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Thermo Scientific ISQ 7610 User Manual

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ISQ 7610
Mass Spectrometers
User Guide
1R120621-0003 Revision A April 2022

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Summary of Contents for Thermo Scientific ISQ 7610

  • Page 1 ISQ 7610 Mass Spectrometers User Guide 1R120621-0003 Revision A April 2022...
  • Page 3 © 2022 Thermo Fisher Scientific Inc. All rights reserved. ISQ, TSQ, TRACE, TriPlus, TraceFinder, and Chromeleon are trademarks, and Xcalibur is a registered trademark of Thermo Fisher Scientific in the United States. The following are registered trademarks in the United States and other countries: Microsoft and Windows are registered trademarks of Microsoft.
  • Page 4 Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc. The contents of this document are subject to change without notice.

  • Page 5: Table Of Contents

    Chapter 3 Tuning the ISQ 7610 Mass Spectrometer ....... . .29 Accessing ISQ 7610 Tuning .
  • Page 6 AEI Diagnostics..........42 Tuning the ISQ 7610 Mass Spectrometer ......42 Using SmartTune .
  • Page 7 No Ions Present in Scans ......... 225 Checking the ISQ 7610 System Firmware......225 How to Know When Your System Needs Maintenance .
  • Page 9 • Direct Probe Controller (DPC) standards: EMC EN 61326-1:2006. Safety IEC 61010-1:2001, IEC 61010-2-081:2001 • ISQ, ISQ 7000, ISQ 7610 standards: EMC EN 61326-1:2013. Safety IEC 61010-1:2010, IEC 61010-2- 010:2014, IEC 61010-2-081:2015. • TSQ 8000, TSQ 8000 Evo, TSQ Duo, TSQ 9000, and TSQ 9610 standards: EMC EN 61326-1:2013. Safety...
  • Page 10 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one person alone to handle safely.
  • Page 11 For manufacturing location, see the label on the instrument. WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the following symbol: Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway.
  • Page 12 Declarations of Conformity...

  • Page 15: Preface

    The heated ion volume, lens stack, and ion optics path in the ISQ 7610 system ensure that the system stays cleaner longer, but when the system no longer meets your analytical needs, restoring performance is quick and easy.

  • Page 16: Related Documentation

    Open the Manuals folder on your desktop.  To open Help • In Xcalibur software, from the ISQ 7610 window in the Method Editor, choose Help > ISQ 7610 Help. • In Chromeleon software, ISQ 7610 Help is available through the Method Editor.

  • Page 17: System Requirements

    Preface System Requirements System Requirements Your ISQ 7610 data system must meet these minimum requirements. System Requirements Hardware • 3.6 GHz dual-core processor enabled • 16 GB RAM with system managed memory enabled • DVD drive • Resolution display 1280 × 1024 (SXGA) •...

  • Page 18: Safety Symbols And Signal Words

    This damage might not be covered under the standard warranty. LIFTING HAZARD: Indicates that a physical injury could or might occur if two or more people do not lift an object. xviii ISQ 7610 User Guide Thermo Scientific...
  • Page 19 RISQUE BIOLOGIQUE : indique qu’un risque biologique va, peut ou pourrait survenir. RISQUE DE BRÛLURE : vous avertit de la présence d’une surface chaude qui peut ou pourrait entraîner des blessures par brûlure. RISQUE D’ÉLECTROCUTION : indique qu’un choc électrique peut ou pourrait survenir. Thermo Scientific ISQ 7610 User Guide...
  • Page 20 CONSULTER LE MANUEL : vous avertit de lire attentivement la documentation de votre instrument afin de garantir votre sécurité et la capacité opérationnelle de l’instrument. Ne pas lire attentivement la documentation peut ou pourrait vous exposer à un risque de blessure physique. ISQ 7610 User Guide Thermo Scientific...

  • Page 21: Hydrogen Safety Precautions

    Before you begin using hydrogen, you should conduct a risk assessment based on the quantity of hydrogen to be used and the conditions of your laboratory. You should ask yourself: “What hydrogen hazards associated with this project are most likely to occur?” Thermo Scientific ISQ 7610 User Guide...
  • Page 22 • There should also be contingency plans in place should an incident occur. • The site emergency response team, as well as the local fire department, should know the location of all hydrogen storage tanks. xxii ISQ 7610 User Guide Thermo Scientific...

  • Page 23: Using Hydrogen With Isq 7610 Mass Spectrometers

    Hydrogen Safety Precautions Using Hydrogen with ISQ 7610 Mass Spectrometers To use hydrogen with the ISQ 7610 instrument, you must always shut off the GC carrier gas before venting or turning off the ISQ 7610 instrument. There are three hydrogen safety screws on the ISQ 7610 instrument that must be in place.
  • Page 24 If a hydrogen cylinder or hydrogen generation system is located within immediate reach, a separate point-of-use shutoff valve is usually not necessary. Line regulators that have their source away from the point of use should have a manual shutoff valve near the point of use. xxiv ISQ 7610 User Guide Thermo Scientific...

  • Page 25: Purchasing Hydrogen

    Properly Storing Hydrogen Storing and handling compressed hydrogen gas and cryogenic liquid hydrogen present potential health and safety hazards. Using proper storage and handling techniques is essential to maintaining a safe work environment. Thermo Scientific ISQ 7610 User Guide...
  • Page 26 • As liquid hydrogen systems also have a cryogenic hazard, additional safety considerations for the use of cryogenic liquids may be necessary. xxvi ISQ 7610 User Guide Thermo Scientific...

  • Page 27: Hydrogen Safety Codes, Standards And References

    • NFPA 55, Standard for the Storage, Use, and Handling of Compressed Gases and Cryogenic Fluids in Portable and Stationary Containers, Cylinders, and Tanks, 2005 Edition • NFPA 68, Standard on Explosion Protection by Deflagration Venting • NFPA 70, National Electrical Code Thermo Scientific ISQ 7610 User Guide xxvii...

  • Page 28: Hazardous Substances Precautions

    AVERTISSEMENT Avant d’utiliser des substances dangereuses (toxiques, nocives, etc.), veuillez lire attentivement les indications et informations relatives au risque reprises sur la fiche de données de sécurité adéquate. Utilisez un équipement de protection individuelle conformément aux exigences de sécurité. xxviii ISQ 7610 User Guide Thermo Scientific...

  • Page 29: Biological Hazard Warning Note

    Consult local environmental and safety regulations for instructions in exhausting fumes from your system. Contacting Us There are several ways to contact Thermo Fisher Scientific for the information you need. Thermo Scientific ISQ 7610 User Guide xxix...
  • Page 30  To get local contact information for sales or service Go to www.unitylabservices.com/en/home.html.  To suggest changes to documentation or to Help Send an e-mail message to the Technical Publications Editor at techpubs-austin@thermofisher.com. ISQ 7610 User Guide Thermo Scientific...

  • Page 31: Confirming Your Gc/Ms System Is Working

    To confirm that the ISQ 7610 system is powered on, make sure the Power light on the front panel is solid green. If it is not lit, the ISQ 7610 system is not powered on. To power it on, reach around the right side of the instrument and pull up the power switch on the back. If the instrument still doesn’t power on, check the electrical connections and wall outlet.

  • Page 32: Verifying The Carrier Gas Flow Rate

    3. If the actual and set point amounts in Col. Flow are the same, then you have good carrier gas flow. If the amounts are different, see the troubleshooting section of your GC user documentation. ISQ 7610 User Guide Thermo Scientific...

  • Page 33: Checking Your Carrier Gas Supply Pressure

    4. Set the second stage regulator pressure between 80 and 100 psi (560 kPa to 700 kPa). Thermo Scientific ISQ 7610 User Guide...

  • Page 34: Checking The Vacuum And Temperature

    Checking the Vacuum and Temperature Checking the Vacuum and Temperature Use the lights on the front of the ISQ 7610 instrument to check the vacuum and temperature of the instrument. To check the vacuum, look at the Vacuum light. When the light is a solid green, the ISQ mass spectrometer is under sufficient vacuum.

  • Page 35: Chapter 2 Changing The Column

    Changing the Column The ISQ 7610 mass spectrometer ships with a factory-tested 15 m x 0.25mm i.d. TG-SQC column, which the Field Service Engineer uses to qualify the instrument. However, once it gets dirty, you cannot purchase a new one, so you need to install a column that accommodates the type and quantity of samples you will be running.

  • Page 36: Replacing The Factory-Installed Column

    7. If your ISQ 7610 MS has a vacuum probe interlock (VPI) and the ExtractaBrite ion source is installed, see “Replacing a column on a ISQ 7610 system with a VPI and an ExtractaBrite ion source” page ISQ 7610 User Guide...

