view 2.3D view Figure 20. Perfusion. Figure 21. How to access the perfusion holes. www.oko-lab.com...

  • Page 15: Microfluidic-C-Riser (Optional)

    The MICROFLUIDIC-C-RISER is a chamber riser with 19x3 mm windows (on the two short sides) to allow insertion of microfluidic tubings. Windows are magnetically closed. Compatible with most of H201 and H301 Okolab chambers. Thickness of this chamber riser is 8mm (2mm more than default chamber riser). Requires Koehler lid and single ac- commodation specimen holders.

  • Page 16: Connection Of The Chamber With Adapter

    User Manual H301-NIKON-TI-S-ER REV 04 11. Connection of the Chamber with adapter Follow the steps shown in the images of Figure 23 and listed below in order to correctly install the chamber onto XY stage: Follow the arrows shown in Figure 23 Images 1 and 2 for placing the captive screws in the proper housings Place the chamber on the adapter as shown in Figure 23 Image 3 Use a 1.5mm metric Allen Wrench to tighten two of the four captive screws in place (A in Image 4) while keeping...

  • Page 17: Connection Of The Chamber With Xy Stage

    Screws housings are indicated with letter B in Image 4 of Figure 24. Screw M3x3 Screw M4x7 H301-NIKON-TI-S-ER to the XY stage. Figure 24. How to fix the www.oko-lab.com...

  • Page 18: Cleaning

    User Manual H301-NIKON-TI-S-ER REV 04 13. Cleaning • Turn the system off and pull the mains plug out the socket • Remove the lid from the chamber main body, and keep it separate from the chamber main body while the chamber cools down.

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