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How to perform a dilution
If the provided images do not deliver clinically actionable information, a 1:2 dilution should be
performed initially3. Increasing dilutions (1:4, 1:8) should be performed until images and/or
quantitative or semi-quantitative values provide clinical direction.
Dilution Ratio
Urine Volume
1:2
0.4 mL
1:4
0.2 mL
1:8
0.1 mL
When performing dilution:
• Use only sterile 0.9% NaCl to dilute urine samples
• Invert tube gently 10-15 times immediately prior to running the sample
NOTE: Diluting a urine sample may alter the sample pH and osmolality, which could lead to
changes in cellular appearance or the presence of non-pathogenic crystal formation. Analysis
should be performed immediately following dilution.
Sterile 0.9% NaCl (saline) Volume
0.4 mL
0.6 mL
0.7 mL
VE TS CA N SA US ER ' S M A N UA L
1 . 2 S OF T WA RE
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