  • Page 37: Replacing A Column In A Isq 7610 System With No Vpi Or An Aei Ion Source

    Replacing a column in a ISQ 7610 system with no VPI or an AEI ion source If your ISQ 7610 system has no VPI or has an AEI ion source installed on the system, follow the instructions below to replace the column.
  • Page 38 1. Lower the oven temperature and allow it to cool. 2. If the ISQ 7610 system is running, shut down and vent it. See the instrument’s hardware manual for instructions. CAUTION BURN HAZARD: The injector, detectors, oven, and transfer line may be hot.
  • Page 39 12. Turn the spring loaded transfer line nut 1 flat (60°) backward so the column is able to move in the ferrule with slight resistance. 13. Line up the outlet of the column with the arrows on the end of the column measuring tool. Thermo Scientific ISQ 7610 User Guide...
  • Page 40 15. Pull the column back from the spring loaded transfer line nut. Do not move the septum from its position on the column. Figure 8. Pulling the Column Back from the Spring Loaded Transfer Line Nut Spring Loaded Column Transfer Line Nut Septum ISQ 7610 User Guide Thermo Scientific...
  • Page 41 20. Loosen the nut by turning it exactly 1 flat (60°) backward. 21. Position the column in the transfer line using the cut septum to measure the correct length you should insert the column. Thermo Scientific ISQ 7610 User Guide...
  • Page 42 26. Close the front door of the GC. 27. Restore working conditions. a. Raise the oven temperature to the initial temperature that you will use. b. Turn on vacuum compensation on the GC. 28. Power on the ISQ 7610 instrument. ISQ 7610 User Guide Thermo Scientific...
  • Page 43 6. Insert the column through the transfer line nut and ferrule, entering through the tapered end of the ferrule. Wipe the column again with a tissue soaked in methanol. Thermo Scientific ISQ 7610 User Guide...
  • Page 44 12. Line up the outlet of the column with the arrows on the end of the column measuring tool. Figure 13. Lining Up the Column in the Column Measuring Tool Column Outlet ISQ 7610 User Guide Thermo Scientific...
  • Page 45 16. Remove the column, transfer line nut and ferrule from the column measuring tool, making sure not to move the septum from its location on the column. 17. Insert the column into the transfer line. Thermo Scientific ISQ 7610 User Guide...
  • Page 46 21. Tighten the nut 1 flat (60°) forward—back to where it is secure enough in the transfer line that you cannot move it. 22. Tighten the nut 1 additional quarter turn. 23. Remove the cut septum. ISQ 7610 User Guide Thermo Scientific...

  • Page 47: Replacing A Column On A Isq 7610 System With A Vpi And An Extractabrite Ion Source

    Power on the ISQ 7610 instrument. Once the ISQ 7610 instrument is pumped own and able to scan, view air water spectra and look for evidence of leaks with a large m/z 28 signal. If you observe a leak, stop scanning and gently tighten the nut in small increments until no leaks appear when scanning.
  • Page 48 On the GC, set the Oven to Off. b. On the ISQ 7610 Dashboard set the MS transfer line temp to 40 °C and the ion source temp to 175 °C (to avoid excessive oxidation of source parts or contamination from the V-lock source plug).
  • Page 49 V-lock source plug is securely placed in the instrument. See Figure IMPORTANT The V-lock source plug should remain attached to the source exchange tool. Do not rotate the source exchange tool handle to attempt to disengage the source plug. Thermo Scientific ISQ 7610 User Guide...
  • Page 50 Note Wear clean, lint- and powder-free gloves when you handle the column and transfer line ferrule. 16. Wipe approximately 300 mm (12 in.) of the column with a tissue soaked in methanol. 17. Choose an appropriate ferrule for the outer diameter of your column. ISQ 7610 User Guide Thermo Scientific...
  • Page 51 23. Use a scoring wafer to score and then remove the last 10 mm of column to provide a clean, well-cut end. 24. Wipe the column with an alcohol soaked-wipe after inserting through the ferrule. Thermo Scientific ISQ 7610 User Guide...
  • Page 52 Tip To avoid collecting dust on the V-lock source plug, store it in a closed container when not in use. 35. Using the source holder, place the ion source on the insertion/removal tool and install the ion source using the vacuum interlock. ISQ 7610 User Guide Thermo Scientific...

  • Page 53: Installing The Column In The Gc Inlet

    Replacing the Factory-Installed Column Once the ISQ 7610 instrument is pumped down and able to scan, view air water spectra and look for evidence of leaks with a large m/z 28 signal. If you observe a leak, stop scanning and gently tighten the nut in small increments until no leaks appear when scanning.
  • Page 54 Check the fitting before looking for the leak elsewhere. CAUTION INSTRUMENT DAMAGE: Do not allow the column flowmeter connector to exceed 80 °C (176 °F). Otherwise, it will melt and damage the instrument. ISQ 7610 User Guide Thermo Scientific...
  • Page 55 (1.3 – 2.0 for a 30 m, 0.25 mm i.d. column). If the column does not report a K-factor within this range or within 0.1 units of the previous stored value, check for a Thermo Scientific ISQ 7610 User Guide...
  • Page 56 Close the GC door. 7. If necessary, condition the column before inserting it into the ISQ 7610 system. Column conditioning consists of passing a carrier gas flow through the column heated to a programmed temperature as described in the column manufacturer’s instructions.
  • Page 57 Hold the column at this temperature for two hours. CAUTION INSTRUMENT DAMAGE: Never exceed the column manufacturer’s maximum operating temperature. ATTENTION DOMMAGES À L’INSTRUMENT : ne dépassez jamais la température de fonctionnement maximum de la colonne indiquée par le fabricant. Thermo Scientific ISQ 7610 User Guide...

  • Page 59: Chapter 3 Tuning The Isq 7610 Mass Spectrometer

    (up to 4 hours) for the instrument components to reach stable vacuum and temperature. If you did not vent the ISQ 7610 instrument (system is hot), it takes approximately 30 minutes for the components to reach vacuum and temperature.

  • Page 60: Accessing Isq 7610 Tuning

     To access ISQ 7610 AutoTune in Xcalibur Software Open the ISQ 7610 Dashboard and click AutoTune Options to open the AutoTune window. Figure 25. Accessing ISQ 7610 AutoTune from the ISQ 7610 Dashboard 1. If you have configured Advanced AutoTune or SmartTune for your EI or AEI ion source you may access those options by right-clicking on AutoTune Options and choosing from a drop-down menu.
  • Page 61 Figure 26. Selecting from AutoTune Options The list of available tune types opens.  To access ISQ 7610 Tuning in Chromeleon Software 1. Go to the ISQ tab on the Chromeleon Console and click Tuning to open the tuning window. See...
  • Page 62 Tuning the ISQ 7610 Mass Spectrometer Accessing ISQ 7610 Tuning Figure 27. Accessing ISQ 7610 Tuning from the Chromeleon Console 2. If you have configured Advanced AutoTune or SmartTune for your EI or AEI ion source you may access those options in the by clicking Advanced AutoTune in the Tuning utility.
  • Page 63 Tuning the ISQ 7610 Mass Spectrometer Accessing ISQ 7610 Tuning Figure 28. Opening the Tuning Panel 3. The AutoTune window with a list of available tune types opens. See Figure 29. Opening the AutoTune Window Thermo Scientific ISQ 7610 User Guide...

  • Page 64: Tune Types

    Tuning the ISQ 7610 Mass Spectrometer Tune Types Tune Types This section explains the different tune types in ISQ 7610 AutoTune. EI Initial Tune EI Full Tune EI Tune EI Check EI Diagnostics Negative CI Tune Positive CI Tune AEI Full Tune...
  • Page 65 Tuning the ISQ 7610 Mass Spectrometer Tune Types Figure 30shows a typical tune report for an EI Full Tune on a system using helium as a carrier gas. Figure 30. Typical EI Full Tune Report Typical results for an EI Full Tune are listed below.

  • Page 66: Ei Tune

    Tuning the ISQ 7610 Mass Spectrometer Tune Types • Multiplier Voltage – Normal Performance: < 2200 V Replace Multiplier: ≥ 2200 V – • Foreline Pressure: < 100 mTorr • Ion Gauge Pressure: < 5 × 10 Torr Note Foreline and ion gauge are optional devices. Their pressures are dependent on column flow rate.

  • Page 67: Ei Diagnostics

    Tuning the ISQ 7610 Mass Spectrometer Tune Types EI Diagnostics EI Diagnostics (default)—Runs a complete set of diagnostics, including a leak check, and generates a report. No tuning is performed. Uses the parameters from the last saved tune. Negative CI Tune CI- Tune (default)—Used to analyze samples with negative CI.
  • Page 68 Figure 31. Typical CI- Tune Report with Methane as a CI Reagent Gas Typical results for a CI- Tune using methane as the reagent gas are listed below. • Peak Intensities: – Base Peak: 452 or 633 – Base Peak ≥ 10,000,000 •...

  • Page 69: Positive Ci Tune

    Tuning the ISQ 7610 Mass Spectrometer Tune Types • Foreline Pressure: < 400 mTorr • Ion Gauge Pressure: < 1 × 10 Torr Note Foreline pressure fluctuates with CI reagent gas flow rate. As the CI reagent gas flow rate increases, the foreline pressure also increases. Ion gauge pressure also increases if an ion gauge is installed on the system.
  • Page 70 Tuning the ISQ 7610 Mass Spectrometer Tune Types Figure 32. Typical C+- Tune Report with Methane as a CI Reagent Gas Tip If you intend to use ammonia reagent gas, attach methane to one CI reagent gas port and ammonia to the other port. Tune the instrument using methane, then switch to the ammonia port.

  • Page 71: Aei Full Tune

    Tuning the ISQ 7610 Mass Spectrometer Tune Types Note Emission current is an input value, and it should match the value set in the tune. • Multiplier Voltage – Normal Performance: < 2200 V at 3 × 10 detector gain –...

