VWR symphony 5.3 A Operating Instructions Manual
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VWR symphony 5.3 A Operating Instructions Manual

Co2 incubator
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Operating Instructions
CO
Incubator symphony 5.3 A
2
Valid: 06.2009 / 50111308 B

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Summary of Contents for VWR symphony 5.3 A

  • Page 1 Operating Instructions Incubator symphony 5.3 A Valid: 06.2009 / 50111308 B...
  • Page 2 This regulation does not apply to reproductions for in-plant use. Trademarks All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers. VWR International 1310 Goshen Parkway West Chester, PA 19380 Technical Support When contacting the Technical Support, please have the following information available: •...

  • Page 3: Table Of Contents

    Operating Instructions | symphony 5.3 A – 06/2009 Contents 1 Important safety informations 1.1 Instruction of the operating personnel..............7 1.2 Applicability of the instructions ................7 1.3 Warranty ......................... 7 1.4 Explanation of Signs ....................8 1.4.1 Symbols and signal words used in the operating instructions...... 8 1.4.2 Pictographs used in the operating instructions ..........
  • Page 4 Operating Instructions | symphony 5.3 A – 06/2009 Contents 6 Operation 6.1 Preparing the device .................... 34 6.2 Starting operation ....................34 7 Handling and control 7.1 Power switch ......................36 7.2 Operating panel....................36 7.3 Control self-test ....................38 7.4 Setting the nominal temperature value...............
  • Page 5 Operating Instructions | symphony 5.3 A – 06/2009 Contents 14 Device log 15 Certificate of decontamination...
  • Page 6 Operating Instructions | symphony 5.3 A – 06/2009 Figures Fig.1: Device dimensions ..................16 Fig.2: Lift point ....................... 16 Fig.3: Stacking......................17 Fig.4: General views of device ................19 Fig.5: Temperature sensor ..................21 Fig.6: Door switch....................21 Fig.7: Supply interfaces ..................22 Fig.8: Work space layout / Water reservoir ............

  • Page 7: Important Safety Informations

    VWR immediately for your own safety. 1.3 Warranty VWR warrants the operational safety and functions of the CO2 incubator only under the condition that: • the device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions, •...

  • Page 8: Explanation Of Signs

    Operating Instructions | symphony 5.3 A – 06/2009 Important safety informations Important safety informations 1.4 Explanation of Signs 1.4.1 Symbols and signal words used in the operating instructions WARNING Indicates a hazardous situation which, if not avoided, could result in...

  • Page 9: Signs On The Device

    Operating Instructions | symphony 5.3 A – 06/2009 Important safety informations Important safety informations 1.4.3 Signs on the device Saftey signs, test marks and marks of conformity placed on the device. Observe operating instructions! CSA test mark: Test certificate for USA/Canada, indicates that the product has met the requirements of nationally recognised product safety standards.
  • Page 10 Operating Instructions | symphony 5.3 A – 06/2009 Important safety informations Important safety informations NOTICE! Overpressure: The operating pressure of the gas applied to the device must not exceed 1 bar. If the gas is supplied at a higher pressure, the...
  • Page 11 Operating Instructions | symphony 5.3 A – 06/2009 Important safety informations Important safety informations • Observe the safety instructions of the manufacturer of the disinfectant and of the hygiene experts! Contamination hazard: The device can be used for preparing and processing infectious substances so that the device or device components may become contaminated.

  • Page 12: Intended Purpose Of The Device

    Operating Instructions | symphony 5.3 A – 06/2009 Important safety informations Important safety informations When using such disinfectants, avoid open flames or exposure to excessive heat during the entire disinfection process! • Use such disinfectants only in adequately ventilated rooms.

  • Page 13: Standards And Directives

    Operating Instructions | symphony 5.3 A – 06/2009 Important safety informations Important safety informations Do not use tissues, substances or liquids that: • are easily ignitible or explosive, • release vapors that form combustible or explosive mixtures when exposed to air, •...

  • Page 14: Delivery

    Operating Instructions | symphony 5.3 A – 06/2009 Delivery 2.1 Packaging The CO incubator symphony 5.3 A is delivered in a stable packaging box. Packaging materials All packaging materials can be separated and are reusable: • Packaging carton: Recycled paper •...

  • Page 15: Installation Of The Device

    In addition, heat dissipating from the device during permanent operation may cause a change in the room climate. • Therefore, the incubator symphony 5.3 A must only be installed in rooms with sufficient ventilation. • Do not install the device in room recesses without ventilation.

