Thermo Scientific GENESYS 30 User Manual page 11

Spectrophotometer
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Concentration Measurements
Set measurement
wavelength
Enter the conc. of the
standard
Select concentration units
for display
Select concentration mode
Thermo Scientific
Concentration measurements are based on measurement of a single standard solution of
known concentration. The instrument calculates a calibration based upon the absorbance of
the known standard and the assumption that there is zero absorbance at zero concentration. A
Factor is calculated from the data such that
Concentration = Factor x Absorbance
To perform a concentration measurement:
1. Select a measurement wavelength.
2. Set the Mode to C.
3. Select the units from the pop-up grid.
Follow the directions in the message bar.
4. Place a cuvette with a blank solution in the measurement position and press
5. Type the standard concentration in the Std. Conc. box and press
Absorbance of standard
solution
Calculated factor
.
to confirm.
GENESYS 30 User Guide
9

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