  • Page 72: Aei Check

    Tuning the ISQ 7610 Mass Spectrometer  To tune the ISQ 7610 Mass Spectrometer using AutoTune in Xcalibur 1. Select the tune type you want to use from the list of available tune types. See Figure Figure 33.
  • Page 73 Tuning the ISQ 7610 Mass Spectrometer Tuning the ISQ 7610 Mass Spectrometer Figure 34. Displaying a Tune Report 3. Select the Show Spectra checkbox to show the spectra while the system is tuning. 4. Click the Start button to begin tuning.
  • Page 74 5. Once the tune completes, your tune report will open in the ISQ Series Tune Results Viewer. If you did not select the Display Report When Complete checkbox, you can click View Tune Report on the ISQ 7610 Dashboard and view the report. ISQ 7610 User Guide...
  • Page 75 Tuning the ISQ 7610 Mass Spectrometer Tuning the ISQ 7610 Mass Spectrometer Figure 36. Viewing a Tune Report. Note The Error Action is not diagnostic. It indicates what happens during the tune if a specific device cannot meet the tuning criteria. Any Stop comments on error actions do not mean the tune has failed.
  • Page 76 Tuning the ISQ 7610 Mass Spectrometer Tuning the ISQ 7610 Mass Spectrometer 6. Compare this tune with a previous tune report. Some changes in peak height are normal, but if the difference is significant, see Troubleshooting. If you have recently serviced the instrument, you most likely have a leak at the column, vent valve or near the component you just serviced.

  • Page 77: Using Smarttune

    10. If the sensitivity and resolution are adequate for running the initial samples, you are ready to develop or run a method. Using SmartTune If you configured the ISQ 7610 software to use the EI SmartTune or AEI SmartTune application, follow the instructions below to use the application. Thermo Scientific...
  • Page 78 Using SmartTune  To use the SmartTune application 1. Right-click AutoTune Options on the ISQ 7610 Dashboard and choose AEI SmartTune or EI SmartTune depending on which ion source is installed on your instrument. See Figure Figure 40. Opening the SmartTune Application 2.
  • Page 79 Tuning the ISQ 7610 Mass Spectrometer Using SmartTune Figure 41. Viewing the SmartTune Application 3. If this is the first tune after installing a clean ion source, check the box indicating that. Click the green arrow on the right side of the panel to start the SmartTune application.
  • Page 80 7. See Figure 44 below for the SmartTune workflow. Figure 44. SmartTune Workflow  To Tune the ISQ 7610 MS Using Chromeleon Software 1. Choose a tune from AutoTune window. Click Start. See Figure ISQ 7610 User Guide Thermo Scientific...
  • Page 81 2. View the tuning status in the audit trail under the ISQ tab on the Chromeleon Console. 3. To open a tune report, go to the Available Tune Results on the Tuning panel. See Figure Figure 46. Locating ISQ 7610 Tune Reports in Chromeleon Software Thermo Scientific ISQ 7610 User Guide...
  • Page 82 Tuning the ISQ 7610 Mass Spectrometer Using SmartTune  To use the SmartTune application in Chromeleon Software Note The instrument configuration settings determine whether or not Advanced AutoTune and/or the EI or AEI SmartTune options are shown on this page. If they are missing, please check the instrument configuration.

  • Page 83: Tuning For Dftpp And Bfb

     To tune the instrument for analyzing DFTPP or BFB using custom tune types 1. Open the ISQ 7610 Dashboard and click AutoTune Options to open the AutoTune window. See Figure...
  • Page 84 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB Figure 49. Accessing ISQ 7610 AutoTune from the ISQ 7610 Dashboard 2. Select Advanced AutoTune from the drop-down menu. See Figure ISQ 7610 User Guide Thermo Scientific...
  • Page 85 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB Figure 50. Selecting from AutoTune Options 1. Click Tune Types to create a new tune type. See Figure Figure 51. Creating a New Tune Type 2. In the Tune Types dialog box, select a tune type to edit and click the Copy button. See...
  • Page 86 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB Figure 52. Using the Tune Types Dialog Box 3. The Edit Tune Type window opens. In the Name field, name the new tune type DFTPP Tune or BFB Tune depending on your requirement. Change the description if you prefer.
  • Page 87 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB Figure 54. Tuning Target Ion Ratios 5. For DFTPP—Enter the values below for the target ion ratios. See Table Table 1. Target Ion Ratios for DFTPP Target Mass Ratio (%) (amu) 6.
  • Page 88 Figure 55. DFTPP Tune Targets 7. Save as DFTPP Tune. 8. For BFB—Enter the values below for the target ion ratios. See Table Table 2. Target Ion Ratios for BFB Target Mass Ratio (%) (amu) 9. See Figure 56 for an example of a correct BFB Tune.
  • Page 89 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB Figure 56. BFB Tune Targets 10. Save as BFB Tune. 11. Tune the instrument with the newly created tune types as described in Tuning the ISQ 7610 Mass Spectrometer.
  • Page 90 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB Figure 57. Locating Air & Water/Tune on the ISQ 7610 Dashboard 2. The ISQ Manual Tune utility opens. The last saved tune opens. If your DFTPP or BFB Tune was not the last saved tune, select Load Tune File, and open the correct tune file.
  • Page 91 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB The example in Figure 59 shows a DFTPP run that had a low m/z 198. In this example, the m/z 198 intensity was low, and the tune failed. To increase the m/z 198 intensity, the high mass intensity was lowered in the target tune factors.
  • Page 92 Tuning the ISQ 7610 Mass Spectrometer Tuning for DFTPP and BFB 4. When the DFTPP tune is run after the 502 amu target mass was lowered, the m/z 198 intensity increases, and the tune passes. See Figure Figure 61. DFTPP Tune Results with High m/z 198 5.

  • Page 93: Chapter 4 Creating A Method

    Creating a GC-MS Method in Chromeleon Software Accessing the Method Editor To create a method for the ISQ 7610 mass spectrometer, the TRACE 1600 or 1610 GC, and your autosampler, use the Method Editor. The Method Editor is accessed differently on systems running Xcalibur software and those running Chromeleon software.
  • Page 94 Creating a Method Accessing the Method Editor Figure 62. Accessing the Method Editor from the ISQ 7610 Dashboard 2. The Method Editor window opens. See Figure 63. Icons of all connected instruments appear in the left pane. You may only configure one instrument at a time. The bright green arrow on the lower right corner of the instrument icon indicates the active instrument.
  • Page 95  To access the Method Editor using Chromeleon software To access the Method Editor for the ISQ 7610 mass spectrometer, the TRACE 1600 or 1610 GC, and your autosampler, go to Start > Thermo Chromeleon > Chromeleon 7 and open the Chromeleon software application The Chromeleon Console opens to the Thermo Scientific GCMS Home Page.

  • Page 96: Creating A Gc-Ms Method In Xcalibur Software

    Xcalibur software Note For information about creating a method for specific instruments and software, refer to the appropriate documentation. 1. Click Method Editor on the ISQ 7610 Dashboard to open the Method Editor. See Figure ISQ 7610 User Guide Thermo Scientific...

  • Page 97: Creating An Autosampler Method In Xcalibur Software

    Creating a Method Creating a GC-MS Method in Xcalibur Software Figure 65. Accessing the Method Editor from the ISQ 7610 Dashboard Method Editor Creating an Autosampler Method in Xcalibur Software Use the method editor to create a method for the autosampler if you have one.
  • Page 98 (Optional) Cooled/Heated Trays—Enter ID information for your primary and, if used, secondary trays. 4. Click Create New Method. 5. Use these instructions to configure the GC Liquids general method settings (see Figure 67): ISQ 7610 User Guide Thermo Scientific...
  • Page 99 With a viscous sample, the syringe is filled more slowly than if it was non-viscous. Since the amount of time saved is so small, setting this option to Yes might be easier. Thermo Scientific ISQ 7610 User Guide...
  • Page 100 A, B, C, or D or with solvents A and B or C and D. Typically, there is 0 or 1 cycles of pre-injection purges. ISQ 7610 User Guide Thermo Scientific...