  • Page 16: Space Requirements

    Operating Instructions | symphony 5.3 A – 06/2009 Installation of the device Installation of the device Installation of the device 3.3 Space requirements Fig.1: When installing the device, make sure that the installation and supply connections are freely accessi- ble.

  • Page 17: Stacking

    Installation of the device Installation of the device 3.5 Stacking Fig.3: Two symphony 5.3 A devices can be stacked on top of each other. The stacking elements [1] and [2] secure the devices on top of each other. If the devices are placed onto mobile racks, ensure that the rollers [3] are secured by an arresting device during operation.

  • Page 18: Description Of The Device

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device 4.1 Views of device Front view: [1] Stacking elements Description front side [2] Plug caps [3] Glass door [4] Measuring cell with blower wheel and sensors [5] Door switch...

  • Page 19: Fig.4: General Views Of Device

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device Description of the device Fig.4: General views of device...

  • Page 20: Safety Devices

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device 4.2 Safety devices The device is equipped with the following safety devices: Safety features • A door switch interrupts the CO supply and the work space heating when the glass door is opened.

  • Page 21: Sensor System

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device Description of the device atmosphere. The CO content of the work space atmosphere can be controlled within a range of 0 - 20 %.

  • Page 22: Switchbox With Supply Interface

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device Description of the device The outer door can only be closed after the glass door has been latched properly. 4.6 Switchbox with supply interface All supply connections are installed in the switchbox at the rear of the device.

  • Page 23: Work Space Components

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device Description of the device is established via a cable with a connector for non- heating appliances. The holder for the two device fuses is integral to the power supply socket.

  • Page 24: Fig.9: Rear Panel Openings

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device Description of the device Rear panel openings: Fig.9: A sealable access port [1] allows cables, hoses or additional sensors to be routed into the work space of the device.

  • Page 25: C Decontamination Routine (Optional)

    Operating Instructions | symphony 5.3 A – 06/2009 Description of the device Description of the device 4.8 90 °C decontamination routine (optional) The optional 90 °C decontamination routine is used to decontaminate the Certified decontamination routine complete work space including all installed components and sensors.

  • Page 26: Start-Up

    Operating Instructions | symphony 5.3 A – 06/2009 Start-up 5.1 Preparing the work space The incubator is not delivered in a sterile state. Before the initial start-up, the device must be decontamina- ted. Before the decontamination is performed, the follo- wing work space components must be cleaned: •...

  • Page 27: Fig.11: Shelf Support/Sheet Insert Installation

    Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up Start-up Installing the shelf supports: 1. Fig.11: Insert the shelf supports [3] into the perfo- AInstalling the shelf supports: rations [1] of the support rail with the bar facing down.

  • Page 28: Gas Connection

    Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up Start-up 5.3 Gas connection NOTICE! Gas quality: must be of the following qualities: • Purity 99.5 % min, • medical grade. CAUTION Overpressure: The operating pressure of the gas applied to the device must not exceed 1 bar.

  • Page 29: Power Supply Connection

    Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up Start-up 5.4 Power supply connection WARNING Electric shock: Contact with current-carrying components may cause a lethal electric shock. Before connecting the device to the power supply, check plug and connection line for damage.
  • Page 30 Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up unassigned slot COM 1/COM 2 etc. at the PC. 4. Turn PC on. Transfer protocol: The interface must be configured as follows: 9600 baud, 8 data bits, 1 stop bit, no parity.
  • Page 31 Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up Faulty response from control unit: If a returned response is incomplete or faulty, the CPU responds with an NAK (15 , only 1 byte, without frame). Otherwise, the command code (with pertaining length information) is regarded as a response and the data that may be required is transmitted.

  • Page 32: Connecting The Alarm Contact

    Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up 5.6 Connecting the alarm contact NOTICE! Expert work: VWR warrants the operational safety and the operativeness of the device only if installation and repairs are performed pro- perly. The connection of the device to an external alarm system must...

  • Page 33: Fig.15: Example Of A Connection Scheme

    Operating Instructions | symphony 5.3 A – 06/2009 Start-up Start-up Start-up Connection example: Fig.15: The connector [5] for the connecting cable is comprised in the scope of delivery. The values for the operating voltage of the external circuits and of the fusing of the alarm system are given in the table above.

