Related Manuals for Okolab H301-ALPHA3-LARGE
Summary of Contents for Okolab H301-ALPHA3-LARGE
- Page 1 User Manual H301-ALPHA3-LARGE REV 01 H301-ALPHA3-LARGE H301-ALPHA3-LARGE is compatible with PhaseView Alpha Light Sheet microscope. IST 2068_REV01 www.oko-lab.com...
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Page 2: Table Of Contents
User Manual H301-ALPHA3-LARGE REV 01 Index Components and dimensions....................3 Installation of the Chamber around the sample holder..............4 Connection of the Gas Supply ....................6 Working with perfusion ......................6 Insertion of the Sample Feedback Temperature Sensor ..............7 Cleaning ........................... -
Page 3: Components And Dimensions
H301-ALPHA3-LARGE includes the following components: • Objective Collar, compatible with water immersion objective 21 mm diameter. • Sample Arm Cover • Chamber Lid • Chamber Base Objective Collar Sample Arm Cover Chamber Lid Chamber Base Figure 1. H301-ALPHA3-LARGE – Components and dimensions. www.oko-lab.com... -
Page 4: Installation Of The Chamber Around The Sample Holder
2. Installation of the Chamber around the sample holder. To install the H301-ALPHA3-LARGE around the sample holder, please follow the steps below: Insert the chamber base by sliding into place as shown in image 1 and 2 in Figure 2. - Page 5 User Manual H301-ALPHA3-LARGE REV 01 Figure 2. Chamber installation around the sample holder. www.oko-lab.com...
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Page 6: Connection Of The Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to H301-ALPHA3-LARGE. Silicon tubing con- nects to a gas input - brass opening - located on a corner of the H301-ALPHA3-LARGE (see Figure 3). Connect by gently pushing silicon tubing onto brass opening. -
Page 7: Insertion Of The Sample Feedback Temperature Sensor
To read the temperature of the culture medium and realize a self-calibration of the chamber, fix the Temperature Sensor tip with adhesive tape on the sample holder inside the H301-ALPHA3-LARGE. For more information about the self-calibration, please refer to the User Manual of the Okolab Controller in use. -
Page 8: Cleaning
User Manual H301-ALPHA3-LARGE REV 01 Sensor Tip Adhesive Tape Opening for Temperature Sensor 1.Frontal view 2. Temperaure Sensor placement Figure 5. Insertion of the temperature sensor inside the chamber. 6. Cleaning • Unplug all electrical cables. • Remove the chamber lid from the chamber main body, and keep it separate from the chamber base while the chamber cools down.