  • Page 101: Creating A Method For The Isq 7610 Mass Spectrometer

    B or C and D. Typically, there are 1 to 5 cycles of post-injection purges. Creating a Method for the ISQ 7610 Mass Spectrometer Click the ISQ 7610 icon in the left pane to create a method for the ISQ 7610 mass spectrometer.
  • Page 102 5. Select EI from the Ionization Mode pull-down menu. Note Only use CI if you have installed a CI ion volume in the ISQ 7610 instrument and you have connected CI reagent gas to your system. If you have CI, select a CI Gas Type from the pull-down menu and set the CI Gas Flow.
  • Page 103 The Time (min) column is used to set the time that the ISQ 7610 system begins to acquire data after the GC starts. It is typical to have enough of a time delay to allow the solvent to get through the column before starting an acquisition.
  • Page 104 All of these scans use the last tune file that was saved to the instrument. b. The Mass List or Range column tells the ISQ 7610 system what masses it needs to scan. In full-scan mode, enter the start and end mass separated by a dash. In SIM mode, enter individual masses or multiple values in this field (as long as they are separated by a comma).
  • Page 105 Note If some of the columns mentioned below are not shown in the Groups pane, you can right-click on a heading and display them. You can also reorganize the columns by clicking on the heading of a column and dragging it to the left or right. Thermo Scientific ISQ 7610 User Guide...
  • Page 106 The Use Last Tuned Detector Gain column indicates that you want to use the detector gain set in ISQ 7610 AutoTune or set and scanned in manual tune. If you do not need to use the gain set in ISQ 7610 AutoTune, then you can set the gain manually.
  • Page 107 Gain option to indicate that you want to use the detector gain set in ISQ 7610 AutoTune. If you do not need to use the gain set in ISQ 7610 AutoTune, then you can set the gain manually. Higher gains give larger signals, but may shorten the lifetime of the detector or saturate the electrometer with too much signal when concentrated samples are detected.
  • Page 108 For complex SIM methods, this option will help ensure a method is created that can achieve the requested scans per peak. ISQ 7610 User Guide Thermo Scientific...
  • Page 109 Set the minimum window to the lowest safe value to prevent compound retention times from drifting outside the acquisition window. Thermo Scientific ISQ 7610 User Guide...
  • Page 110 If you want to link to an external method, select the Link to External File check box. You may link to a .csv or .xml method file. Note In order to edit the scans within the ISQ 7610 Method Editor, clear the Link to External File check box.
  • Page 111 Click Open to open the method in SIMBridge. If necessary, change the method headings in your original file to match those in the method editor. A green check mark appears when your method is validated. Thermo Scientific ISQ 7610 User Guide...
  • Page 112 In the RT column, enter retention times for SIM methods. The retention time is the time it takes an analyte to pass from the column inlet to the detector. ISQ 7610 User Guide Thermo Scientific...
  • Page 113 You may set the number of adjacent analytes to show in your method by using the Number of Adjacent Analytes to Show selection box found in the Options dialog box accessed by the ISQ 7610 main menu. See Figure 78.
  • Page 114 Also, if your list contains duplicate compounds, the middle of one of the duplicate peak bars will show an orange crossed pattern, instead of the usual white. Delete one of the duplicate compounds to avoid problems with data analysis. ISQ 7610 User Guide Thermo Scientific...
  • Page 115 Figure 81. Importing Methods 12. Choose ISQ 7610 | Import Timed Scans to import .csv or .xml files of previous methods. The software will only load files in valid formats. If your file is not valid you will receive an error message and will not be able to import the file into the Method Editor.
  • Page 116 Method Editor. 14. Choose ISQ 7610 | Export Timed Scans to export your method as a .csv file. If you prefer editing your methods in spreadsheet applications, you may want to use this option.
  • Page 117 The confirming ion now appears in the list as a target ion. New Target Ion d. To add a new confirming ion to a target ion in the list, select the confirming ion of interest. Thermo Scientific ISQ 7610 User Guide...
  • Page 118 Creating a GC-MS Method in Xcalibur Software Confirming Ion e. Drag the confirming ion under the target ion of interest. Target Ion The confirming ion appears in the list under the selected quantitation ion. ISQ 7610 User Guide Thermo Scientific...

  • Page 119: Creating A Method For The Gc In Xcalibur Software

    16. Choose ISQ 7610 | Create Segment List From Timed Scan List to import a general acquisition method. 17. Choose ISQ 7610 | View Tune Report to view the latest tune report the method will use. Choose Report Options to open the Report Options dialog box (see...
  • Page 120 As an example, with the default value of 10 minutes, if the GC is ready to receive an injection, but does not receive it after ten minutes, the GC ISQ 7610 User Guide Thermo Scientific...
  • Page 121 This is not typically used because the material that is purged from the column in this step would go into the ISQ 7610 mass spectrometer, which can lead to contamination. If you want to use this feature, set the GC oven temperature, as well as the amount of time to remain at that temperature after the analytical run is complete.
  • Page 122 The default purge flow value is 5 mL/min. This reduces the buildup of contaminants in the injector, on the column, and in the ISQ 7610 instrument. If you perform a splitless injection, even if the split flow is set, the split flow will be turned off for the splitless time.
  • Page 123 20 mL/min because contaminants can build up in the injector, column, and ISQ 7610 instrument, which can affect the system performance. It is also possible for air to diffuse back into the column with low split flows when the column head pressure is low.
  • Page 124 Add the inlet or detector to the current instrument configuration. Go to Start | All Programs | Thermo Foundation 3.0 | Instrument Configuration. c. In the Configured Devices panel, click the TRACE 1600 or TRACE 1610 GC icon. Select Configure. ISQ 7610 User Guide Thermo Scientific...
  • Page 125 Click Get. The software automatically detects the attached modules. Select the gases as appropriate to your setup. The default Channel names are Channel 1 and Channel 2. You may want to change them to something more descriptive. Your hardware is now configured. Thermo Scientific ISQ 7610 User Guide...

  • Page 126: Creating A Gc-Ms Method In Chromeleon Software

    Creating a GC-MS Method in Chromeleon Software If your system is running Chromeleon software, follow the instructions in this section to create a method for the autosampler, GC, and ISQ 7610 mass spectrometer.  To create a GC-MS method in Chromeleon Software 1.
  • Page 127 System follow. Refer to your autosampler documentation for more detailed information about settings. 2. Go to Create > Instrument Method. Figure 90. Creating an Instrument Method 3. The Instrument Method Wizard – Select Instrument window opens. See Figure Thermo Scientific ISQ 7610 User Guide...
  • Page 128 5. The Instrument Method Wizard – System: General Settings window opens. See Figure Figure 92. Instrument Method Wizard – System: General Settings 6. You can set the GC Run Time for the method here, or load it with the other GC settings later. Click Next. ISQ 7610 User Guide Thermo Scientific...
  • Page 129 (Optional) Cooled/Heated Trays—Enter ID information for your primary and, if used, secondary trays. 10. Click Create New Method. 11. Use the following instructions to configure the GC Liquids general method settings. See Figure Thermo Scientific ISQ 7610 User Guide...
  • Page 130 With a viscous sample, the syringe is filled more slowly than if it was non-viscous. Since the amount of time saved is so small, setting this option to Yes might be easier. ISQ 7610 User Guide Thermo Scientific...
  • Page 131 Always include some sample rinses, either before or after injection, to make sure you do not have sample carryover from one injection to the next. Configure the settings so that the syringe is Thermo Scientific ISQ 7610 User Guide...
  • Page 132 If you use constant flow, the carrier gas pressure will increase as the column temperature increases to keep the flow constant. Constant flow is more common. Typical flow rates are 1-3 mL/min. The pressure depends on the column ISQ 7610 User Guide Thermo Scientific...
  • Page 133 The reverse is true also. c. For best results, check the Constant Septum Purge box to use the septum purge, which means additional carrier gas will go through the injector. The default purge Thermo Scientific ISQ 7610 User Guide...
  • Page 134 GC will not reach the initial temperature. If you set the temperature above the limit, the GC column will be damaged. You can set the initial hold time to a value between 0 and 999.99 minutes. The typical initial temperature is at least 10 °C (18 °F) ISQ 7610 User Guide Thermo Scientific...
  • Page 135 GC configuration. 24. To add an inlet or detector to the Method editor user interface: a. Attach the inlet or detector to the GC. Refer to the GC documentation for instructions. Thermo Scientific ISQ 7610 User Guide...
  • Page 136 Acquisition-General, Acquisition-Timed, or Tune Mode. An Acquisition method is used to collect data. Note The maintenance method is used to bake out or cool down the ISQ 7610 instrument during a sequence. This can be useful if you know you want to perform these tasks and you want to do them in an automated way as part of your data acquisition.
  • Page 137 31. Select EI from the Ionization Mode pull-down menu. Note Only use CI if you have installed a CI ion volume in the ISQ 7610 instrument and you have connected CI reagent gas to your system. If you have CI, select a CI Gas Type from the pull-down menu and set the CI Gas Flow.
  • Page 138 The Time (min) column is used to set the time that the ISQ 7610 system begins to acquire data after the GC starts. It is typical to have enough of a time delay to allow the solvent to get through the column before starting an acquisition.
  • Page 139 In SIM mode, the Dwell or Scan Times column defines the amount of time (in seconds) that the ISQ 7610 instrument will look at your SIM ion mass. If you are in Full-Scan mode, the Dwell or Scan Times column determines the amount of time for each individual scan.
  • Page 140 Note If some of the columns mentioned below are not shown in the Groups pane, you can right-click on a heading and display them. You can also reorganize the columns by clicking on the heading of a column and dragging it to the left or right. ISQ 7610 User Guide Thermo Scientific...
  • Page 141 The Use Last Tuned Detector Gain column indicates that you want to use the detector gain set in ISQ 7610 AutoTune or set and scanned in manual tune. If you do not need to use the gain set in ISQ 7610 AutoTune, then you can set the gain manually.
  • Page 142 Gain option to indicate that you want to use the detector gain set in ISQ 7610 AutoTune. If you do not need to use the gain set in ISQ 7610 AutoTune, then you can set the gain manually. Higher gains give larger signals, but may shorten the lifetime of the detector or saturate the electrometer with too much signal when concentrated samples are detected.
  • Page 143 For complex SIM methods, this option will help ensure a method is created that can achieve the requested scans per peak. Thermo Scientific ISQ 7610 User Guide...
  • Page 144 Set the minimum window to the lowest safe value to prevent compound retention times from drifting outside the acquisition window. ISQ 7610 User Guide Thermo Scientific...
  • Page 145 If you want to link to an external method, select the Link to External File check box. You may link to a .csv or .xml method file. Note In order to edit the scans within the ISQ 7610 Method Editor, clear the Link to External File check box.
  • Page 146 Click Open to open the method in SIMBridge. If necessary, change the method headings in your original file to match those in the method editor. A green check mark appears when your method is validated. ISQ 7610 User Guide Thermo Scientific...
  • Page 147 You may also right-click this window to search for an analyte within your method. This function is useful if you need to edit an analyte in a complex method. Thermo Scientific ISQ 7610 User Guide...
  • Page 148 You may set the number of adjacent analytes to show in your method by using the Number of Adjacent Analytes to Show selection box found in the Options dialog box accessed by the ISQ 7610 main menu. See Figure 78.
  • Page 149 Delete one of the duplicate compounds to avoid problems with data analysis. Figure 110. Validating a Method 37. To import another MS method, choose Import. See Figure Thermo Scientific ISQ 7610 User Guide...
  • Page 150 41. Choose Import | Create Compound Data Store Export File to prepare your file for the compound data store in the TraceFinder application. a. To create a new target ion from the list of confirming ions with identical retention times for a compound, select the confirming ion of interest. ISQ 7610 User Guide Thermo Scientific...
  • Page 151 Creating a GC-MS Method in Chromeleon Software Compound Name Confirming b. Drag the confirming ion to the compound name at the top of the list. Compound Name c. The confirming ion now appears in the list as a target ion. Thermo Scientific ISQ 7610 User Guide...
  • Page 152 New Target Ion d. To add a new confirming ion to a target ion in the list, select the confirming ion of interest. Confirming Ion e. Drag the confirming ion under the target ion of interest. ISQ 7610 User Guide Thermo Scientific...
  • Page 153 43. Choose Import | View Tune Report to view the latest tune report the method will use. Choose Report Options to open the Report Options dialog box (see Figure 82) and add identifying information to the tune report. Thermo Scientific ISQ 7610 User Guide...
  • Page 154 From the Method Type drop-down box, select Tune Mode. See Figure 113. Figure 113. Selecting a Tune Mode Method b. Under Tune Template set the following parameters: Check View Tune Data to view the tune data. ISQ 7610 User Guide Thermo Scientific...
  • Page 155 46. Enter any comments or notes for your method and click Finish. 47. The New Instrument Method (Instrument Method) Chromeleon Chromatography Studio window opens. Click the Save icon to save your method. See Figure Thermo Scientific ISQ 7610 User Guide...
  • Page 156 49. Enter your method in the Object Name box and click Save. Your method is now saved. 50. You can view all instrument methods on your system by opening the Chromeleon Console and selecting Data from the left menu. See Figure 117. ISQ 7610 User Guide Thermo Scientific...
  • Page 157 51. To edit a method, select it from the list. The method opens in the Chromatography Studio. See Figure 118. Figure 118. Editing a Method 52. Select a device from the left menu and edit its parameters as described in this chapter. Thermo Scientific ISQ 7610 User Guide...