  • Page 34: Operation

    Operating Instructions | symphony 5.3 A – 06/2009 Operation 6.1 Preparing the device The device must only be released for operation after all major measures for the start-up have been taken (Section 5.1–5.7). Prior to starting operation, the following device components must be chek-...
  • Page 35 Operating Instructions | symphony 5.3 A – 06/2009 Operation Operation value, the humidity rises. 9. When the temperature and relative humidity are constant, automatic adjustment of the CO measuring system is performed. 10. The ”auto-start” indicator goes off. 11. The CO control supplies the set amount of CO 12.

  • Page 36: Handling And Control

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control 7.1 Power switch Fig.16: Depending on which side the door hinges are installed, the power Power switch intergrated in front switch is integral to the front cover of one of the front device stands.

  • Page 37: Fig.16: Power Switch / Operating Panel

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control Handling and control Fig.16: Power switch / Operating panel...

  • Page 38: Control Self-Test

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control 7.3 Control self-test After the device has been turned on, the control goes through a test rou- Test routine tine. 1. Turn the device on  Press power switch.

  • Page 39: Setting The Nominal Temperature Value

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control 7.4 Setting the nominal temperature value 1. Indicate the nominal value:  Press the  key.  The temperature display shows the current nominal value. 2. Enter the nominal value: Temperature nominal value The nominal value can be increased or reduced in increments;...

  • Page 40: Activating The Auto-Start Routine

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control 7.6 Activating the auto-start routine The auto-start function is an automated routine for the start and the subse- Auto-start function quent adjustment of the CO measuring system. After the start, the device control adjusts the temperature to the set nominal value while humidity is generated.

  • Page 41: Reading Failure Codes

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control 4. Close all device doors:  The temperature display shows the actual value, the CO display shows ”run”, the ”door” LED goes off. 5. Cancel the auto-start routine: ...
  • Page 42 Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control Delay time reset: If the set nominal value is reached during the specified period, the delay time is reset to 1 min! Failure cause: Failure messages...
  • Page 43 Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control 4. Scroll through the failure code table: To read stored failure codes from the failure code table.  Keep the  key depressed.  To scroll, keep the  or  key depressed.
  • Page 44 Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control Handling and control Failure code list Code Description Cause Repair Communication between No values displayed display and CPU- main Contact Service ΞΞΞ board faulty NV RAM faulty,...

  • Page 45: Resetting The Thermal Protection

    Operating Instructions | symphony 5.3 A – 06/2009 Handling and control Handling and control 7.8 Resetting the thermal protection 1. The Thermal Protection Active LED illuminates: 2. Turn the device off:  Press the power switch.  All indicators come off.

  • Page 46: Shut-Down

    Operating Instructions | symphony 5.3 A – 06/2009 Shut-down 8.1 Shutting the device down WARNING Contamination hazard: If the work space surfaces are contaminated, germs my be transferred to the environment of the device. In case of a shut-down, the device must be decontaminated! 1.

  • Page 47: Cleaning And Disinfection

    Operating Instructions | symphony 5.3 A – 06/2009 Cleaning and disinfection 9.1 Decontamination procedures The operator must prepare hygiene regulations for the decontamination of the device in accordance with the application of the device. Wipe/spray disinfection: is used as the standardized manual disinfection procedure for the device and for all accessories.
  • Page 48 Operating Instructions | symphony 5.3 A – 06/2009 Cleaning and disinfection Cleaning and disinfection Recommended disinfectant: A surface disinfectant recommended by VWR can be ordered under follo- Types of disinfectant wing part numbers: • Spray bottle, 250 ml: Part No.: 50052425 •...

  • Page 49: Fig.17: Removing Blower Wheel And Cover

    Operating Instructions | symphony 5.3 A – 06/2009 Cleaning and disinfection Cleaning and disinfection Cleaning and disinfection fused socket. 4. Pump water out of water tray. 5. Disconnect connector from socket and remove pump from rear panel. 6. Wipe remaining water off using a cloth.

  • Page 50: C Decontamination Routine (Optional)

    Operating Instructions | symphony 5.3 A – 06/2009 Cleaning and disinfection Cleaning and disinfection Cleaning the work space and accessories: Finishing disinfection 1. Thoroughly remove dirt residues and deposits using a solution of tepid water and dishwashing agent. 2. Wipe surfaces clean using a clean cloth and plenty of clean water.

  • Page 51: C Decontamination Routine Procedure

    Operating Instructions | symphony 5.3 A – 06/2009 Cleaning and disinfection Cleaning and disinfection Cleaning and disinfection 9.3.1 90 °C decontamination routine procedure Fig.18: The routine is divided into four phases. Each individual phase or several phases can be cancelled (i.e.