  • Page 159: Chapter 5 Using Autosim

    This chapter will help you use the AutoSIM software utility to set up and run a SIM Ion Study. As well as instructions for setting up and running each study, this chapter gives you the steps for importing the resulting list of SIM ions into the ISQ 7610 method editor and accessing them for routine use.
  • Page 160 Figure 119. Accessing AutoSIM on the ISQ Dashboard AutoSIM 2. Click the Create a New Study icon on the left to create a new study. Figure 120. New AutoSIM Study Create a New Study Icon 3. A new study window opens. ISQ 7610 User Guide Thermo Scientific...
  • Page 161 5. Select an instrument method file and click Open. 6. You may set the Mass Range, Start Time, and Stop Time. See Figure 122. Any changes you make to your MS method here will override the method editor settings. Thermo Scientific ISQ 7610 User Guide...
  • Page 162 Tip Create a folder for all files associated with your AutoSIM study. Otherwise, the study results files will be saved in the general instrument method folder and crowd it. See Figure 123. Figure 123. Creating an AutoSIM Study Folder ISQ 7610 User Guide Thermo Scientific...
  • Page 163 Figure 125. Acquiring Acquisition Data Acquire Data Icon New Study Name 14. (CI Only) If you are running a chemical ionization (CI) study, select Positive or Negative from the Ion Polarity pull-down menu. See Figure 126. Thermo Scientific ISQ 7610 User Guide...
  • Page 164 Figure 127. Application Settings and SIM Ion Study Settings Application SIM Ion Study Settings Settings 16. By default, SIM ions are sorted by highest intensity. In the SIM Ion Study Settings box, you may select SIM ions according to the following criteria. ISQ 7610 User Guide Thermo Scientific...
  • Page 165 17. Click the Acquire Data icon to run your samples. See Figure 125. Note AutoSIM calculates the number of injections needed based on the compound list and vial positions you assigned. 18. The Submit Study for Acquisition window opens. See Figure 128. Thermo Scientific ISQ 7610 User Guide...
  • Page 166 The results displayed correspond to the peak topped by the green triangle. The Mass by Highest Intensity pane contains a list the highest intensity ions at the indicated retention time. Note Background subtraction updates the ions in the Mass by Highest Intensity pane. ISQ 7610 User Guide Thermo Scientific...
  • Page 167 21. Select the check box next to the SIM ions you want to send to the working list. 22. Click the green arrow icon to push the SIM ions you selected to the working list. See Figure 130. Thermo Scientific ISQ 7610 User Guide...
  • Page 168 24. Once you have selected all your SIM ions, go to File | Save As and export your SIM ion study as a .csv file. See Figure 131. Figure 131. Exporting your SIM Ion Study ISQ 7610 User Guide Thermo Scientific...

  • Page 169: Importing Transitions To The Method Editor

    3. If you have the desired method open in AutoSIM, click the Link to External File in the ISQ 7610 method editor to open your method in the ISQ 7610 method editor a. After clicking Link to External File, the SIMBridge dialog box opens. Choose the language of your method file from the Source Locale drop-down menu.
  • Page 170 Click Open to open the method in SIMBridge. d. If necessary, change the method headings in your original file to match those in the method editor. A green check mark appears when your method is validated. ISQ 7610 User Guide Thermo Scientific...
  • Page 171 Click Open and the external method will be opened in the method editor. 4. Click the ISQ 7610 icon in the method editor side pane. 5. Select Acquisition-Timed from the Method Type drop-down menu. 6. From the top menu, select ISQ 7610 | Import Timed Scans. See Figure 136.

  • Page 172: Determining Sim Ions In Chromeleon

    7. Browse to the location where you saved the .csv file you created in AutoSIM. The software informs you if your .csv file is valid. See Figure 137. Figure 137. Linking to an External .csv File 8. Click Show Analysis and review the imported list of compounds. 9.
  • Page 173 AutoSIM. Enter your Chromeleon User Name and Password to continue. 2. Click the Create a New Study icon on the left to create a new study. Figure 139. New AutoSIM Study Create a New Study Icon 3. A new study window opens. Thermo Scientific ISQ 7610 User Guide...
  • Page 174 5. If you are importing a file created on another system, click the import icon. Otherwise, select an instrument method file and click Open. 6. The SIMBridge dialog box opens. Choose the language of your method file from the Source Locale drop-down menu. See Figure 141. ISQ 7610 User Guide Thermo Scientific...
  • Page 175 10. Your method opens in AutoSIM. AutoSIM uses the GC and autosampler parameters from this method file. You may set the Mass Range, Start Time, and Stop Time. See Figure 122. Any changes you make to your MS method here will override the method editor settings. Thermo Scientific ISQ 7610 User Guide...
  • Page 176 Tip Create a folder for all files associated with your AutoSIM study. Otherwise, the study results files will be saved in the general instrument method folder and crowd it. See Figure 123. Figure 144. Creating an AutoSIM Study Folder ISQ 7610 User Guide Thermo Scientific...
  • Page 177 Figure 146. Acquiring Acquisition Data Acquire Data Icon New Study Name 18. (CI Only) If you are running a chemical ionization (CI) study, select Positive or Negative from the Ion Polarity pull-down menu. See Figure 126. Thermo Scientific ISQ 7610 User Guide...
  • Page 178 Figure 148. Application Settings and SIM Ion Study Settings Application SIM Ion Study Settings Settings 20. By default, SIM ions are sorted by highest intensity. In the SIM Ion Study Settings box, you may select SIM ions according to the following criteria. ISQ 7610 User Guide Thermo Scientific...
  • Page 179 21. Click the Acquire Data icon to run your samples. See Figure 125. Note AutoSIM calculates the number of injections needed based on the compound list and vial positions you assigned. 22. The Submit Study for Acquisition window opens. See Figure 128. Thermo Scientific ISQ 7610 User Guide...
  • Page 180 24. The Acquisition Status pane displays status information about the running instrument. If the pane is not currently visible, click the Acquisition Status button. See Figure 150. Figure 150. Displaying the Acquisition Status Pane Acquisition Status Button ISQ 7610 User Guide Thermo Scientific...
  • Page 181 The results displayed correspond to the peak topped by the green triangle. The Mass by Highest Intensity pane contains a list the highest intensity ions at the indicated retention time. Note Background subtraction updates the ions in the Mass by Highest Intensity pane. Thermo Scientific ISQ 7610 User Guide...
  • Page 182 28. Select the check box next to the SIM ions you want to send to the working list. 29. Click the green arrow icon to push the SIM ions you selected to the working list. See Figure 130. ISQ 7610 User Guide Thermo Scientific...
  • Page 183 31. Once you have selected all your SIM ions, go to File | Save As and export your SIM ion study as a .csv file. See Figure 131. Figure 155. Exporting your SIM Ion Study Thermo Scientific ISQ 7610 User Guide...

  • Page 184: Importing Transitions To The Chromeleon Instrument Method Editor

    Tip Click the link in the Instrument Method box in AutoSIM to open Chromeleon. 2. Select the Acquisition – Timed method type. Figure 156. Opening Instrument Method in Xcalibur 3. In the MS Settings pane, select ISQ 7610 > Import Timed Scans. Figure 157. Importing Timed Scans ISQ 7610 User Guide...
  • Page 185 6. Click Open to open the method in SIMBridge. 7. If necessary, change the method headings in your original file to match those in the method editor. A green check mark appears when your method is validated. Thermo Scientific ISQ 7610 User Guide...
  • Page 186 8. Click Open and the external method will be opened in the Chromeleon Instrument Method editor. 9. Click the ISQ 7610 icon in the method editor side pane. 10. Click Show Analysis and review the imported list of compounds. Figure 161. Selecting Show Analysis Show Analysis 11.

  • Page 187: Chapter 6 Running A Sample

    Once your sample is prepared, you can transfer it to a sealed vial so that you can inject it into the GC. It is important to use a sealed vial because you do not want the concentration to be altered, which is what happens when the solvent evaporates. Thermo Scientific ISQ 7610 User Guide...

  • Page 188: Running A Sequence In Xcalibur Software

    To run a new sequence of samples 1. Double-click the Xcalibur software icon on your computer desktop. 2. In the Xcalibur Roadmap window, click the Sequence Setup icon. Figure 162. Sequence Setup in Xcalibur Sequence Setup ISQ 7610 User Guide Thermo Scientific...
  • Page 189 You can also right-click on the field to clear, copy, or paste into the field, as well as add and delete rows. Note If you do not have some of the columns discussed here, you can add them by selecting Change | Column Arrangement in the main menu. Thermo Scientific ISQ 7610 User Guide...
  • Page 190 Note Your file name cannot contain spaces. Figure 164. Naming a File Tip You can double-click in the File Name field and browse to a raw file on your computer. ISQ 7610 User Guide Thermo Scientific...
  • Page 191 You can also right-click on the field to clear, copy, or paste into the field, browse to a folder on your computer, or add and delete rows. Figure 165. Selecting a Folder to Store Data Files Thermo Scientific ISQ 7610 User Guide...
  • Page 192 9. Click the first row in the Position column and enter the sample’s vial number if you are using an autosampler. You can also right-click on the field to clear, copy, or paste into the field, as well as add and delete rows. Figure 167. Entering the Sample Vial Position ISQ 7610 User Guide Thermo Scientific...
  • Page 193 12. Now that you’ve set up one row of your sequence, you can stop or you can continue adding tasks. Click in the next row and repeat steps 2-10 for each additional task in your sequence. Remember, you can right-click in most fields to add more rows as you go. Thermo Scientific ISQ 7610 User Guide...
  • Page 194 13. Once you’ve created a sequence of all your samples, select File | Save or click the icon to save the sequence. 14. Enter a comment and click OK. Figure 170. Entering a Comment About Your Sequence 15. Enter the name of your sequence and click the Save button. ISQ 7610 User Guide Thermo Scientific...
  • Page 195 Running a Sample Running a Sequence in Xcalibur Software Figure 171. Naming and Saving your Sequence Thermo Scientific ISQ 7610 User Guide...
  • Page 196 To run the whole sequence, select Actions | Run Sequence from the main menu. You can also just click the icon on the main tool bar. 17. In the Run Sequence window, customize the way you want your sequence to run. ISQ 7610 User Guide Thermo Scientific...
  • Page 197 In the Programs group, you can browse to the executables for Pre-Acquisition and Post-Acquisition. These fields are used to automatically run processing methods after the data acquisition is complete. Because you are developing your method, you haven’t defined a processing method for this field. Thermo Scientific ISQ 7610 User Guide...
  • Page 198 • Standby—Select this option to leave the instrument fully ready after an acquisition. • Off—Select this option to turn off the ISQ 7610 mass spectrometer by turning off the heaters, turbo pump and foreline pump. IMPORTANT If you are using hydrogen as a carrier gas, manually turn off the GC after the sequence completes if you are using this setting.
  • Page 199 19. In the Save As window, select a location on your computer that you want to store your sequence. Then click the Save button. 20. Once your sequence completes, you are ready to explore your data. Thermo Scientific ISQ 7610 User Guide...

  • Page 200: Running A Sequence In Chromeleon Software

    Running a Sequence in Chromeleon Software Once you’ve prepared your sample, you can use Chromeleon software to run your sample(s). Chromeleon uses a sequence to prepare the instruments for data acquisition, as well as monitor the injection and collection of data from the GC/MS. A sequence can be used to acquire data from a sample or prepare the system for maintenance.
  • Page 201 4. Click Next through the wizard and then click Finish. Name and save the sequence. Figure 176. Naming and Saving a Sequence 5. The sequence opens in the Chromeleon Console. Change the injection volume and vial position if needed and click Start. Thermo Scientific ISQ 7610 User Guide...
  • Page 202 Running a Sample Running a Sequence in Chromeleon Software Figure 177. Selecting a Folder to Store Data Files ISQ 7610 User Guide Thermo Scientific...
  • Page 203 8. Click the first row in the Position column and enter the sample’s vial number if you are using an autosampler. You can also right-click on the field to clear, copy, or paste into the field, as well as add and delete rows. Figure 179. Entering the Sample Vial Position Thermo Scientific ISQ 7610 User Guide...
  • Page 204 11. Now that you’ve set up one row of your sequence, you can stop or you can continue adding tasks. Click in the next row and repeat steps 2-10 for each additional task in your sequence. Remember, you can right-click in most fields to add more rows as you go. ISQ 7610 User Guide Thermo Scientific...
  • Page 205 12. Once you’ve created a sequence of all your samples, select File | Save or click the icon to save the sequence. 13. Enter a comment and click OK. Figure 182. Entering a Comment About Your Sequence 14. Enter the name of your sequence and click the Save button. Thermo Scientific ISQ 7610 User Guide...
  • Page 206 Running a Sample Running a Sequence in Chromeleon Software Figure 183. Naming and Saving your Sequence ISQ 7610 User Guide Thermo Scientific...
  • Page 207 To run the whole sequence, select Actions | Run Sequence from the main menu. You can also just click the icon on the main tool bar. 16. In the Run Sequence window, customize the way you want your sequence to run. Thermo Scientific ISQ 7610 User Guide...
  • Page 208 In the Programs group, you can browse to the executables for Pre-Acquisition and Post-Acquisition. These fields are used to automatically run processing methods after the data acquisition is complete. Because you are developing your method, you haven’t defined a processing method for this field. ISQ 7610 User Guide Thermo Scientific...
  • Page 209 • Standby—Select this option to leave the instrument fully ready after an acquisition. • Off—Select this option to turn off the ISQ 7610 mass spectrometer by turning off the heaters, turbo pump and foreline pump. IMPORTANT If you are using hydrogen as a carrier gas, manually turn off the GC after the sequence completes if you are using this setting.
  • Page 210 18. In the Save As window, select a location on your computer that you want to store your sequence. Then click the Save button. Once your sequence completes, you are ready to explore your data. ISQ 7610 User Guide Thermo Scientific...

  • Page 211: Exploring Your Data

     To view your data in FreeStyle: 1. Double-click the Xcalibur software icon on your computer desktop. 2. In the Xcalibur Roadmap window, click the FreeStyle icon. Figure 186. Xcalibur Roadmap FreeStyle Thermo Scientific ISQ 7610 User Guide...
  • Page 212 7. Select Trace Type > Mass Range and enter the mass range for your compound. For OFN, which has a dominant ion at m/z 272, select a mass range that contains only this ion. By ISQ 7610 User Guide Thermo Scientific...
  • Page 213 Chromatogram Ranges pane and manually enter the time range to display. The full scan shows the ions from OFN (at m/z 222, 241, and 272) much more clearly than without the background subtraction. Thermo Scientific ISQ 7610 User Guide...
  • Page 214 Without background subtraction, the full scan would look like the graphic below. Figure 192. OFN in Full Scan Without Background Subtraction 8. There are many other ways to change the way you data displays in FreeStyle software. See the FreeStyle software online help for details. ISQ 7610 User Guide Thermo Scientific...

  • Page 215: Optimizing Your Method

    If the GC oven is not at the boiling point, the compounds will not volatilize and they will become immobilized. Changing ramp rates is usually used to separate coeluting peaks. Thermo Scientific ISQ 7610 User Guide...

  • Page 216: Finding The Best Way To Make An Injection

    A narrower mass range will also allow you to decrease the scan rate and get the same chromatographic peak sampling. Breaking your MS method into groups allows you to create compound-specific MS settings to optimize your data. ISQ 7610 User Guide Thermo Scientific...

  • Page 217: Adjusting The Transfer Line Temperature

    Increase the source temperature as well to improve response for high-boiling compounds and prevent column bleed and matrix compounds from dirtying the ion source. Note The transfer line temperature and ion source temperature should be similar to avoid contaminating the ion source. Thermo Scientific ISQ 7610 User Guide...