  • Page 52: Activating The 90 °C Decontamination Routine

    Operating Instructions | symphony 5.3 A – 06/2009 Cleaning and disinfection Cleaning and disinfection 9.3.2 Activating the 90 °C decontamination routine Before running the decontamination routine, fill the water tray with 300 ml Preparation of decontamination of water. 1. Turn the device on: ...

  • Page 53: Maintenance

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance 10.1 Inspections and checks To ensure the operativeness and the operational safety of the device, the functions and device components listed below must be checked at diffe- rent intervals. Daily check: Check intervals •...

  • Page 54: Fig.19: Temperature Calibration

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance Maintenance Maintenance If a major temperature deviation is found during this check, a temperature calibration is required. During this process, the temperature control of the device is set to the value measured during the tempe- rature comparison measurement.

  • Page 55: Temperature Calibration Procedure

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance Maintenance 10.4 Temperature calibration procedure 1. Activate calibration: Temperature calibration steps  Keep the  key depressed for 5 seconds.  All operating panel indicators flash. 2. Indicate the nominal value: ...

  • Page 56: Preparing The Co Calibration

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance Maintenance Maintenance 10.5 Preparing the CO calibration To determine the exact measured value of the device- integral CO sensor, a CO comparison measurement may be performed every three months. If a major deviation is found during this check, a CO calibration is required.

  • Page 57: Co Calibration Procedure

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance Maintenance 10.6 CO calibration procedure 1. Activate the calibration: calibration steps  Keep the  key depressed for 5 seconds.  All operating panel indicators flash. 2. Indicate the nominal value: ...

  • Page 58: Replacing The Sterile Filters

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance Maintenance Maintenance 10.7 Replacing the sterile filters The sterile filters have plastic threads and are screwed by hand into the threaded hole on the control box. Procedure for gas supply sterile filter: 1.

  • Page 59: Replacing The Door Seal

    Operating Instructions | symphony 5.3 A – 06/2009 Maintenance Maintenance Maintenance 10.9 Replacing the door seal The door seal (magnetic seal) of the outer door is loca- ted in the retaining slot. No tools are required to replace the seal.

  • Page 60: Parts And Accessories

    • Date Purchased. NOTE! Repairs: Use only original parts that have been tested and approved by VWR. The use of other parts presents potential hazards and will make the warranty void! Additional parts: For additional parts please contact VWR Service Organisation.

  • Page 61: Technical Data

    Operating Instructions | symphony 5.3 A – 06/2009 Technical data Mechanical / thermal data: Description Unit Value Mechanical External dimensions 637 x 867 x 766 Width x Depth x Height 25.1 x 34.1 x 30.2 Interior dimensions 470 x 607 x 530 Width x Depth x Height 18.5 x 23.9 x 20.9...
  • Page 62 Operating Instructions | symphony 5.3 A – 06/2009 Technical data Technical data Technical data Gas supply/ electrical system / others: Description Unit Value gas supply system Gas purity min. 99.5 or medical quality Prepressure min. 0.8 - max. 1 Measuring and control range % vol.

  • Page 63: Fig.24: Co 2 Consumption

    Operating Instructions | symphony 5.3 A – 06/2009 Technical data Technical data Technical data Average gas consumption: Fig.24: CO 2 consumption...
  • Page 64 Operating Instructions | symphony 5.3 A – 06/2009 Disposal CAUTION Contamination hazard: The device can be used for preparing and processing infectious Preparation for disposal substances so that the device or device components may become contaminated. Before device components are discarded, they must be decon- taminated! •...
  • Page 65 Operating Instructions | symphony 5.3 A – 06/2009 Disposal Disposal Disposal List of used materials: Component Material Thermal insulation components Polystyrene foam EPS/PPS-Compound Enclosed electrical components coated with different Printed circuit boards plastics, equipped on epoxy resin-bound boards. Plastic components, general...
  • Page 66 Operating Instructions | symphony 5.3 A – 06/2009 Device log Record following informations here: • nameplate information, • work carried out, • maintenance work, • and repairs Device type: Part number: Serial number: Service number: Location: Operator's note: Work carried out...
  • Page 67 Operating Instructions | symphony 5.3 A – 06/2009 Certificate of decontamination...
  • Page 68 www.vwrsp.com...

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