  • Page 218: Modifying An Automatic Tune

    Optimizing Your Method Modifying an Automatic Tune Modifying an Automatic Tune ISQ 7610 AutoTune is a utility that uses certain parameters in the tune types to optimize system performance when generating a tune file.  To modify an automatic tune in Xcalibur software 1.
  • Page 219 Optimizing Your Method Modifying an Automatic Tune Figure 194. Accessing the Tune Type Editor from ISQ 7610 AutoTune 3. In the Tune Types dialog box, select a tune type to edit and click the Copy button. See Figure 195. Figure 195. Using the Tune Types Dialog Box 4.
  • Page 220 Select Last Saved to use a tune file saved on the instrument by the most recent successful automatic tune. A tune file may also be loaded and saved to the instrument for the Last Saved tune file using ISQ 7610 Manual Tune. –...
  • Page 221 During this time, the Busy light will be lit and you have to wait until the light goes off before using the ISQ 7610 system’s software. • CI Gas Flow (enabled when you select a CI Gas Type)—Use this field to enter the flow rate of your CI gas.
  • Page 222 • Default—Select this option to use the default electron lens positive voltage, which is 5 V. • Tune File—Select this option to use the value used in the tune file that you selected using the General tab. ISQ 7610 User Guide Thermo Scientific...
  • Page 223 (if anything) should be done about the resolution of the peak and any errors that occur. See Figure 198. Note Click the Advanced Settings check box to access the Lenses tab. Thermo Scientific ISQ 7610 User Guide...
  • Page 224 These three lenses act as a focusing element to maximize the ion beam intensity that is entering the ion guide. This field is typically set near the same voltage as lens 1. ISQ 7610 User Guide Thermo Scientific...
  • Page 225 – 10—Select this option to measure the width at 10% of the peak height. – 50—Select this option to measure the width at 50% of the peak height. Thermo Scientific ISQ 7610 User Guide...
  • Page 226 • Tune Resolution—Select this option to tune the resolution by itself. This resolution will tune the system at 100 and 1000 amu/s scan rates. You may also tune at a higher scan rate. See Figure 199. ISQ 7610 User Guide Thermo Scientific...
  • Page 227 100 and 1000 amu/s default scan rates. 8. Click the Targets tab to configure how you want to tune your target ion ratios. Target tuning is used to adjust the way the ISQ 7610 instrument tunes to meet regulatory requirements. See Figure 200.
  • Page 228 Figure 200. Configuring the Target Ion Ratios • Tune Target Ion Ratios—Select this checkbox to adjust the ratios based on the results from an injection of tuning compound. 9. Configure the options under the Detector tab. See Figure 201. ISQ 7610 User Guide Thermo Scientific...
  • Page 229 – Intensity—Use this field to enter the intensity you want to see on the tune report. 10. Click the Diagnostics tab and select a test to confirm the operational ability of the ISQ 7610 system. See Figure 202. Thermo Scientific...
  • Page 230 Optimizing Your Method Modifying an Automatic Tune Figure 202. Accessing the ISQ 7610 System Diagnostics 11. Click the Report tab configure how you want to view your data. See Figure 203. ISQ 7610 User Guide Thermo Scientific...
  • Page 231 Your new tune type is now in the Tune Types window. 13. Click Print to save your tune type information as a PDF or to print a hard copy of the information. See Figure 204. Thermo Scientific ISQ 7610 User Guide...
  • Page 232 14. Click Close to return to the Method Setup window. Now the tune type can be selected during an automatic tune.  To modify an automatic tune in Chromeleon software 1. From the ISQ epanel, select Tuning | Advanced AutoTune on the ISQ 7610 Dashboard. Figure 205. ISQ 7610 User Guide...
  • Page 233 2. The Tuning: Detector ISQ window opens. Click Advanced AutoTune. See Figure 206. Figure 206. Accessing the Tune Type Editor in Chromeleon Software 3. The AutoTune: Detector ISQ window opens. Click Tune Types. See Figure 207. Thermo Scientific ISQ 7610 User Guide...
  • Page 234 4. In the SelectTuneTypeForm dialog box, select a tune type to edit and click the Copy button. See Figure 208. Figure 208. Using the SelectTuneTypeForm Dialog Box 5. Configure the options under the General tab. See Figure 209. ISQ 7610 User Guide Thermo Scientific...
  • Page 235 Select Last Saved to use a tune file saved on the instrument by the most recent successful automatic tune. A tune file may also be loaded and saved to the instrument for the Last Saved tune file using ISQ 7610 Manual Tune. –...
  • Page 236 During this time, the Busy light will be lit and you have to wait until the light goes off before using the ISQ 7610 system’s software. • CI Gas Flow (enabled when you select a CI Gas Type)—Use this field to enter the flow rate of your CI gas.
  • Page 237 45 V above the voltage applied to the filament in order for the instrument software to allow the filament to be turned on. The voltage applied to the filament is the same number, but the opposite sign, of the electron energy. The settable range is 0–150 V. Thermo Scientific ISQ 7610 User Guide...
  • Page 238 (if anything) should be done about the resolution of the peak and any errors that occur. See Figure 211. Note Click the Advanced Settings check box to access the Lenses tab. ISQ 7610 User Guide Thermo Scientific...
  • Page 239 These three lenses act as a focusing element to maximize the ion beam intensity that is entering the ion guide. This field is typically set near the same voltage as lens 1. Thermo Scientific ISQ 7610 User Guide...
  • Page 240 – 10—Select this option to measure the width at 10% of the peak height. – 50—Select this option to measure the width at 50% of the peak height. ISQ 7610 User Guide Thermo Scientific...
  • Page 241 100 and 1000 amu/s scan rates. You may also tune at a higher scan rate. See Figure 212. Figure 212. Configuring the Tune Resolution • Mass—Use this pull-down menu to select the ion to be used for tuning. Thermo Scientific ISQ 7610 User Guide...
  • Page 242 100 and 1000 amu/s default scan rates. 9. Click the Targets tab to configure how you want to tune your target ion ratios. Target tuning is used to adjust the way the ISQ 7610 instrument tunes to meet regulatory requirements. See Figure 213.
  • Page 243 – Intensity—Use this field to enter the intensity you want to see on the tune report. 11. Click the Diagnostics tab and select a test to confirm the operational ability of the ISQ 7610 system. See Figure 215. Thermo Scientific...
  • Page 244 Optimizing Your Method Modifying an Automatic Tune Figure 215. Accessing the ISQ 7610 System Diagnostics 12. Click the Report tab configure how you want to view your data. See Figure 216. Figure 216. Configuring the Report Options • Acquire Full-Scan Spectrum—Select this checkbox to display the full spectrum on your tune report.
  • Page 245 14. Click Print to save your tune type information as a PDF or to print a hard copy of the information. See Figure 217. Figure 217. Printing the Tune Type Information 15. Click Close to return to the Method Setup window. Now the tune type can be selected during an automatic tune. Thermo Scientific ISQ 7610 User Guide...

  • Page 247: Chapter 9 Troubleshooting

    Troubleshooting section of the ISQ 7610 Hardware Manual. CAUTION ELECTRICAL SHOCK HAZARD: When troubleshooting any issue that requires removing a cover on the ISQ 7610 MS, you should power-off and vent the instrument to avoid any harm to yourself.

  • Page 248: Setting Instrument Conditions For Troubleshooting

    Setting Instrument Conditions for Troubleshooting A good first step for troubleshooting is to run a tune on the ISQ 7610 system. If you have good ion intensities, good peak shapes, and no air leak, you might want to look first at the GC, autosampler, or carrier gas.

  • Page 249: Checking Air/Water Spectra

    • Air/Water Check — Water (m/z 18/69) < 240% Once you have applied the settings in this section, and have allowed the ISQ 7610 system to equilibrate, run an EI diagnostic tune even if you cannot see any ion intensities.
  • Page 250 Troubleshooting Checking Air/Water Spectra Figure 218. Typical ISQ 7610 Air/Water Spectrum with Helium Carrier Gas at 1.2mL/min Note If using the AEI source, the air & water background ions may be too intense to observe without saturating the detector. Do not continue to scan when the detector is saturated or it will reduce detector lifetime.
  • Page 251 4. Using hydrogen as a carrier gas changes the air/water spectrum on the ISQ 7610 system. It general more background peaks due to the increased reactivity of the hydrogen gas with the components inside of the sample path.
  • Page 252 Checking Air/Water Spectra If any of the previous conditions are not met and a leak is suspected as the root cause, follow “An air leak has been detected” in the “Investigating Vacuum Issues” section of the ISQ 7610 Hardware Manual or “How to Know When Your System Needs Maintenance”...
  • Page 253 Troubleshooting Checking Air/Water Spectra Figure 221. Typical Air/Water Spectrum of an ISQ 7610 System with an Incorrectly Installed Ion Source Cartridge Figure 222 is an example of a system with no column flow. Thermo Scientific ISQ 7610 User Guide...
  • Page 254 Figure 223 is an example of a spectrum with a CI tune file run with an EI ion source. Figure 223. Typical Air/Water Spectrum of an ISQ 7610 System with an EI Ion Source Run with a CI Tune File...

  • Page 255: No Ions Present In Scans

    To find the version of firmware installed on your ISQ 7610 system 1. In Xcalibur software, on the ISQ 7610 Dashboard, click Air & Water/Tune. From the ISQ ePanel in Chromeleon software, click Tuning | Start Air & Water/Tune. This opens the Manual Tune application.
  • Page 256 Troubleshooting Checking the ISQ 7610 System Firmware Figure 224. The ISQ Manual Tune Application 2. Click Firmware in the upper right-hand corner of the home screen in ISQ 7610 Manual Tune. 3. A new menu opens. Click Version Info. 4. A dialog box containing opens that lists the firmware versions for the ISQ 7610 instrument control drivers, the lens driver board, and the controller interface board.
  • Page 257 Troubleshooting Checking the ISQ 7610 System Firmware Figure 225. Determining the Firmware Versions Note Depending on the version of ISQ Series instrument control software you are running, the controller interface board and lens driver board firmware may not be listed.

  • Page 258: How To Know When Your System Needs Maintenance

    Figure 226 shows a typical full scan background spectrum on an ISQ 7610 system. Figure 226. Typical Full Scan Background Spectrum of an ISQ 7610 System A typical clean system meets the following: • Detector gain — 3 × 10 •...
  • Page 259 • Mass assignments are correct. Review the tune report for true mass assignment values. • No extraneous peaks indicating contamination are present. Some of the most common reasons and indications your instrument needs maintenance are as follows: Thermo Scientific ISQ 7610 User Guide...
  • Page 260 151, 153 Trichloroethane Cleaning solvent Plasticizer (phthalates) Use of vinyl or plastic gloves Peaks spaced 14 amu apart Hydrocarbons Fingerprints, foreline pump oil, or other hydrocarbons Figure 228 shows an atypical PFTBA profile spectrum. ISQ 7610 User Guide Thermo Scientific...
  • Page 261 228, the atypical results are as follows: • Maximum intensity < 8 × 10 (8,000,0000) • m/z 207 from column bleed is prominent relative to PFTBA ions, indicating low calibration gas levels • Many small contamination peaks present Thermo Scientific ISQ 7610 User Guide...

  • Page 262: Investigating Baseline Issues

    Impurities have Check impurity levels in the gas source. accumulated in column Use correct gas purity. Abnormal rise in Impurities have Recondition or replace the column. baseline when oven accumulated in column temperature is high ISQ 7610 User Guide Thermo Scientific...
  • Page 263 Reduce oven temperature to the than column’s maximum maximum allowable temperature of the allowable temperature column. Leak at column fittings Find leak. Tighten fittings if loose. Replace ferrule if overtightened. Transfer line temperature is not set too low. Thermo Scientific ISQ 7610 User Guide...

  • Page 264: Investigating Peak Issues

    GC effluent will contact the inside wall. Fronting General Column is overloaded Decrease the injected amount and/or analyte concentrations. Increase the split ratio. Use a column with a thicker film. ISQ 7610 User Guide Thermo Scientific...
  • Page 265 Source temperature is too If tailing occurs on late eluting compounds, it is likely the source or transferline temperature is too low. Poor column Changing the Column characterization information about checking for leaks and column flow. Thermo Scientific ISQ 7610 User Guide...
  • Page 266 Make sure the tune file is correct. collecting data Verify that the Busy light is on during acquisition. Close Xcalibur, open Instrument Configuration, press the Reset button on the ISQ 7610 instrument, wait ten seconds, close Instrument Configuration. ISQ 7610 User Guide Thermo Scientific...

  • Page 267: Investigating Results Issues

    Carefully meter the injected amount. suitable Use a clean, good-quality syringe. Ion source is dirty Clean the ion source and retune the instrument. Split flow or ratio control is Monitor the flow. inadequate Replace the in-line filter. Thermo Scientific ISQ 7610 User Guide...
  • Page 268 Be sure the GC is inhibited by the MS configured correctly and waits for contact closure by the autosampler. Column temperature is Check the main oven door and cooling unstable flap. Monitor the column temperature. ISQ 7610 User Guide Thermo Scientific...

  • Page 269: Reconfiguring Your Instrument

    Thermo Foundation 3.1 | Instrument Configuration. When the Instrument Configuration utility opens, you can see an icon of the instruments you have connected. 2. Click the ISQ 7610 (and other instruments) icon in the Available Devices column and click Add to move it into the Configured Devices column.
  • Page 270 5. If you are running Chromeleon software on your system, click the Chromeleon Instrument Configuration Manager icon on your desktop to open the Chromeleon instrument configuration manager. See Figure 231. Figure 231. Opening the Chromeleon Instrument Configuration Manager ISQ 7610 User Guide Thermo Scientific...
  • Page 271 Troubleshooting Reconfiguring Your Instrument 6. Click the instrument name in left pane to open the Mass Spectrometer Configuration Editor. See Figure 232. Figure 232. Viewing the Mass Spectrometer Configuration Editor Thermo Scientific ISQ 7610 User Guide...
  • Page 272 8. Set the remote start. It is used to let the ISQ 7610 MS know when the GC has started a run. When you configure the GC, you can tell it what to send out to the ISQ 7610...
  • Page 273 Figure 234. Setting the Remote Start 9. Check the Hardware Inject Synchronization box to set the instrument to inject with the GC settings. This box is checked by default. See Figure 237. Figure 235. Configuring Hardware Inject Synchronization Thermo Scientific ISQ 7610 User Guide...
  • Page 274 11. If the mass spectrometer is equipped with a high dynamic range electron multiplier, check the High Dynamic Range Detection box. See Figure 237. Figure 237. Configuring the High Dynamic Range 12. Configure the Source Type. The choices are EI, CI or AEI. See Figure 238. ISQ 7610 User Guide Thermo Scientific...
  • Page 275 14. Click Communications to set the network IP address of the instrument’s PC communication board and assign a TCP port.The default settings are entered by the factory and should only be changed after consulting you local IT department and Thermo Scientific ISQ 7610 User Guide...
  • Page 276 Figure 240. Setting the Network IP Address and the TCP Port There is a reset button behind the front door of the ISQ 7610. Press and hold the reset button for more than 5 seconds to reset the instrument temporarily to the default IP address: 192.168.1.234.
  • Page 277 Troubleshooting Reconfiguring Your Instrument ammonia, you should change the oil every 30 days. Figure 241. Setting the Foreline Pump Oil Maintenance Interval Thermo Scientific ISQ 7610 User Guide...
  • Page 278 18. Check the Turbo Pump Oil/Bearing box to enable the maintenance reminder. Then set the number of days in which you want to be reminded to check the oil. The manufacturer recommends changing the oil cartridge every 730 days and the bearing every 1,460 days. Figure 243. ISQ 7610 User Guide Thermo Scientific...
  • Page 279 Figure 244. Setting the Filament 1 Maintenance Reminder 20. Check the Filament 2 box to enable the maintenance reminder. Then set the number of days in which you want to be reminded to check filament 2. In a leak-free system, the Thermo Scientific ISQ 7610 User Guide...
  • Page 280 filament should last between 30-360 days, depending on usage. See Figure 245 Figure 245. Setting the Filament 2 Maintenance Reminder 21. Check the Ion Source box to enable the maintenance reminder. Then set the number of days in which you want to be reminded to check the ion source. The time between...
  • Page 281 23. Click the Tuning tab and use the check boxes to enable Advanced AutoTune and the SmartTune application. You can also set the Intensity Check Pass Threshold and the Tune Report settings here. See Figure 248. Thermo Scientific ISQ 7610 User Guide...
  • Page 282 24. Click OK to return to the main Instrument Configuration window in both Xcalibur and Chromeleon software. 25. Click the Done button to close the Instrument Configuration utility in Xcalibur. 26. In Xcalibur software, you can check the status of your ISQ 7610 instrument in the Status tab of the ISQ Dashboard. See Figure...
  • Page 283 Troubleshooting Reconfiguring Your Instrument Figure 249. Checking the ISQ 7610 Instrument Status on the ISQ Dashboard 27. In the Status Pane of the Xcalibur Roadmap window, you can check the status of all your instruments. See Figure 251. Thermo Scientific...
  • Page 284 Figure 250. Checking All Instrument Statuses in Xcalibur Status Pane 28. You can view instrument status in Chromeleon software in the Messages Instrument Controller pane in the Chromeleon Instrument Configuration Manager. See Figure 251. ISQ 7610 User Guide Thermo Scientific...

  • Page 285: Upgrading The Software

    Note See the ISQ and TSQ GC-MS Spare Parts Guide for information about ordering software upgrades. 1. Ir you are running Xcalibur software, click Air & Water/Spectrum on the ISQ 7610 Dashboard to open the manual tune utility. See Figure 252.
  • Page 286 Troubleshooting Upgrading the Software Figure 252. ISQ 7610 Dashboard Manual Tune 1. If you are running Chromeleon software, Go to the ISQ tab on the Chromeleon Console and click Tuning to open the tuning window. See Figure 253. ISQ 7610 User Guide...
  • Page 287 Troubleshooting Upgrading the Software Figure 253. Accessing ISQ 7610 Tuning from the Chromeleon Console 2. The Tuning: Detector ISQ window opens. Click Start Air & Water/Tune. See Figure 254. Figure 254. Opening Manual Tune in Chromeleon Software 3. The ISQ Manual Tune application opens. Click Firmware on the upper right-hand side of the screen to open the firmware menu.
  • Page 288 256. Figure 256. Selecting the Upgrade Product Key Option Upgrade Product Key 5. The ISQ Product Key window opens. Enter your product key into the New Product Key box as shown in Figure 257. ISQ 7610 User Guide Thermo Scientific...
  • Page 289 Troubleshooting Upgrading the Software Figure 257. Entering the Product Key New Product Key Box Thermo Scientific ISQ 7610 User Guide...
  • Page 291 72, 106, 193, regulatory contaminants, common, confirming it is working, contamination, indications, exploring data, optimizing methods, creating a method powering on, reconfiguring, running a sample, data result issues, investigating, troubleshooting, Thermo Scientific ISQ 7610 User Guide...
  • Page 292 (carrier gas tank), checking, carrier gas tank pressure, profiled data, about, peak issues, results issues, transfer line temperature, tune report, raw file, selecting, ISQ 7610 User Guide Thermo Scientific...
  • Page 293 AEI Tunes CI- tune, CI+ tune, EI full tune, tuning using Autotune, 30–31, 53, with ISQ 7000 AutoTune, 30–31 user name, about, 168, using, vacuum, checking, V-lock source plug voltage of filament, setting, 192, Thermo Scientific ISQ 7610 User Guide...

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